| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,511,807 | G→A | 21.5% | E359K (GAG→AAG) | pta → | phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,511,807 | 0 | G | A | 21.5% | 201.9 / 50.5 | 93 | E359K (GAG→AAG) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (24/49); new base A (16/4); total (40/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
CACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCT > minE/1511737‑1511875 | caccTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGGCGATCACGAACGTATCg < 1:2234907/71‑1 (MQ=255)caccTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCg < 1:2015572/71‑1 (MQ=255) ctctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAgg < 1:2609009/71‑1 (MQ=255) ctctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGaaa > 1:37585/1‑64 (MQ=255) ctctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAgg < 1:2068363/71‑1 (MQ=255) ctctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAgg < 1:3346144/71‑1 (MQ=255) ctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGaa > 1:3384474/1‑71 (MQ=255) tctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGaa > 1:3891313/1‑70 (MQ=255) tctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGaa > 1:3789430/1‑70 (MQ=255) tctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGaa > 1:2537736/1‑70 (MQ=255) tctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGaa > 1:2888460/1‑70 (MQ=255) tctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGaa > 1:3059493/1‑70 (MQ=255) tctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGaa > 1:591021/1‑70 (MQ=255) aGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGt < 1:2977067/71‑1 (MQ=255) aGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGt < 1:4110546/71‑1 (MQ=255) cTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGc < 1:3859650/71‑1 (MQ=255) cTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGc < 1:959949/71‑1 (MQ=255) tGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAgg < 1:3895385/60‑1 (MQ=255) tGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAgg < 1:3823971/60‑1 (MQ=255) tGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAgg < 1:676648/60‑1 (MQ=255) cAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTaa > 1:3480927/1‑71 (MQ=255) cAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTaa > 1:602488/1‑71 (MQ=255) agagCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAAc < 1:3658272/71‑1 (MQ=255) gagCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGTAAGTTCAgg < 1:500518/56‑1 (MQ=255) gagCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAAc > 1:3314854/1‑70 (MQ=255) cTTCCACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAAt < 1:392195/56‑1 (MQ=255) cTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAAt < 1:3659059/56‑1 (MQ=255) cTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAAt < 1:2816393/56‑1 (MQ=255) cTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAAt < 1:870134/56‑1 (MQ=255) cTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAAt < 1:1229601/56‑1 (MQ=255) cTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAAt < 1:3684138/56‑1 (MQ=255) cTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAAt < 1:1363703/56‑1 (MQ=255) cTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATa < 1:3194744/57‑1 (MQ=255) cTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATa < 1:4050619/57‑1 (MQ=255) cTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:167087/64‑1 (MQ=255) cTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:65227/64‑1 (MQ=255) aaCCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGt > 1:1392410/1‑56 (MQ=255) aaCCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGt > 1:2232129/1‑56 (MQ=255) aaCCTGGAAGTTCCGGTTGACGATCACGAACGTATAAAGAAAGTTCAGGAATACg > 1:905283/1‑55 (MQ=39) tGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATAc < 1:875498/50‑1 (MQ=255) tGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATAc < 1:3002900/50‑1 (MQ=255) gAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGaa > 1:183914/1‑45 (MQ=255) gAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATa < 1:3478315/47‑1 (MQ=255) gAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATa < 1:596310/47‑1 (MQ=255) gAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATa < 1:1731652/47‑1 (MQ=255) gAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATa < 1:1040977/47‑1 (MQ=255) gAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGt > 1:3516006/1‑50 (MQ=255) gAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGt > 1:3109803/1‑50 (MQ=255) gAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGt > 1:2345660/1‑50 (MQ=255) gAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGt > 1:1105590/1‑50 (MQ=255) caaGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATa < 1:2693249/46‑1 (MQ=255) gTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:383253/51‑1 (MQ=255) gTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:1048944/51‑1 (MQ=255) gTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:2329375/51‑1 (MQ=255) gTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:879086/51‑1 (MQ=255) gTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:1409194/51‑1 (MQ=255) gTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:792634/51‑1 (MQ=255) gTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:2549113/51‑1 (MQ=255) gTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:3392248/51‑1 (MQ=255) gTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt < 1:1403522/51‑1 (MQ=255) tCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGa > 1:1240672/1‑71 (MQ=255) tCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGa > 1:1588452/1‑71 (MQ=255) tCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTg > 1:2743706/1‑69 (MQ=255) tCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTg > 1:3294466/1‑69 (MQ=255) tCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTg > 1:4098962/1‑69 (MQ=255) tCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTg > 1:102331/1‑69 (MQ=255) tCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTg > 1:1873543/1‑69 (MQ=255) tCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTg > 1:2001476/1‑69 (MQ=255) tCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTg > 1:2176732/1‑69 (MQ=255) tCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTg > 1:2924531/1‑69 (MQ=255) ggTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCg < 1:2548484/71‑1 (MQ=255) gACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGTCTGGATCGAAtc > 1:1440846/1‑71 (MQ=255) gACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAAtc > 1:1362414/1‑71 (MQ=255) gACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAAtc > 1:1028840/1‑71 (MQ=255) gACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAAtc > 1:884829/1‑71 (MQ=255) gACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAAtc > 1:2285472/1‑71 (MQ=255) gATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATctct < 1:699196/71‑1 (MQ=255) cACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGAc > 1:3445088/1‑71 (MQ=255) tATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACtt < 1:110825/71‑1 (MQ=255) tATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACtt < 1:1861959/71‑1 (MQ=255) tCGAGAAAGTTCAGGAATACGTTGCTAACTACATCATCGCt < 1:639765/41‑1 (MQ=255) tCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCt < 1:3149513/41‑1 (MQ=255) tCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCt < 1:3348148/41‑1 (MQ=255) tCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCt < 1:3432330/41‑1 (MQ=255) tCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCt < 1:2251357/41‑1 (MQ=255) tCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCt < 1:886654/71‑1 (MQ=255) tCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCt < 1:27795/71‑1 (MQ=255) tCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCt < 1:169672/71‑1 (MQ=255) cGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTCACTGCCACTTCt < 1:3699083/70‑1 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGa > 1:2496145/1‑41 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGa > 1:2448623/1‑41 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGaa > 1:3312895/1‑51 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGaa > 1:3786243/1‑51 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGaa > 1:675910/1‑51 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGaa > 1:2021280/1‑51 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAAtct > 1:3373349/1‑54 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTc > 1:3043656/1‑68 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCt > 1:1231562/1‑69 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCt > 1:3397380/1‑69 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCt > 1:1432276/1‑69 (MQ=255) | CACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCT > minE/1511737‑1511875 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |