| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,291,126 | G→A | 30.1% | intergenic (+241/+212) | yeeN → / ← asnW | conserved hypothetical protein/tRNA‑Asn |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,291,126 | 0 | G | A | 30.1% | 122.9 / 67.5 | 83 | intergenic (+241/+212) | yeeN/asnW | conserved hypothetical protein/tRNA‑Asn |
| Reads supporting (aligned to +/- strand): ref base G (26/32); new base A (14/11); total (40/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
AATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGG > minE/1291056‑1291181 | aaTTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTg < 1:1578804/71‑1 (MQ=255) aCGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACg > 1:1839434/1‑71 (MQ=255) aCGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACg > 1:1351895/1‑71 (MQ=255) tAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACt > 1:3950507/1‑71 (MQ=255) tCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCAt < 1:2168246/70‑1 (MQ=255) aaaGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACa > 1:2174315/1‑69 (MQ=255) aaGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTa > 1:65463/1‑51 (MQ=255) aaGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTa > 1:2038542/1‑51 (MQ=255) aaGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTa > 1:1944756/1‑51 (MQ=255) aaGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACagag > 1:2290692/1‑71 (MQ=255) aaGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACagag > 1:3525265/1‑71 (MQ=255) aaGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACagag > 1:1813838/1‑71 (MQ=255) cgcgATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGtttt < 1:1813433/71‑1 (MQ=255) aTAGCGACATCCCGCATAAGTCATTTTTCTCTTTATGTTTGTACgg < 1:2095329/46‑1 (MQ=38) aTAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACgg < 1:3382369/46‑1 (MQ=255) aGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCt > 1:2561528/1‑71 (MQ=255) aGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCt > 1:2537364/1‑71 (MQ=255) aGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCt > 1:2153876/1‑71 (MQ=255) aCATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCg < 1:2978425/71‑1 (MQ=255) tCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTc < 1:3131207/43‑1 (MQ=255) tCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTc < 1:3050810/43‑1 (MQ=255) tCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTc < 1:373787/43‑1 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACaga > 1:334757/1‑51 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACaga > 1:3958086/1‑51 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACaga > 1:2388721/1‑51 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATc < 1:1032458/70‑1 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCg > 1:2919797/1‑71 (MQ=255) ccGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCg < 1:2130092/71‑1 (MQ=255) cGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:844462/71‑1 (MQ=255) tAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTc < 1:526011/36‑1 (MQ=255) tAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATg < 1:1537061/71‑1 (MQ=255) tAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATg < 1:1139450/71‑1 (MQ=255) tAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACa < 1:3969702/44‑1 (MQ=255) tAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACa < 1:743652/44‑1 (MQ=255) aaGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACa > 1:2583324/1‑43 (MQ=255) aaGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGa > 1:1781609/1‑68 (MQ=255) aaGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGa > 1:594269/1‑68 (MQ=255) gCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTg > 1:795521/1‑71 (MQ=255) gCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTg > 1:781663/1‑71 (MQ=255) tttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCt < 1:1463682/50‑1 (MQ=255) tttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCt < 1:1257617/50‑1 (MQ=255) tttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCt < 1:816074/50‑1 (MQ=255) tttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCTAATCGTGATGTCTg < 1:1009241/68‑1 (MQ=255) tttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTg < 1:3648756/68‑1 (MQ=255) tttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTg < 1:1948443/68‑1 (MQ=255) tttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTCCGAATCGTGATGTCTg < 1:4129088/68‑1 (MQ=255) tttttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGttttt < 1:2616622/45‑1 (MQ=255) tttttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGttttt < 1:1231775/45‑1 (MQ=255) tttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGttttt > 1:295317/1‑43 (MQ=255) tttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGaa > 1:2039172/1‑54 (MQ=255) tttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGaa > 1:1294825/1‑54 (MQ=255) tttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGaa > 1:2144294/1‑54 (MQ=255) tttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGaa > 1:148240/1‑54 (MQ=255) tttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTg > 1:3320159/1‑66 (MQ=255) tttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg > 1:1919531/1‑71 (MQ=255) tttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg > 1:542329/1‑71 (MQ=255) tttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg > 1:1814360/1‑71 (MQ=255) tttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTgg > 1:923750/1‑70 (MQ=255) tttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTgg > 1:9553/1‑70 (MQ=255) tttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTgg > 1:2312524/1‑70 (MQ=255) tttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTg > 1:3984047/1‑69 (MQ=255) tttCTCGTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTgg > 1:530072/1‑70 (MQ=255) ttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGa > 1:877855/1‑59 (MQ=255) ttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGa > 1:1594480/1‑59 (MQ=255) ctctTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGaa > 1:2517301/1‑51 (MQ=255) tttATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGa < 1:3881688/39‑1 (MQ=255) tttATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGa < 1:1489079/39‑1 (MQ=255) tttATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTTCGAATCGTGATGTCTGTTggg < 1:2644757/65‑1 (MQ=255) tttATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg < 1:3885850/65‑1 (MQ=255) tttATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg < 1:1719792/65‑1 (MQ=255) tttATCTTTATACGGTACTTCATGGAACAGAGttttt < 1:1253489/37‑1 (MQ=255) ttATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTgg < 1:3755763/63‑1 (MQ=255) ttATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTgg < 1:2840139/63‑1 (MQ=255) ttATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTgg < 1:1625122/63‑1 (MQ=255) ttATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTgg < 1:3573052/63‑1 (MQ=255) tttGTACGGTACTTCATGTAACAGAGTTTTTGACCTTGCGAATCGt < 1:2111514/46‑1 (MQ=255) tttGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:2927902/46‑1 (MQ=255) tttGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:580745/46‑1 (MQ=255) tttGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:725430/46‑1 (MQ=255) tttGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:2941569/46‑1 (MQ=255) tttGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:493553/46‑1 (MQ=255) tttGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:1048301/46‑1 (MQ=255) ttGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:1446008/45‑1 (MQ=255) tGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGa > 1:2384039/1‑46 (MQ=255) | AATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGG > minE/1291056‑1291181 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |