| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,523,433 | G→T | 29.2% | A78S (GCC→TCC) | flk → | predicted flagella assembly protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,523,433 | 0 | G | T | 29.2% | 85.8 / 23.2 | 41 | A78S (GCC→TCC) | flk | predicted flagella assembly protein |
| Reads supporting (aligned to +/- strand): ref base G (20/9); new base T (9/3); total (29/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
GGTATCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTT > minE/1523373‑1523499 | ggTATCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCa < 1:4072489/71‑1 (MQ=255) tATCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCTTCACTTCCCTGCCGCTGAGCATa < 1:3734627/71‑1 (MQ=255) tCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAAt < 1:1121344/71‑1 (MQ=255) cACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGgc > 1:800784/1‑71 (MQ=255) aCGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGgcg > 1:606130/1‑71 (MQ=255) aCGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGgcg > 1:27827/1‑71 (MQ=255) aCGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGgcg > 1:2377015/1‑71 (MQ=255) atTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACg > 1:311033/1‑71 (MQ=255) atTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACg > 1:765224/1‑71 (MQ=255) atTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACg > 1:3197688/1‑71 (MQ=255) atTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACg > 1:1065587/1‑71 (MQ=255) ggTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCAACGTct > 1:1982076/1‑71 (MQ=255) ggTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCAACGTct > 1:1546216/1‑71 (MQ=255) ggTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCAACGTct > 1:3292627/1‑71 (MQ=255) ggTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTct > 1:1438711/1‑71 (MQ=255) ggTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTct > 1:3840319/1‑71 (MQ=255) gTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTct < 1:2254851/70‑1 (MQ=255) gTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTct < 1:3777420/70‑1 (MQ=255) tCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTctgct < 1:2845640/68‑1 (MQ=255) tCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTctgct < 1:3321152/68‑1 (MQ=255) ggAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCa < 1:2820415/44‑1 (MQ=255) gagaTTCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCa > 1:470470/1‑55 (MQ=255) ttCACCGCTGCTTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCAACg < 1:1615768/54‑1 (MQ=255) cGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGc < 1:4076140/71‑1 (MQ=255) gctTTCGCGTCACTTCCCTTCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCAtt > 1:2779977/1‑71 (MQ=255) ttCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTctg > 1:3928848/1‑46 (MQ=255) ttCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTct > 1:2108983/1‑45 (MQ=255) tCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCaa > 1:1961125/1‑39 (MQ=255) tCACTTCCCTTCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCAt < 1:2974848/61‑1 (MQ=255) cACTTCCCTTCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCAt < 1:1438788/60‑1 (MQ=255) aCTTCCCTTCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTATGCCCGCCAg > 1:553499/1‑71 (MQ=255) ttCCCTTCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGCt > 1:755643/1‑71 (MQ=255) ttCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTc > 1:1267845/1‑59 (MQ=255) tCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTgg > 1:3574750/1‑39 (MQ=255) tCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTgg > 1:4018458/1‑39 (MQ=255) tCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTgg > 1:3225826/1‑39 (MQ=255) tCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTgg > 1:1214576/1‑39 (MQ=255) tCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCTTTc > 1:546662/1‑58 (MQ=255) tCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTc > 1:2881717/1‑58 (MQ=255) ccTTCCGGTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGCttt > 1:815182/1‑70 (MQ=255) ccTTCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGCttt > 1:163029/1‑70 (MQ=255) | GGTATCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTT > minE/1523373‑1523499 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |