| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 199,135 | T→A | 33.8% | S207C (AGC→TGC) | metQ ← | DL‑methionine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 199,135 | 0 | T | A | 33.8% | 73.7 / 66.0 | 68 | S207C (AGC→TGC) | metQ | DL‑methionine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (17/28); new base A (13/10); total (30/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01 | |||||||||||
GTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCAGTTGCG > minE/199067‑199200 | gTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggctgg < 1:3744343/71‑1 (MQ=255)gTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggctgg < 1:3801964/71‑1 (MQ=255)gTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggctgg < 1:2955412/71‑1 (MQ=255) ttACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCa > 1:3566682/1‑71 (MQ=255) tacgtacgGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCa > 1:3267759/1‑70 (MQ=255) tacgtacgGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTAAGGCCAATCTGGCTGGCa > 1:2275250/1‑70 (MQ=255) cgtacgGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATa < 1:530381/70‑1 (MQ=255) cgtacgGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATa < 1:4011808/70‑1 (MQ=255) cgGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggtgg < 1:3775403/71‑1 (MQ=255) cgGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCAt > 1:3031980/1‑65 (MQ=255) ggggACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggtggt < 1:2294344/71‑1 (MQ=255) ggggACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAggtggt < 1:2972531/71‑1 (MQ=255) ggACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGTAGTCAGGCCAATCTGGCAGGCATa < 1:2928344/63‑1 (MQ=255) ggACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATa < 1:2063653/63‑1 (MQ=255) gACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCAt < 1:2447980/61‑1 (MQ=255) gACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCAt < 1:569438/61‑1 (MQ=255) gACCCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCa > 1:3058600/1‑60 (MQ=255) aCTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAgg > 1:3630283/1‑63 (MQ=255) aCTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAgg > 1:1922939/1‑63 (MQ=255) ctTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCAt < 1:187568/57‑1 (MQ=255) ctTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCAt < 1:2069553/57‑1 (MQ=255) ctTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCAt < 1:261707/57‑1 (MQ=255) tttATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggctg > 1:3943519/1‑52 (MQ=255) tttATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggctg > 1:1419900/1‑52 (MQ=255) tttATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggctg > 1:2601410/1‑52 (MQ=255) tttATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggct < 1:2434338/51‑1 (MQ=255) tttATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGAt < 1:2959256/69‑1 (MQ=255) tttATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGAt < 1:1416274/69‑1 (MQ=255) tttATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGAt < 1:348024/69‑1 (MQ=255) tATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAGGTGGTATTGATAACt > 1:1099104/1‑71 (MQ=255) tATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAGGTGGTATTGATAACt > 1:3733224/1‑71 (MQ=255) tATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAGGTGGTATTGATAACt > 1:1640102/1‑71 (MQ=255) aTCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATa < 1:1576679/67‑1 (MQ=255) aTCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTg > 1:477322/1‑71 (MQ=255) aTCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTg > 1:3484109/1‑71 (MQ=255) aTCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTg > 1:3486341/1‑71 (MQ=255) tCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTggcaggca > 1:1310819/1‑51 (MQ=255) ttCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACAGc > 1:3300623/1‑69 (MQ=255) taTACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCAt < 1:1506954/40‑1 (MQ=255) gATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCAt < 1:1367774/41‑1 (MQ=255) gATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCAt < 1:2210922/41‑1 (MQ=255) gATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCAt < 1:2765527/41‑1 (MQ=255) gATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCAt < 1:2339535/41‑1 (MQ=255) gATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCAt < 1:2917865/41‑1 (MQ=255) tACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATa > 1:3662187/1‑40 (MQ=255) tACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAg > 1:2459336/1‑41 (MQ=255) tACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAg > 1:2902255/1‑41 (MQ=255) tACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAg > 1:3510939/1‑41 (MQ=255) tACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAg > 1:663136/1‑41 (MQ=255) tACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCAGGCATAg > 1:104683/1‑41 (MQ=255) aCCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCag < 1:1219190/60‑1 (MQ=255) aCCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCag < 1:2149634/60‑1 (MQ=255) aCCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCag < 1:337033/60‑1 (MQ=255) ccGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTgcgc < 1:1762485/71‑1 (MQ=255) cGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCagag > 1:2899734/1‑60 (MQ=255) cGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCagag > 1:2037236/1‑60 (MQ=255) cGTCTTTCGCC‑GAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCagag > 1:2162621/1‑59 (MQ=255) tttCGCCGGAGTCAGGCCAATCTGGCTGGCATAggtggt < 1:1311553/39‑1 (MQ=255) tttCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCa > 1:2244827/1‑53 (MQ=255) tttCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGcgtcgt > 1:3050286/1‑71 (MQ=255) tttCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGcgtcgt > 1:278506/1‑71 (MQ=255) tttCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGcgtcgt > 1:814759/1‑71 (MQ=255) tttCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGcgtcgt > 1:1758979/1‑71 (MQ=255) gCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCa < 1:1445629/70‑1 (MQ=255) gCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCa < 1:1250369/70‑1 (MQ=255) cGGAGTCAGGCCAATCTGGCAGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCagag < 1:3048637/71‑1 (MQ=255) gTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAAcg > 1:4081806/1‑71 (MQ=255) tCAGGCCTATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCg < 1:594350/47‑1 (MQ=255) tCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCg < 1:665986/47‑1 (MQ=255) ggCCAATCTGGCTGGCATAGGTTGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGc < 1:1149339/71‑1 (MQ=255) ggCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGc < 1:999962/71‑1 (MQ=255) ggCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGc < 1:1437369/71‑1 (MQ=255) ggCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGc < 1:2592113/71‑1 (MQ=255) aaTCTGGCAGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGAGTCGTCCAGAGAACGCGGCAGtt > 1:3315777/1‑71 (MQ=255) aTCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGc < 1:1441010/66‑1 (MQ=255) aTCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCAGTTg < 1:3750556/71‑1 (MQ=255) ctggctggCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCAGTTGCg > 1:556940/1‑71 (MQ=255) | GTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCAGTTGCG > minE/199067‑199200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |