| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,418,334 | G→A | 41.8% | V95I (GTC→ATC) | narP → | DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,418,334 | 0 | G | A | 41.8% | 48.3 / 97.3 | 79 | V95I (GTC→ATC) | narP | DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX |
| Reads supporting (aligned to +/- strand): ref base G (16/30); new base A (20/13); total (36/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.86e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
TTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAGTATTGCTG > minE/1418271‑1418402 | ttGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTc < 1:2851162/66‑1 (MQ=255)ttGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTc < 1:4069200/66‑1 (MQ=255)ttGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTc < 1:1464453/66‑1 (MQ=255) tGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTc < 1:532225/65‑1 (MQ=255) gcgcAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCTATGTCTTTgc < 1:2251669/69‑1 (MQ=255) gcgcAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTgcgc > 1:3780646/1‑71 (MQ=255) gcgcAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTgc < 1:2952815/69‑1 (MQ=255) gcgcAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTgc < 1:3120540/69‑1 (MQ=255) gcgcAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTgc < 1:479050/69‑1 (MQ=255) gcgcAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTgc < 1:1816591/69‑1 (MQ=255) gggATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATa < 1:441667/71‑1 (MQ=255) gggATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCACTGATa < 1:2801501/71‑1 (MQ=255) gATGGCGTTACCGCGCAAATTATTATCGTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGa > 1:1182445/1‑71 (MQ=255) gATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGa > 1:4114358/1‑71 (MQ=255) gATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGa > 1:4059716/1‑71 (MQ=255) gATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGa > 1:1224311/1‑71 (MQ=255) aTGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTg > 1:1589783/1‑60 (MQ=255) aTGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGAc > 1:413044/1‑71 (MQ=255) aTGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGAc > 1:2836799/1‑71 (MQ=255) aTGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGAc > 1:2839513/1‑71 (MQ=255) aTGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGAc > 1:794061/1‑71 (MQ=255) aTGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGAc > 1:3843157/1‑71 (MQ=255) aTGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGAc > 1:442333/1‑71 (MQ=255) aTGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGAc > 1:3921525/1‑71 (MQ=255) gCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTg < 1:744252/62‑1 (MQ=255) gCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGc < 1:2566895/70‑1 (MQ=255) cGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAg > 1:2033123/1‑71 (MQ=255) ttACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCttt < 1:1122304/53‑1 (MQ=255) ttACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGAt < 1:2854284/61‑1 (MQ=255) aCCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGgcg < 1:1283544/70‑1 (MQ=255) aCCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGgcg > 1:3461494/1‑70 (MQ=255) aCCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGgcg > 1:445220/1‑70 (MQ=255) aCCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGgcg > 1:4122240/1‑70 (MQ=255) aCCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGgcg < 1:1531111/70‑1 (MQ=255) aCCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGAAgg > 1:4019608/1‑68 (MQ=255) aCCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATAACTTTGCGCTGATAGACGCAGgcg < 1:1063822/70‑1 (MQ=255) aCCGAGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGgcg > 1:3621797/1‑70 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTCCAGCGATATc < 1:781016/38‑1 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTCCAGCGATATc < 1:3938932/38‑1 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTCCAGCGATATc < 1:1900947/38‑1 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTCCAGCGATATc < 1:1523108/38‑1 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCt < 1:381346/46‑1 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCt < 1:2216913/46‑1 (MQ=255) ttattaTCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCt < 1:4095690/46‑1 (MQ=255) attatCCTGAACGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATc < 1:106161/70‑1 (MQ=255) ttatCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTg < 1:2941352/71‑1 (MQ=255) atCCTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGgcg < 1:2131441/55‑1 (MQ=255) cTGACCGTATCCGATGCCTCCAGCGATATCTTTGCGCTGATAGAAGCAGGCGCAGACGGTTATCTGTTGa < 1:1323713/70‑1 (MQ=255) aCCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGa < 1:3374849/71‑1 (MQ=255) aCCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGa < 1:829329/71‑1 (MQ=255) aCCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGa < 1:2754096/71‑1 (MQ=255) tATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGaa < 1:2600374/64‑1 (MQ=255) tCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGtt < 1:55274/52‑1 (MQ=255) cGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGc > 1:1929908/1‑67 (MQ=255) cGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGc > 1:3561514/1‑67 (MQ=255) cGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGc > 1:1478961/1‑67 (MQ=255) cGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGc > 1:2765934/1‑67 (MQ=255) cGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAcc > 1:1107099/1‑71 (MQ=255) cGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAcc > 1:2824864/1‑71 (MQ=255) cGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAcc > 1:2399797/1‑71 (MQ=255) cGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAcc > 1:1537142/1‑71 (MQ=255) cGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAcc > 1:2645725/1‑71 (MQ=255) cGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAcc > 1:2670738/1‑71 (MQ=255) cGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAcc > 1:1386831/1‑71 (MQ=255) cGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAcc > 1:2764047/1‑71 (MQ=255) cGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAcc > 1:1277533/1‑71 (MQ=255) cGATGCCTCCAGCGATATCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGAcc > 1:1254502/1‑71 (MQ=255) gATGCCTCCAGCGATGTCTTTGCGCTGATAGGCGCAGGCGCAGACGGTTATCTGTTGaaa < 1:3686685/60‑1 (MQ=255) gATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGaaa < 1:3748865/60‑1 (MQ=255) tGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGa > 1:3755850/1‑60 (MQ=255) tGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGa > 1:3475957/1‑60 (MQ=255) cTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAg < 1:3418895/71‑1 (MQ=255) tCCAGCGATGTCTTTGCGCTTATAGACGCAGGCGCAGACGGTTATCTGTTGAAAg < 1:155829/55‑1 (MQ=255) tCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAg < 1:954941/55‑1 (MQ=255) tCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAg < 1:1776858/55‑1 (MQ=255) tCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAg < 1:2830863/55‑1 (MQ=255) tCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACa < 1:3716664/58‑1 (MQ=255) cAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAGTAt > 1:3515924/1‑71 (MQ=255) aTGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAGTATTGCTg < 1:2160144/71‑1 (MQ=255) | TTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTATCTGTTGAAAGACAGCGACCCGGAAGTATTGCTG > minE/1418271‑1418402 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |