| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,874,329 | A→G | 37.7% | I191T (ATT→ACT) | ygcF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,874,329 | 0 | A | G | 37.7% | 116.8 / 154.9 | 138 | I191T (ATT→ACT) | ygcF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (46/40); new base G (42/10); total (88/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.73e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
Only 100 of 145 total aligned reads displayed.
TCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTCAAT > minE/1874261‑1874399 | tCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATc < 1:2863180/71‑1 (MQ=255)tCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATc < 1:1078344/71‑1 (MQ=255)tCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATc < 1:3739725/71‑1 (MQ=255) aaaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTg < 1:1162379/71‑1 (MQ=255) aaaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTg < 1:2934555/71‑1 (MQ=255) aaaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTg < 1:1375866/71‑1 (MQ=255) aaaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTg < 1:3622794/71‑1 (MQ=255) aaaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTg < 1:1395439/71‑1 (MQ=255) aaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTg > 1:2819419/1‑70 (MQ=255) aaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGc < 1:694954/71‑1 (MQ=255) gCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCATCCTTTTGGCTAGTCGGCTg > 1:2141454/1‑70 (MQ=255) gCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCATCCTTTTGGCTAGTCGGCTg > 1:1875691/1‑70 (MQ=255) gCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCATCCTTTTGGCTAGTCGGCTg > 1:3108766/1‑70 (MQ=255) gCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCATCCTTTTGGCTAGTCGGCTg > 1:157799/1‑70 (MQ=255) ccAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAAt > 1:3472700/1‑60 (MQ=255) aaTTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCa < 1:2263923/71‑1 (MQ=255) tACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAAt > 1:2139884/1‑70 (MQ=255) aCGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGa > 1:2537641/1‑71 (MQ=255) aCGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGa > 1:1618336/1‑71 (MQ=255) aCGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGa > 1:4078306/1‑71 (MQ=255) aCGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGa > 1:445991/1‑71 (MQ=255) cAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCaa > 1:6143/1‑58 (MQ=255) cAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCaa > 1:1834886/1‑58 (MQ=255) cAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCaa > 1:646792/1‑58 (MQ=255) cAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAAt > 1:949971/1‑59 (MQ=255) cAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAAt > 1:2030212/1‑59 (MQ=255) cAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAAt > 1:2420248/1‑59 (MQ=255) cAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAAt > 1:3081454/1‑59 (MQ=255) cAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAAt > 1:3370645/1‑59 (MQ=255) cAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAAt > 1:3441634/1‑59 (MQ=255) cAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAAt > 1:3451225/1‑59 (MQ=255) cAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAAt > 1:3912141/1‑59 (MQ=255) cAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGttt < 1:3526273/71‑1 (MQ=255) ggTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAAt < 1:2879911/57‑1 (MQ=255) ggTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAAt < 1:294484/57‑1 (MQ=255) ggTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAAt < 1:2640107/57‑1 (MQ=255) ttCAATGCACAAACGTGTTG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatcat > 1:2950983/1‑71 (MQ=255) ttCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTatcat > 1:2507915/1‑71 (MQ=255) ttCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGa > 1:1833388/1‑56 (MQ=255) ttCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGa > 1:2377141/1‑56 (MQ=255) ttCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatcat > 1:3193551/1‑71 (MQ=255) ttCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatca > 1:2474192/1‑70 (MQ=255) aaTGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatca < 1:2483081/67‑1 (MQ=255) aaTGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatca < 1:3074936/67‑1 (MQ=255) aaTGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatca < 1:729860/67‑1 (MQ=255) aaTGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatca < 1:2350552/67‑1 (MQ=255) aaTGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatca < 1:813945/67‑1 (MQ=255) cacaAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCa > 1:1777299/1‑40 (MQ=255) cacaAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCa > 1:3113715/1‑40 (MQ=255) cacaAACGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCTGTTTATCATCGGTCAg < 1:2401686/71‑1 (MQ=255) cacaAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAAt < 1:3863528/47‑1 (MQ=255) acaAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGc < 1:1037731/71‑1 (MQ=255) acaAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGc < 1:1960282/71‑1 (MQ=255) caAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCa > 1:525336/1‑38 (MQ=255) caAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCa > 1:2867507/1‑38 (MQ=255) caAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCa > 1:1993283/1‑38 (MQ=255) caAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCa > 1:1468981/1‑38 (MQ=255) caAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTat > 1:1082060/1‑59 (MQ=255) caAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTat > 1:1231934/1‑59 (MQ=255) aaCGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGtt < 1:3939371/71‑1 (MQ=255) aaCGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGtt < 1:3181069/71‑1 (MQ=255) aaCGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCa > 1:3864948/1‑36 (MQ=255) aaCGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCa > 1:407252/1‑36 (MQ=255) cGTGTGG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGc < 1:16920/71‑1 (MQ=255) cGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGt > 1:2089670/1‑68 (MQ=255) cGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGc < 1:558418/71‑1 (MQ=255) cGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGc < 1:4119650/71‑1 (MQ=255) gtgG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAg < 1:846977/62‑1 (MQ=255) tgG‑‑‑CATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCag > 1:3747608/1‑70 (MQ=255) tgG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCag < 1:2824779/70‑1 (MQ=255) tgG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCag < 1:3493418/70‑1 (MQ=255) tgG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCag < 1:2016858/70‑1 (MQ=255) gg‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCg > 1:3376827/1‑62 (MQ=255) atcCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTat > 1:3980609/1‑44 (MQ=255) atcCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTa > 1:1633822/1‑43 (MQ=255) atcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCa > 1:1446626/1‑70 (MQ=255) atcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCAt > 1:346909/1‑71 (MQ=255) atcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCAt > 1:4018028/1‑71 (MQ=255) cTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTc > 1:1205675/1‑49 (MQ=255) cTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTc > 1:93487/1‑49 (MQ=255) cTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTc > 1:1018840/1‑49 (MQ=255) cttttGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTc > 1:3563708/2‑48 (MQ=255) tttGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatca > 1:2622941/1‑41 (MQ=255) tttGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCgg > 1:1114879/1‑45 (MQ=255) tGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGc > 1:2998286/1‑71 (MQ=255) tGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGc > 1:1916663/1‑71 (MQ=255) tGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGc > 1:2220645/1‑71 (MQ=255) tGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTg < 1:183062/70‑1 (MQ=255) tGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTg < 1:2442516/70‑1 (MQ=255) cTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTc < 1:1404954/71‑1 (MQ=255) cTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTc < 1:2501599/71‑1 (MQ=255) cTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTc < 1:2446164/71‑1 (MQ=255) tAATCGGCTGCAGTGCAATGACTCGCGGTTTATCTTCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTc > 1:3623557/1‑70 (MQ=255) tAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTc > 1:1384408/1‑70 (MQ=255) tAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTc > 1:3883144/1‑70 (MQ=255) aTCGTCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTCAat < 1:3149356/71‑1 (MQ=255) aTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTTCTTCaa < 1:882217/70‑1 (MQ=255) aTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCttaat < 1:1051201/70‑4 (MQ=255) aTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTCaa < 1:3952820/70‑1 (MQ=255) aTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTCAat < 1:3829605/71‑1 (MQ=255) | TCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGG‑‑‑CATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTCAAT > minE/1874261‑1874399 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |