| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,544,443 | G→A | 9.7% | L427F (CTT→TTT) | yfcY ← | beta‑ketoacyl‑CoA thiolase, anaerobic, subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,544,443 | 0 | G | A | 9.7% | 188.5 / 8.3 | 62 | L427F (CTT→TTT) | yfcY | beta‑ketoacyl‑CoA thiolase, anaerobic, subunit |
| Reads supporting (aligned to +/- strand): ref base G (40/16); new base A (3/3); total (43/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTG > minE/1544373‑1544512 | cAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAg > 1:526835/1‑71 (MQ=255)cAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAg > 1:2879699/1‑71 (MQ=255)cAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAg > 1:1490608/1‑71 (MQ=255)cAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAg > 1:1971234/1‑71 (MQ=255) aCGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAAccc < 1:406794/71‑1 (MQ=255) gAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCAc > 1:373839/1‑71 (MQ=255) gAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCAc > 1:2776007/1‑71 (MQ=255) cATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGccc < 1:600432/66‑1 (MQ=255) cATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGccc < 1:2644965/66‑1 (MQ=255) cATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGccc < 1:1850001/66‑1 (MQ=255) tAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGgcag > 1:933054/1‑71 (MQ=255) aaGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGgcag < 1:716989/70‑1 (MQ=255) aaGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGgcag < 1:2615948/70‑1 (MQ=255) aaGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGgcag < 1:2559764/70‑1 (MQ=255) aaGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAACCCACCGgcag > 1:897164/1‑70 (MQ=255) aaaCGCTGATGTCAATTCCATTAATCCGCCTCCAGAACCATTGCCGCGCCAAAccc < 1:3456386/56‑1 (MQ=255) aaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCg > 1:1097775/1‑71 (MQ=255) aaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCg > 1:2556445/1‑71 (MQ=255) cGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGt < 1:105428/71‑1 (MQ=255) cGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGt < 1:1077301/71‑1 (MQ=255) tGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGt < 1:1699040/68‑1 (MQ=255) aTGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACt > 1:3085767/1‑70 (MQ=255) aTGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACt > 1:2883741/1‑70 (MQ=255) tGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTaa < 1:30986/71‑1 (MQ=255) gTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTaaa > 1:884335/1‑71 (MQ=255) gTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTaaa > 1:251226/1‑71 (MQ=255) gTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTaaa > 1:3057932/1‑71 (MQ=255) gTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTaaa > 1:3365250/1‑71 (MQ=255) gTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTaaa > 1:3305340/1‑71 (MQ=255) aTTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAAcc > 1:3374534/1‑70 (MQ=255) ttCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAAccc < 1:2399327/41‑1 (MQ=255) tCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAt > 1:3801029/1‑71 (MQ=255) tCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAt > 1:691016/1‑71 (MQ=255) tCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAAt > 1:3305740/1‑71 (MQ=255) cATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATc > 1:2374563/1‑70 (MQ=255) ttattCCGCCTCCAGAACCATTGCCGCGCCAAGcc > 1:2692643/1‑35 (MQ=255) ttattCCGCCTCCAGAACCATTGCCGCGCCAAGcc > 1:4102959/1‑35 (MQ=255) ttattCCGCCTCCAGAACCATTGCCGCGCCAAGcc > 1:10997/1‑35 (MQ=255) ttattCCGCCTCCAGAACCATTGCCGCGCCAAGcc > 1:3022493/1‑35 (MQ=255) attCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCAccg > 1:3197573/1‑71 (MQ=255) attCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCAccg > 1:2751368/1‑71 (MQ=255) ttCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGgcagca > 1:27634/1‑43 (MQ=255) ttCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGgcagca > 1:172161/1‑43 (MQ=255) cGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACcgccgc < 1:511555/71‑1 (MQ=255) cGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACcgccgc < 1:3965515/71‑1 (MQ=255) cAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGc < 1:2826270/71‑1 (MQ=255) gAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGa > 1:2224652/1‑65 (MQ=255) gAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCg > 1:1158149/1‑70 (MQ=255) gAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCg > 1:2334240/1‑70 (MQ=255) gAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCg > 1:2016463/1‑70 (MQ=255) gAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCg > 1:2113162/1‑70 (MQ=255) gAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCg > 1:3124705/1‑70 (MQ=255) cATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGtt < 1:4076791/71‑1 (MQ=255) cATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGtt < 1:4122747/71‑1 (MQ=255) gcgcCAAACCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCaa > 1:3640326/1‑71 (MQ=255) gcgcCAAACCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCaa > 1:439662/1‑71 (MQ=255) cgcCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCaa < 1:2171322/70‑1 (MQ=255) ccAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATg > 1:1958386/1‑70 (MQ=255) ccAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGt > 1:47144/1‑71 (MQ=255) ccAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGt > 1:1752510/1‑71 (MQ=255) ccAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGt > 1:1218858/1‑71 (MQ=255) ccAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGt > 1:2831239/1‑71 (MQ=255) ccAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGt > 1:1719324/1‑71 (MQ=255) ccAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGt > 1:1894017/1‑71 (MQ=255) aGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTg > 1:601400/1‑71 (MQ=255) aGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTg > 1:898419/1‑71 (MQ=255) | CAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTG > minE/1544373‑1544512 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |