| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,533,542 | G→A | 35.4% | P51S (CCT→TCT) | prmB ← | N5‑glutamine methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,533,542 | 0 | G | A | 35.4% | 50.8 / 43.3 | 48 | P51S (CCT→TCT) | prmB | N5‑glutamine methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (21/10); new base A (11/6); total (32/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCG > minE/1533474‑1533607 | tttttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGc < 1:1144480/70‑1 (MQ=255)tttttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGc < 1:4103914/70‑1 (MQ=255)tttttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGc > 1:3302717/1‑70 (MQ=255) tttCGCTGGAGGTCAGACGCGCGGTGCGCATTTCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACaa < 1:713360/70‑1 (MQ=255) tttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACaa < 1:3491441/70‑1 (MQ=255) cAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg > 1:1716070/1‑71 (MQ=255) cAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg > 1:625869/1‑71 (MQ=255) cAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTg > 1:146520/1‑66 (MQ=255) cAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTg > 1:3136695/1‑66 (MQ=255) cAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACgg > 1:406251/1‑71 (MQ=255) cAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACg > 1:2391633/1‑70 (MQ=255) cAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACg > 1:2515951/1‑70 (MQ=255) cgcgGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCAt < 1:1589016/71‑1 (MQ=255) cATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAaca > 1:1730242/1‑44 (MQ=255) cATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGt > 1:2374656/1‑71 (MQ=255) cATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGt > 1:1511101/1‑71 (MQ=255) cATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGt > 1:618988/1‑71 (MQ=255) atCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAaca > 1:1291472/1‑41 (MQ=255) atCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAaca > 1:2059314/1‑41 (MQ=255) atCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAaca > 1:347182/1‑41 (MQ=255) atCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAaca > 1:741374/1‑41 (MQ=255) ttCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCgg > 1:526607/1‑71 (MQ=255) ttCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCgg > 1:1957459/1‑71 (MQ=255) tCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGtt < 1:3405011/65‑1 (MQ=255) ccGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATc < 1:2120385/67‑1 (MQ=255) ccGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATc < 1:2584765/67‑1 (MQ=255) ccGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATc < 1:2738131/67‑1 (MQ=255) ccGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATc < 1:1837882/67‑1 (MQ=255) ccGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCgg < 1:737961/69‑1 (MQ=255) ccGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCgg < 1:1245170/69‑1 (MQ=255) ccGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCgg < 1:749709/69‑1 (MQ=255) tCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGAcc > 1:71184/1‑71 (MQ=255) tCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGAcc > 1:1619163/1‑71 (MQ=255) tCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGAcc > 1:1592452/1‑71 (MQ=255) cAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCAt > 1:2291991/1‑44 (MQ=255) cAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg > 1:221454/1‑70 (MQ=255) cAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg < 1:1192604/70‑1 (MQ=255) ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTAc > 1:1962272/1‑69 (MQ=255) gaagagCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTAc < 1:780222/62‑1 (MQ=255) tAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAtt < 1:2655619/71‑1 (MQ=255) agagCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAttt > 1:51418/1‑71 (MQ=255) agagCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAttt > 1:1147796/1‑71 (MQ=255) agagCGAAGACAACACCAGCTGTACGGCTTCATcc > 1:2571183/1‑35 (MQ=255) agagCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTa > 1:1476817/1‑50 (MQ=255) agagCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAttt > 1:1371595/1‑71 (MQ=255) agagCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAtt > 1:2246957/1‑70 (MQ=255) cGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCcg > 1:337362/1‑71 (MQ=255) cGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCcg > 1:1220147/1‑71 (MQ=255) | TTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCG > minE/1533474‑1533607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |