| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,408,722 | G→A | 37.1% | A331T (GCG→ACG) | yejF → | fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,408,722 | 0 | G | A | 37.1% | 30.3 / 33.9 | 35 | A331T (GCG→ACG) | yejF | fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (6/16); new base A (3/10); total (9/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
CTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAA > minE/1408656‑1408783 | cTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCgct < 1:3661995/71‑1 (MQ=255)cTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGACgct < 1:2217939/71‑1 (MQ=255) aGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCtgctgc < 1:2820745/71‑1 (MQ=255) aGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCtgctgc < 1:2534652/71‑1 (MQ=255) aGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCtgctgc < 1:1892244/71‑1 (MQ=255) tGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCtgctgc < 1:2550218/48‑1 (MQ=255) tGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCtgctgc < 1:94885/48‑1 (MQ=255) ggCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGcat > 1:3772550/1‑71 (MQ=255) gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCgct < 1:2086039/40‑1 (MQ=255) gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCgct < 1:618386/40‑1 (MQ=255) gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCgct < 1:3349550/40‑1 (MQ=255) gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCgct < 1:3084938/40‑1 (MQ=255) gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCtgctgc < 1:1620873/45‑1 (MQ=255) gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGcatc < 1:1578167/71‑1 (MQ=255) gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGAGCTGCTGCGACTGATTAATTCTCAGGGCAGcatc < 1:2506846/71‑1 (MQ=255) gTCCGGTTCCGGGAAAAGTACGACGGGACTGACGCTGCTGCGACTGATTAATtc < 1:2506459/54‑1 (MQ=255) gTCCGGTTCCGGGAAAAGTACGACGGGACTGACGCTGCTGCGACTGATTAATtc < 1:3841280/54‑1 (MQ=255) gTCCGGTTCCGGGAAAAGTACGACGGGACTGACGCTGCTGCGACTGATTAATtc < 1:2973838/54‑1 (MQ=255) gTCCGGTTCCGGGAAAAGTACGACGGGACTGACGCTGCTGCGACTGATTAATtc < 1:3546493/54‑1 (MQ=255) tCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCtt > 1:1212143/1‑71 (MQ=255) tCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCtt > 1:3445884/1‑71 (MQ=255) cGGTTCCGGGAAAAGTACGACGGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCttt < 1:139627/70‑1 (MQ=255) tCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACgg > 1:2230134/1‑71 (MQ=255) tCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACg > 1:4030512/1‑70 (MQ=255) ggAAAAGTACGACGGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCa > 1:2086416/1‑70 (MQ=255) ggAAAAGTACGACGGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAg > 1:107640/1‑71 (MQ=255) gAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGc < 1:238480/71‑1 (MQ=255) cgacgGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCa > 1:3158738/1‑70 (MQ=255) cgGGACTGGCGCTGCTGCGACTGATTAATTGTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCaaaa < 1:2278862/70‑1 (MQ=255) cgGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCaaaa < 1:2640787/70‑1 (MQ=255) aCTGGCGCTGCTGCGACTGATTAATTCTCAGGgcag > 1:1448079/1‑36 (MQ=255) cTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa < 1:333818/47‑1 (MQ=255) cTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa < 1:4087993/47‑1 (MQ=255) cTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa < 1:1835315/47‑1 (MQ=255) cTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa < 1:63777/47‑1 (MQ=255) | CTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAA > minE/1408656‑1408783 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |