| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,798,695 | A→G | 37.7% | Q239R (CAA→CGA) | proV → | glycine betaine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,798,695 | 0 | A | G | 37.7% | 49.4 / 66.8 | 61 | Q239R (CAA→CGA) | proV | glycine betaine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (20/18); new base G (11/12); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01 | |||||||||||
GCACCATTGTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCG > minE/1798626‑1798764 | gcACCATTGTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCaa < 1:2574989/71‑1 (MQ=255) aTTGTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCGAAATgg > 1:1119769/1‑71 (MQ=255) ttGTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCGAAATGGt > 1:3822855/1‑71 (MQ=255) ttGTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCGAAATGGt > 1:2925922/1‑71 (MQ=255) ttGTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCGAAATGGt > 1:2391993/1‑71 (MQ=255) gTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCGAAATGGTg < 1:3839602/70‑1 (MQ=255) gTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCGAAATGGTg < 1:3043754/70‑1 (MQ=255) gTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCGAAATGGTg < 1:3175036/70‑1 (MQ=255) gTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCGAAATGGTg > 1:3209986/1‑70 (MQ=255) gTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTg < 1:2323083/70‑1 (MQ=255) gTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTg < 1:3578013/70‑1 (MQ=255) gTCTTTATTTCCCACGATCTTGAAGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTg < 1:542002/70‑1 (MQ=255) tCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGa < 1:2205607/70‑1 (MQ=255) tATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAg > 1:433658/1‑68 (MQ=255) tATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAg > 1:2624293/1‑68 (MQ=255) tATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAg > 1:2272803/1‑68 (MQ=255) tATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAg > 1:3956853/1‑68 (MQ=255) tATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAg > 1:3397636/1‑68 (MQ=255) tATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAg > 1:1568946/1‑68 (MQ=255) tATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAg > 1:835980/1‑68 (MQ=255) aTTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCGAAATGGTGAAGTgg < 1:3684314/70‑1 (MQ=255) gatgaAGCCATGCGTATTGGCGACCGAATTGCCATTATGCGAAATGGTGAAGTGGTACAGGTCGGca < 1:2966346/67‑1 (MQ=255) gCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGa < 1:1723340/71‑1 (MQ=255) gCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGa < 1:2944114/71‑1 (MQ=255) tGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAAtt < 1:1579275/71‑1 (MQ=255) tGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAAtt < 1:63166/71‑1 (MQ=255) gCGTATTGGCGACCGAATTGCCATTATGCGAAATGGTGAAGTgg < 1:3030607/44‑1 (MQ=255) gCGTATTGGCGACCGAATTGCCATTATGCGAAATGGTGAAGTgg < 1:3093841/44‑1 (MQ=255) cGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATtct > 1:3810864/1‑71 (MQ=255) cGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATtct > 1:1323748/1‑71 (MQ=255) cGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATtct > 1:1132715/1‑71 (MQ=255) gTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATtct > 1:2535019/1‑70 (MQ=255) tATTGGCGACCGAATTGCCATTATGCAAAATGGTg < 1:955453/35‑1 (MQ=255) ttGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGaaa > 1:2555851/1‑63 (MQ=255) ttGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGaaa > 1:1991582/1‑63 (MQ=255) ttGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCaa < 1:3325108/70‑1 (MQ=255) ggCGACCGAATTGCCATTATGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCaataa > 1:339864/1‑71 (MQ=255) ggCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCaata < 1:3933412/70‑1 (MQ=255) gCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTGCAGGTCGGCACACCGGATGAAATTCTCaataa < 1:1204460/70‑1 (MQ=255) gCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCaataa < 1:1986731/70‑1 (MQ=255) gCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCaataa < 1:1982355/70‑1 (MQ=255) gACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCaataat > 1:2884277/1‑69 (MQ=255) aaTTGCCATTATGCGAAATTGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGa < 1:238177/71‑1 (MQ=255) aTTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGaa > 1:1708158/1‑71 (MQ=255) aTTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGaa > 1:874873/1‑71 (MQ=255) ttGCCATTATGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAAt < 1:3559175/71‑1 (MQ=255) ttGCCATTATGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAAt < 1:369809/71‑1 (MQ=255) cATTATGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGAtt < 1:3856490/71‑1 (MQ=255) aTTATGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTa > 1:2341673/1‑71 (MQ=255) ttATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAAtt < 1:545374/47‑1 (MQ=255) aTGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGa > 1:3016873/1‑65 (MQ=255) aTGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGa > 1:3865516/1‑65 (MQ=255) aTGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGa > 1:3220603/1‑65 (MQ=255) aTGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGa > 1:2182641/1‑65 (MQ=255) aTGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATg < 1:386021/70‑1 (MQ=255) aTGCAAAATGGTGAAGTGTTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATg < 1:1174161/64‑1 (MQ=255) aTGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATg < 1:1096281/64‑1 (MQ=255) aTGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGt > 1:3254768/1‑71 (MQ=255) aTGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGt > 1:1074315/1‑71 (MQ=255) tGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATg < 1:3908437/63‑1 (MQ=255) cAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTcct > 1:1124351/1‑70 (MQ=255) cAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCg > 1:1275714/1‑71 (MQ=255) | GCACCATTGTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGATTATGTCCG > minE/1798626‑1798764 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |