Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,249,224 C→T 7.8% D17N (GAT→AAT)  ruvB ← ATP‑dependent DNA helicase, component of RuvABC resolvasome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,249,2240CT 7.8% 204.7 / 5.7 64D17N (GAT→AAT) ruvBATP‑dependent DNA helicase, component of RuvABC resolvasome
Reads supporting (aligned to +/- strand):  ref base C (33/26);  new base T (2/3);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCAT  >  minE/1249154‑1249284
                                                                      |                                                            
tgtgAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATc                                                              >  1:366561/1‑71 (MQ=255)
  tgAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCtt                                                            >  1:1036812/1‑71 (MQ=255)
             gCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCaaa                                                    >  1:1092126/1‑68 (MQ=255)
             gCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAg                                                   >  1:2963657/1‑69 (MQ=255)
                 cTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTAc                                             >  1:979854/1‑71 (MQ=255)
                 cTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTAc                                             >  1:2906836/1‑71 (MQ=255)
                 cTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTAc                                             >  1:2696639/1‑71 (MQ=255)
                    aCCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCgg                                          <  1:3205446/71‑1 (MQ=255)
                    aCCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCgg                                          <  1:2694610/71‑1 (MQ=255)
                      cAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCgg                                          >  1:2285127/1‑69 (MQ=255)
                        aCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCa                                        >  1:893589/1‑69 (MQ=255)
                        aCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAg                                       >  1:1021977/1‑70 (MQ=255)
                        aCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAg                                       >  1:956436/1‑70 (MQ=255)
                        aCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAg                                       >  1:1448931/1‑70 (MQ=255)
                        aCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAg                                       >  1:858487/1‑70 (MQ=255)
                        aCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGa                                      <  1:1205684/71‑1 (MQ=255)
                        aCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGa                                      <  1:3784065/71‑1 (MQ=255)
                        aCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGa                                      <  1:3242277/71‑1 (MQ=255)
                             ctctTCCAGTAATTTGGGGCGAATTGCGCGATCTGCTACATCTTCCGGCaa                                                     <  1:2044244/51‑1 (MQ=255)
                             ctctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCaa                                                     <  1:3870452/51‑1 (MQ=255)
                             ctctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCaa                                                     <  1:421315/51‑1 (MQ=255)
                             ctctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCaa                                                     <  1:588770/51‑1 (MQ=255)
                             ctctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCaa                                                     <  1:2701752/51‑1 (MQ=255)
                               catCCAGTAATTTTGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGa                                      <  1:444245/62‑1 (MQ=255)
                                  ccAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACgg                            >  1:417071/1‑71 (MQ=255)
                                  ccAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACgg                            >  1:78105/1‑71 (MQ=255)
                                  ccAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACg                             <  1:3396885/70‑1 (MQ=255)
                                  ccAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACg                             <  1:3266189/70‑1 (MQ=255)
                                  ccAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACg                             <  1:977671/70‑1 (MQ=255)
                                     gTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCaa                                                     <  1:1197626/43‑1 (MQ=255)
                                     gTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCt                         >  1:2211555/1‑71 (MQ=255)
                                     gTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCt                         >  1:3836454/1‑71 (MQ=255)
                                        aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCt                      >  1:593851/1‑71 (MQ=255)
                                        aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCt                      >  1:211115/1‑71 (MQ=255)
                                        aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCt                      >  1:2997514/1‑71 (MQ=255)
                                          ttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCaaa                                                    >  1:208940/1‑39 (MQ=255)
                                          ttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCaaa                                                    >  1:4039378/1‑39 (MQ=255)
                                          ttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCaaa                                                    >  1:1156280/1‑39 (MQ=255)
                                          ttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCaaa                                                    >  1:1697962/1‑39 (MQ=255)
                                          ttGGGGCGAATGGCCCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGt                           >  1:837909/1‑64 (MQ=255)
                                           tGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTc                    <  1:2881625/70‑1 (MQ=255)
                                           tGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTc                    <  1:1725700/70‑1 (MQ=255)
                                           tGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTc                    <  1:2176564/70‑1 (MQ=255)
                                           tGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTc                    <  1:3535511/70‑1 (MQ=255)
                                           tGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTc                    <  1:2341814/70‑1 (MQ=255)
                                                  aaTGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGa                               >  1:1205482/1‑52 (MQ=255)
                                                  aaTGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGa                               >  1:17477/1‑52 (MQ=255)
                                                  aaTGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGa                               >  1:3998171/1‑52 (MQ=255)
                                                  aaTGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGa                               >  1:2919856/1‑52 (MQ=255)
                                                  aaTGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGa                               >  1:2853843/1‑52 (MQ=255)
                                                   aTGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTAc                                             >  1:1274793/1‑37 (MQ=255)
                                                   aTGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTAc                                             >  1:131482/1‑37 (MQ=255)
                                                   aTGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTAc                                             >  1:2356805/1‑37 (MQ=255)
                                                   aTGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTAc                                             >  1:479982/1‑37 (MQ=255)
                                                      gcgcGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTa        >  1:3427844/1‑71 (MQ=255)
                                                      gcgcGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTa        <  1:414418/71‑1 (MQ=255)
                                                           aTCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGa                                      <  1:2866464/36‑1 (MQ=255)
                                                               gCTACATTTTCCGGCAAAGTGGTACCGGCAGAAAt                                   >  1:612542/1‑35 (MQ=255)
                                                                cTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCAt  <  1:1610586/67‑1 (MQ=255)
                                                                     tCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACg                             <  1:573404/35‑1 (MQ=255)
                                                                     tCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACg                             <  1:556975/35‑1 (MQ=255)
                                                                     tCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACg                             <  1:2793949/35‑1 (MQ=255)
                                                                     tCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACg                             <  1:1188079/35‑1 (MQ=255)
                                                                     tCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACg                             <  1:2917902/35‑1 (MQ=255)
                                                                     tCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACg                             <  1:1052311/35‑1 (MQ=255)
                                                                      cTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAatca               >  1:2474660/1‑48 (MQ=255)
                                                                      |                                                            
TGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCAT  >  minE/1249154‑1249284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑