BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,697,632	A→G	22.4%	Y195C (TAT→TGT)	yfhR →	predicted peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,697,632	0	A	G	22.4%	196.5 / 57.8	98	Y195C (TAT→TGT)	yfhR	predicted peptidase
Reads supporting (aligned to +/- strand): ref base A (36/40); new base G (7/15); total (43/55)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

ATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAGCGGC  >  minE/1697566‑1697696

aTGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCa                                                              <  1:42271/71‑1 (MQ=255)

 tGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCaa                                                             >  1:3688540/1‑71 (MQ=255)

  gTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAAc                                                            >  1:2903344/1‑71 (MQ=255)

  gTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAAc                                                            >  1:3727518/1‑71 (MQ=255)

     aTTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCAt                                                         >  1:3387699/1‑71 (MQ=255)

      ttGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCAt                                                         <  1:1984676/70‑1 (MQ=255)

      ttGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCAt                                                         <  1:2837985/70‑1 (MQ=255)

      ttGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCAt                                                         <  1:1396552/70‑1 (MQ=255)

      ttGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATc                                                        <  1:893631/71‑1 (MQ=255)

       tGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATc                                                        <  1:4124520/70‑1 (MQ=255)

        ggTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGc                                                      >  1:1424233/1‑71 (MQ=255)

                    cGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGa                                           >  1:1099291/1‑70 (MQ=255)

                    cGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGAt                                          >  1:2116946/1‑71 (MQ=255)

                    cGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGAt                                          >  1:537035/1‑71 (MQ=255)

                     gTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATc                                         <  1:4011440/71‑1 (MQ=255)

                     gTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATc                                         <  1:1734684/71‑1 (MQ=255)

                     gTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATc                                         <  1:1729558/71‑1 (MQ=255)

                       gAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATg                                            <  1:3509358/66‑1 (MQ=255)

                        aaGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATg                                            <  1:1481056/65‑1 (MQ=255)

                          ggCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCgg                                    <  1:131624/71‑1 (MQ=255)

                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAAt                                             <  1:4000483/61‑1 (MQ=255)

                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAAt                                             <  1:1016813/61‑1 (MQ=255)

                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAAt                                             <  1:1652780/61‑1 (MQ=255)

                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAAt                                             <  1:323440/61‑1 (MQ=255)

                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAAt                                             <  1:3251041/61‑1 (MQ=255)

                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAAt                                             <  1:4044812/61‑1 (MQ=255)

                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGc                                   <  1:2727542/71‑1 (MQ=255)

                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGc                                   <  1:21714/71‑1 (MQ=255)

                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGc                                   <  1:2100879/71‑1 (MQ=255)

                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGc                                   <  1:894413/71‑1 (MQ=255)

                           gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGc                                   <  1:1277760/71‑1 (MQ=255)

                             aTACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAg                                 >  1:503493/1‑71 (MQ=255)

                             aTACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAg                                 >  1:85533/1‑71 (MQ=255)

                              tACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaaccaa                                               <  1:3680437/56‑1 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTa                                                                  >  1:1397790/1‑35 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTa                                                                  >  1:2240224/1‑35 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTa                                                                  >  1:4066049/1‑35 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCa                                                          >  1:2512264/1‑43 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAAt                                             >  1:1033006/1‑56 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAAt                                             >  1:3188585/1‑56 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAAt                                             >  1:1688976/1‑56 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAAt                                             >  1:3644604/1‑56 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAAt                                             >  1:2731556/1‑56 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGAt                                          >  1:2311419/1‑59 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGAt                                          >  1:2731915/1‑59 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGAt                                          >  1:3594457/1‑59 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAg                                 >  1:3232558/1‑68 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTgg                              >  1:3363968/1‑71 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTgg                              >  1:1281/1‑71 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTgg                              >  1:1512266/1‑71 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTgg                              >  1:225991/1‑71 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTgg                              >  1:3631077/1‑71 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTg                               <  1:2425206/70‑1 (MQ=255)

                                cGTGCGGTGATCCTCGACTCCACATTTGCATCTTa                                                                  >  1:3469951/1‑35 (MQ=255)

                                  tGCGGTGATCCTCGACTCCACATTTGCCTCTTGTTCAACCATCGCCAACCAAAt                                             <  1:1694980/54‑1 (MQ=255)

                                  tGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGc                                   <  1:2884701/64‑1 (MQ=255)

                                  tGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGc                                   <  1:2973276/64‑1 (MQ=255)

                                  tGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGc                                   <  1:3860802/64‑1 (MQ=255)

                                  tGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGc                                   <  1:545346/64‑1 (MQ=255)

                                  tGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGc                                   <  1:2518387/64‑1 (MQ=255)

                                  tGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacca                                                <  1:3098936/51‑1 (MQ=255)

                                  tGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacca                                                <  1:2252205/51‑1 (MQ=255)

                                  tGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacca                                                <  1:1924158/51‑1 (MQ=255)

                                    cGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCtac                          >  1:3971781/1‑71 (MQ=255)

                                     ggTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATc                                         <  1:1028303/55‑1 (MQ=255)

                                         aTCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCtacttact                     >  1:2665399/1‑71 (MQ=255)

                                         aTCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCtacttact                     >  1:865471/1‑71 (MQ=255)

                                         aTCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCtacttact                     >  1:3137949/1‑71 (MQ=255)

                                           ccTCGACTCCACATTTGCTTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTacttactt                    >  1:2917546/1‑70 (MQ=255)

                                           ccTCGACTCCACATTTGCTTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTacttactt                    >  1:3339243/1‑70 (MQ=255)

                                           ccTCGACTCCACATTTGCTTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTacttactt                    >  1:3150152/1‑70 (MQ=255)

                                           ccTCGACTCCACATTTGCTTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTacttactt                    >  1:1299697/1‑70 (MQ=255)

                                           ccTCGACTCCACATTTGCTTCTTATGCAACCATCGCCAACCAAATGATACCCGGCAGTGGCTacttactt                    >  1:214317/1‑70 (MQ=255)

                                           ccTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTacttactt                    >  1:2527882/1‑70 (MQ=255)

                                            cTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGc                                   <  1:3786783/54‑1 (MQ=255)

                                              cGACTCCACATTTGCCTCTTGTGCAACCATCGccaa                                                   >  1:1065133/1‑36 (MQ=255)

                                              cGACTCCACATTTGCCTCTTGTGCAACCATCGccaa                                                   >  1:1600391/1‑36 (MQ=255)

                                               gACTCCACATTTGCCTCTTATGCAACCCTCGCCAACCAAATGATCCCCTGc                                   <  1:1408790/51‑1 (MQ=255)

                                                  tCCACATTTGCCTCTTATGCAACCATCGccaacca                                                <  1:10146/35‑1 (MQ=255)

                                                  tCCACATTTGCCTCTTATGCAACCATCGccaacca                                                <  1:2212269/35‑1 (MQ=255)

                                                  tCCACATTTGCCTCTTATGCAACCATCGccaacca                                                <  1:3028516/35‑1 (MQ=255)

                                                    cacaTTTGCCTCTTATGCAACCATCGCCAACCAAATGATc                                         >  1:191682/1‑40 (MQ=255)

                                                    cacaTTTGCCTCTTATGCAACCATCGCCAACCAAATGATc                                         >  1:2597403/1‑40 (MQ=255)

                                                      caTTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCt                            <  1:3496962/51‑1 (MQ=255)

                                                      caTTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCt                            <  1:197317/51‑1 (MQ=255)

                                                      caTTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCt                            <  1:2583265/51‑1 (MQ=255)

                                                      caTTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCt                            <  1:3028390/51‑1 (MQ=255)

                                                      caTTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCt                            <  1:1478111/51‑1 (MQ=255)

                                                      caTTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtgatga               <  1:713776/64‑1 (MQ=255)

                                                      caTTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTAc        <  1:293514/71‑1 (MQ=255)

                                                      caTTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTAc        <  1:4081525/71‑1 (MQ=255)

                                                        tttGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCtactt                        <  1:2070684/53‑1 (MQ=255)

                                                          tGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAgcg    >  1:1765460/1‑71 (MQ=255)

                                                            ccTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTAc        <  1:1366570/65‑1 (MQ=255)

                                                             ctctTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAgcggc  <  1:3241364/70‑1 (MQ=255)

                                                               ctTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtg                   <  1:1094675/51‑1 (MQ=255)

                                                                ttATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGt           >  1:707579/1‑58 (MQ=255)

                                                                ttATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAgc     <  1:125492/64‑1 (MQ=255)

                                                                ttATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAgc     <  1:2421785/64‑1 (MQ=255)

                                                                ttATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAgc     <  1:218160/64‑1 (MQ=255)

ATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAGCGGC  >  minE/1697566‑1697696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`