| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,257,376 | T→C | 13.0% | F7L (TTT→CTT) | yecP → | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,257,376 | 0 | T | C | 13.0% | 200.8 / 19.8 | 77 | F7L (TTT→CTT) | yecP | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (31/36); new base C (2/8); total (33/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTG > minE/1257307‑1257446 | ccAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACtt > 1:925010/1‑71 (MQ=255)ccAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACtt > 1:816527/1‑71 (MQ=255) cAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACttt < 1:2735571/71‑1 (MQ=255) cAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACttt < 1:3580764/71‑1 (MQ=255) cAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACttt < 1:1462675/71‑1 (MQ=255) aGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACttt > 1:3723678/1‑70 (MQ=255) gTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTa < 1:4110750/71‑1 (MQ=255) tGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACtt > 1:1754632/1‑67 (MQ=255) tGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACtt > 1:1048857/1‑67 (MQ=255) tGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTAt < 1:2819500/71‑1 (MQ=255) tGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTAt < 1:260184/71‑1 (MQ=255) tGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTAt < 1:1780242/71‑1 (MQ=255) gCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACttt < 1:2348311/67‑1 (MQ=255) cTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATtc > 1:4106718/1‑71 (MQ=255) cTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATtc > 1:2257996/1‑71 (MQ=255) aaCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTctct < 1:288201/70‑1 (MQ=255) aCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTctct < 1:3549258/69‑1 (MQ=255) ttGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTctct < 1:120175/66‑1 (MQ=255) ggTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGc > 1:2470649/1‑70 (MQ=255) cACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCaaa > 1:838876/1‑70 (MQ=255) aCTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGCTAACTTTTa < 1:3044617/53‑1 (MQ=255) ggtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATtc < 1:1236698/53‑1 (MQ=255) ggtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTctct < 1:1750163/56‑1 (MQ=255) gtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATtct < 1:3112947/53‑1 (MQ=255) gtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATtct < 1:1536368/53‑1 (MQ=255) gtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATtct < 1:2228837/53‑1 (MQ=255) gtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAatc < 1:3626723/69‑1 (MQ=255) ggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTctct > 1:1145980/1‑53 (MQ=255) ggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCt > 1:361394/1‑71 (MQ=255) gCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACctt < 1:3293534/44‑1 (MQ=255) gCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACctt < 1:383499/44‑1 (MQ=255) gCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACctt < 1:1756648/44‑1 (MQ=255) gCATTAAAAGCAGAGGACGCTCCATGATCGACTTTTGTAACctt < 1:214632/44‑1 (MQ=38) cATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:1726009/71‑1 (MQ=255) cATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:3945364/71‑1 (MQ=255) ttAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:1863548/69‑1 (MQ=255) ttAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:2485697/69‑1 (MQ=255) tAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTca > 1:3953559/1‑70 (MQ=255) aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACtt > 1:319264/1‑38 (MQ=255) aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:3527549/1‑71 (MQ=255) aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:1414576/1‑71 (MQ=255) aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:1461827/1‑71 (MQ=255) aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:2407644/1‑71 (MQ=255) aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:313938/1‑71 (MQ=255) gagGACGCTGCTTGATCGACTTTGGTATGTTTTAt < 1:906330/35‑1 (MQ=25) ggACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGa < 1:1585102/40‑1 (MQ=255) ggACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGa < 1:3643891/71‑1 (MQ=255) gACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGa < 1:937862/70‑1 (MQ=255) tGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt > 1:2219788/1‑52 (MQ=255) tGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt > 1:862631/1‑52 (MQ=255) tGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt > 1:1723460/1‑52 (MQ=255) gCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTca > 1:2503168/1‑53 (MQ=255) gCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTca > 1:3557578/1‑53 (MQ=255) tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaaa < 1:1798883/38‑1 (MQ=255) tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaaa < 1:1226324/38‑1 (MQ=255) tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaaa < 1:1305596/38‑1 (MQ=255) tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaaa < 1:3652085/38‑1 (MQ=255) tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaaa < 1:1792782/38‑1 (MQ=255) tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaaa < 1:3873886/38‑1 (MQ=255) tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaaa < 1:2278843/38‑1 (MQ=255) tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAatcat < 1:410757/44‑1 (MQ=255) aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCt > 1:3128820/1‑44 (MQ=255) aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCt > 1:2354633/1‑44 (MQ=255) aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcgc > 1:738914/1‑71 (MQ=255) aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcgc > 1:423041/1‑71 (MQ=255) aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcgc > 1:2970144/1‑71 (MQ=255) aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcgc > 1:1212705/1‑71 (MQ=255) aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcgc > 1:1907653/1‑71 (MQ=255) aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcgc > 1:2702683/1‑71 (MQ=255) aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcg > 1:874116/1‑70 (MQ=255) aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcg > 1:838539/1‑70 (MQ=255) aTCGACTTTGGTAACTTTTATTCTATGATTGCCAAAAatcat > 1:51322/1‑42 (MQ=255) tCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAatcat < 1:447498/41‑1 (MQ=255) tCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:870736/45‑1 (MQ=255) tCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:1772089/45‑1 (MQ=255) gACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:342719/71‑1 (MQ=255) gACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:2040894/71‑1 (MQ=255) tttGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:1720377/40‑1 (MQ=255) tGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:3060878/1‑42 (MQ=255) tGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:4092567/1‑42 (MQ=255) tGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:868536/1‑42 (MQ=255) tGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcac > 1:3170983/1‑41 (MQ=255) tGGTAACTTTTATTCTCTGATTGACAAAAATCATCTTTcaca > 1:875191/1‑42 (MQ=255) gTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCt < 1:1462119/71‑1 (MQ=255) gTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCt < 1:1113790/71‑1 (MQ=255) tAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTa < 1:3857218/71‑1 (MQ=255) gaaCTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:2682795/34‑1 (MQ=255) aCTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAAc < 1:2773737/71‑1 (MQ=255) aCTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAAc < 1:3464387/71‑1 (MQ=255) aCTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAAc < 1:3661786/71‑1 (MQ=255) cTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACt < 1:3558670/71‑1 (MQ=255) ttttATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:683280/59‑1 (MQ=255) ttttATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:2138883/59‑1 (MQ=255) ttttATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTg > 1:2336597/1‑71 (MQ=255) | CCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTG > minE/1257307‑1257446 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |