| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,827,932 | C→T | 33.8% | A392V (GCG→GTG) | mutS → | methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,827,932 | 0 | C | T | 33.8% | 76.8 / 59.3 | 65 | A392V (GCG→GTG) | mutS | methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base C (14/29); new base T (15/7); total (29/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACAC > minE/1827872‑1827998 | tCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTg < 1:2577564/69‑1 (MQ=255) ccAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTgag < 1:3827069/70‑1 (MQ=255) ccAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTg < 1:853388/68‑1 (MQ=255) cAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaa < 1:2919220/71‑1 (MQ=255) gCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaaga < 1:2980277/71‑1 (MQ=255) gCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaaga < 1:2350754/71‑1 (MQ=255) gCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaaga < 1:323222/71‑1 (MQ=255) gCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaaga < 1:1282255/71‑1 (MQ=255) gCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaaga < 1:1312685/71‑1 (MQ=255) gCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaaga < 1:3958847/71‑1 (MQ=255) tGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTATGTGAGAAGATGGGc > 1:1904629/1‑71 (MQ=255) tGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGc > 1:1003374/1‑71 (MQ=255) tGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGc > 1:2661979/1‑71 (MQ=255) tGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGc > 1:1324848/1‑71 (MQ=255) gCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTg > 1:1158519/1‑71 (MQ=255) cTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTg > 1:3628369/1‑52 (MQ=255) cTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTTAGAAGATGGGCGAGtt > 1:1909503/1‑68 (MQ=255) tgcgtgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAg < 1:435707/65‑1 (MQ=255) tgcgtgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAg < 1:110842/65‑1 (MQ=255) tgcgtgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAg < 1:922108/65‑1 (MQ=255) tgcgtgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAg < 1:2537169/65‑1 (MQ=255) cgtgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCg < 1:3715013/70‑1 (MQ=255) cgtgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAATTTGcc < 1:3109641/69‑1 (MQ=255) cgcAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTg > 1:739541/1‑71 (MQ=255) cgcAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTg > 1:816812/1‑71 (MQ=255) cgcAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTg > 1:4054400/1‑71 (MQ=255) cgcAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTg > 1:1735579/1‑71 (MQ=255) aGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTgcgc < 1:939419/71‑1 (MQ=255) aGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTgcgc < 1:3735729/71‑1 (MQ=255) aGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTgcgc < 1:3045995/71‑1 (MQ=255) aGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTgcgc < 1:428666/71‑1 (MQ=255) gTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTgcgc > 1:2964426/1‑70 (MQ=255) ttAGAAACTTTAGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAg < 1:2516490/63‑1 (MQ=255) ttAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAg < 1:889232/63‑1 (MQ=255) ttAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAg < 1:3177042/63‑1 (MQ=255) ttAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaa < 1:1344940/44‑1 (MQ=255) ttAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaa < 1:1927832/44‑1 (MQ=255) ttAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaa < 1:2837715/44‑1 (MQ=255) tAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGagaaga > 1:2391891/1‑45 (MQ=255) tAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGagaaga > 1:2232676/1‑45 (MQ=255) tAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGAt > 1:2875851/1‑71 (MQ=255) tAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGAt > 1:1516391/1‑71 (MQ=255) tAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGAGCGAt > 1:1065021/1‑71 (MQ=255) aGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCt > 1:2534333/1‑63 (MQ=255) aGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCt > 1:46724/1‑63 (MQ=255) aGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCt > 1:1408354/1‑63 (MQ=255) aGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCt > 1:4043517/1‑63 (MQ=255) aGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCt > 1:3789118/1‑63 (MQ=255) aGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCt > 1:3499672/1‑63 (MQ=255) aGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATc > 1:190557/1‑71 (MQ=255) aGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATc > 1:3053458/1‑71 (MQ=255) gAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATct > 1:3177558/1‑71 (MQ=255) aTAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGcgcg < 1:1240361/57‑1 (MQ=255) gTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATc > 1:2309640/1‑57 (MQ=255) gTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATc > 1:869544/1‑57 (MQ=255) gTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCa < 1:2539026/71‑1 (MQ=255) cGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcatc < 1:1983141/71‑1 (MQ=255) cGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcatc < 1:1816234/71‑1 (MQ=255) ggTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCg < 1:647482/71‑1 (MQ=255) tACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCa < 1:2078130/62‑1 (MQ=255) tACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCa < 1:865607/62‑1 (MQ=255) tACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCa < 1:2372903/62‑1 (MQ=255) tACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCa < 1:2160766/62‑1 (MQ=255) cAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGacac < 1:1023125/71‑1 (MQ=255) cAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGaca > 1:1039389/1‑70 (MQ=255) | TCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACAC > minE/1827872‑1827998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |