| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,747,876 | G→A | 28.6% | S44L (TCA→TTA) | yfiE ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,747,876 | 0 | G | A | 28.6% | 77.5 / 34.9 | 49 | S44L (TCA→TTA) | yfiE | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (8/27); new base A (6/8); total (14/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTA > minE/1747809‑1747926 | cgtcagcaACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:4029465/67‑1 (MQ=255) cagcaACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:385568/67‑1 (MQ=255) cagcaACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACt < 1:1074357/71‑1 (MQ=255) ttttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:880906/60‑1 (MQ=255) ttttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:546619/60‑1 (MQ=255) ttttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:3810701/60‑1 (MQ=255) ttttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:3102781/60‑1 (MQ=255) gTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGctgctg < 1:3084331/65‑1 (MQ=255) gTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGctgctg < 1:580049/65‑1 (MQ=255) aGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGaaa < 1:2808832/71‑1 (MQ=255) aGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGaa < 1:231899/70‑1 (MQ=255) aGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAAATCCTGCTCAAGCTGCTGAATATGaaa < 1:1942029/71‑1 (MQ=255) acaTCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:1882727/71‑1 (MQ=255) acaTCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:2777339/71‑1 (MQ=255) acaTCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:817752/71‑1 (MQ=255) aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:3108976/69‑1 (MQ=255) aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:1289111/69‑1 (MQ=255) aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:1965817/69‑1 (MQ=255) aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:3970946/69‑1 (MQ=255) ggCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGa < 1:3955729/70‑1 (MQ=255) ggCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGa < 1:1855066/70‑1 (MQ=255) ggCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGa < 1:1416738/70‑1 (MQ=255) ggCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGa < 1:3239390/70‑1 (MQ=255) ggCCAATTTACTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGa < 1:952236/70‑1 (MQ=255) gCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGa < 1:3709257/69‑1 (MQ=255) ccAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtg < 1:2457986/71‑1 (MQ=255) cAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATg < 1:2650748/52‑1 (MQ=255) cAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATg < 1:1487964/52‑1 (MQ=255) aTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg > 1:430156/1‑70 (MQ=255) aTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg > 1:1623502/1‑70 (MQ=255) aTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg > 1:3108859/1‑70 (MQ=255) aTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg > 1:624473/1‑70 (MQ=255) tttCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTa > 1:1034639/1‑70 (MQ=255) tttCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTa > 1:1005428/1‑70 (MQ=255) ttCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACTGTCGAt > 1:2262723/1‑63 (MQ=255) ttCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGAt > 1:3890850/1‑63 (MQ=255) ttCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGAt > 1:2913126/1‑63 (MQ=255) ttCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGAt > 1:207916/1‑63 (MQ=255) ttCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGAt > 1:1567877/1‑63 (MQ=255) ttCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGAt > 1:1082828/1‑63 (MQ=255) tAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCAc > 1:304682/1‑48 (MQ=255) tAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCAc > 1:1212474/1‑48 (MQ=255) aCTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:3454320/52‑1 (MQ=255) aCTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:3010383/52‑1 (MQ=255) aCTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:1151017/52‑1 (MQ=255) tGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:3972837/37‑1 (MQ=255) tGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:2186239/37‑1 (MQ=255) tGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:4058469/37‑1 (MQ=255) tGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:2181401/37‑1 (MQ=255) | CGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTA > minE/1747809‑1747926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |