| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,807,377 | T→C | 25.2% | intergenic (+58/+6) | emrB → / ← luxS | multidrug efflux system protein/S‑ribosylhomocysteinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,807,377 | 0 | T | C | 25.2% | 177.2 / 67.2 | 99 | intergenic (+58/+6) | emrB/luxS | multidrug efflux system protein/S‑ribosylhomocysteinase |
| Reads supporting (aligned to +/- strand): ref base T (32/42); new base C (7/18); total (39/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 104 total aligned reads displayed.
TGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCG > minE/1807310‑1807445 | tGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACt < 1:302199/71‑1 (MQ=255) gcgcACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACt > 1:3311785/1‑70 (MQ=255) aCTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTa < 1:4074257/71‑1 (MQ=255) cTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGAc > 1:3372775/1‑68 (MQ=255) cTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGAc > 1:3707541/1‑68 (MQ=255) aaGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa > 1:4090686/1‑61 (MQ=255) aaGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa > 1:3922716/1‑61 (MQ=255) aaGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAt > 1:2152591/1‑71 (MQ=255) aGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtg > 1:2457938/1‑71 (MQ=255) tACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTa < 1:2073249/65‑1 (MQ=255) aCAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAt > 1:928801/1‑67 (MQ=255) aCAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAt > 1:2413393/1‑67 (MQ=255) aCAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAt > 1:2222813/1‑67 (MQ=255) aCAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAt > 1:118882/1‑67 (MQ=255) aCAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGCTTACTGACTAGAt > 1:303842/1‑67 (MQ=255) cAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGc < 1:2450463/70‑1 (MQ=255) cAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGc < 1:2160350/70‑1 (MQ=255) cAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGc < 1:3280643/70‑1 (MQ=255) aCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGt < 1:2123136/71‑1 (MQ=255) ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGc < 1:2720713/71‑1 (MQ=255) ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGc < 1:1510228/71‑1 (MQ=255) cAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGc < 1:3546465/70‑1 (MQ=255) aGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCaa > 1:1529015/1‑71 (MQ=255) aGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCaa > 1:358252/1‑71 (MQ=255) aGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCaa > 1:807199/1‑71 (MQ=255) gTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtgtg < 1:2993018/58‑1 (MQ=255) gTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtgtg < 1:429526/58‑1 (MQ=255) ttCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACt < 1:1517368/71‑1 (MQ=255) tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa > 1:2852111/1‑43 (MQ=255) tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa > 1:3731951/1‑43 (MQ=255) tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa > 1:1117152/1‑43 (MQ=255) tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa > 1:1797104/1‑43 (MQ=255) tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa < 1:1130188/58‑1 (MQ=255) tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa < 1:1198723/58‑1 (MQ=255) tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa < 1:609597/58‑1 (MQ=255) tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGCGCa > 1:1488340/1‑58 (MQ=255) cATTTGAAGTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCaa > 1:2565937/1‑67 (MQ=255) cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc > 1:1772486/1‑71 (MQ=255) cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc > 1:3387678/1‑71 (MQ=255) cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc > 1:291938/1‑71 (MQ=255) cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc > 1:1182642/1‑71 (MQ=255) cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc > 1:2331305/1‑71 (MQ=255) cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc > 1:2926109/1‑71 (MQ=255) cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc > 1:783695/1‑71 (MQ=255) cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTtc > 1:712745/1‑71 (MQ=255) tttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa < 1:523508/55‑1 (MQ=255) tttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa < 1:310935/55‑1 (MQ=255) tttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa < 1:276699/55‑1 (MQ=255) tttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtctc < 1:1188760/71‑1 (MQ=255) tttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtctc < 1:129797/71‑1 (MQ=255) tGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCtt > 1:2229973/1‑71 (MQ=255) gAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCtt < 1:1091760/70‑1 (MQ=255) aCTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCg > 1:1544082/1‑71 (MQ=255) cTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAg < 1:163367/42‑1 (MQ=255) cTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAg < 1:3735591/42‑1 (MQ=255) cTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAg < 1:1952386/42‑1 (MQ=255) cTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAg < 1:3000402/42‑1 (MQ=255) ggCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCgg > 1:1806525/1‑69 (MQ=255) gCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc < 1:1527406/69‑1 (MQ=255) gCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc < 1:4055965/69‑1 (MQ=255) gCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc < 1:3096161/69‑1 (MQ=255) gCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc < 1:1693412/69‑1 (MQ=255) gCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAg > 1:2137279/1‑71 (MQ=255) gCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAg > 1:2096926/1‑71 (MQ=255) tttCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGcc < 1:3756337/70‑1 (MQ=255) tttCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGcc < 1:2822192/70‑1 (MQ=255) tttCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGcc < 1:2869375/70‑1 (MQ=255) ttCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAg > 1:2403418/1‑71 (MQ=255) ttCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAg > 1:656504/1‑71 (MQ=255) cAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGt < 1:646741/70‑1 (MQ=255) tattaattGTGAAGATAGTTTACTGACTAGATGTGCa < 1:1833321/36‑1 (MQ=255) attaattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTctct < 1:2154667/53‑1 (MQ=255) attaattGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGc < 1:3102505/44‑1 (MQ=255) ttaattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTG‑AACTTCTCTTTCGGCAGTGCCAGttcttc < 1:2481084/71‑1 (MQ=255) aattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttcttc < 1:3260968/70‑1 (MQ=255) aattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttcttc < 1:1396952/70‑1 (MQ=255) aattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCACttcttc < 1:3366788/70‑1 (MQ=255) attGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttcttc < 1:1318322/69‑1 (MQ=255) attGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttcttc < 1:1659450/69‑1 (MQ=255) attGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCACttcttc < 1:3371565/69‑1 (MQ=255) gAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc < 1:2625602/35‑1 (MQ=255) gAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc < 1:636801/35‑1 (MQ=255) gAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc < 1:290636/35‑1 (MQ=255) gAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc < 1:3846460/35‑1 (MQ=255) gAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc < 1:1297490/35‑1 (MQ=255) gAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc < 1:1901661/35‑1 (MQ=255) aaGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTg < 1:3822649/70‑1 (MQ=255) aaGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTg < 1:892506/70‑1 (MQ=255) aaGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTg < 1:2555205/70‑1 (MQ=255) aGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGtt < 1:1811646/71‑1 (MQ=255) aGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGtt < 1:2583622/71‑1 (MQ=255) aGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGtt < 1:2030352/71‑1 (MQ=255) tAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGAt < 1:2895173/71‑1 (MQ=255) aGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATg > 1:921258/1‑71 (MQ=255) gTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGc > 1:479090/1‑71 (MQ=255) gTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGc > 1:2763003/1‑71 (MQ=255) tttACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCg < 1:1945608/71‑1 (MQ=255) tttACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCg < 1:3199350/71‑1 (MQ=255) tttACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCg < 1:2989850/71‑1 (MQ=255) ttACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCg > 1:2664645/1‑70 (MQ=255) | TGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCG > minE/1807310‑1807445 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |