| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 204,556 | A→G | 100% | intergenic (+156/‑28) | alaV → / → rrlH | tRNA‑Ala/23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 204,556 | 0 | A | G | 100.0% | 232.9 / NA | 75 | intergenic (+156/‑28) | alaV/rrlH | tRNA‑Ala/23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/46); total (29/46) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCT > minE/204495‑204617 | ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:2075675/71‑1 (MQ=255) gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa < 1:3000638/70‑1 (MQ=255) gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa < 1:3784636/70‑1 (MQ=255) aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa < 1:2171343/69‑1 (MQ=255) aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa < 1:445942/69‑1 (MQ=255) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg < 1:177639/71‑1 (MQ=255) acaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg < 1:410055/70‑1 (MQ=255) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:4074394/1‑70 (MQ=25) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:431604/1‑70 (MQ=25) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:1375491/1‑70 (MQ=25) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:400858/1‑70 (MQ=25) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:1699783/1‑69 (MQ=25) aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:3268402/1‑69 (MQ=25) aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:1229770/1‑69 (MQ=25) aGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAgg > 1:4104283/1‑71 (MQ=21) tgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt > 1:2596536/1‑70 (MQ=25) ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg < 1:3957078/56‑1 (MQ=255) ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg < 1:2526767/56‑1 (MQ=255) ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg < 1:2508449/56‑1 (MQ=255) ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg < 1:1669763/56‑1 (MQ=255) ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg < 1:1576523/56‑1 (MQ=255) ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg < 1:1443515/56‑1 (MQ=255) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt < 1:1312947/60‑1 (MQ=255) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:713903/70‑1 (MQ=25) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:2908655/70‑1 (MQ=25) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:2599767/70‑1 (MQ=25) gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCg < 1:3590176/70‑1 (MQ=25) gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCg < 1:517677/70‑1 (MQ=25) gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCg < 1:828786/70‑1 (MQ=25) gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCg < 1:2513306/70‑1 (MQ=25) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAAc < 1:18708/41‑1 (MQ=255) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAAc < 1:3368839/41‑1 (MQ=255) ctctcAAATTTTCGCAACACGATGATGGATCGCAAGAATCa < 1:137852/41‑1 (MQ=14) ctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACa < 1:2901982/41‑1 (MQ=255) ctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACa < 1:1415012/41‑1 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg > 1:2720272/1‑71 (MQ=21) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg > 1:2698085/1‑71 (MQ=21) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg > 1:97322/1‑71 (MQ=21) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg > 1:2645504/1‑71 (MQ=21) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAACGACTAAGCg > 1:203357/1‑71 (MQ=255) tctcAAATTTTCGCAACACGATGAAGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg > 1:3906312/1‑71 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:2985229/69‑1 (MQ=25) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCtt > 1:581258/1‑40 (MQ=255) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCtt > 1:2810906/1‑40 (MQ=255) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:2342064/1‑71 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:3474711/1‑71 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:3800156/1‑71 (MQ=21) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTac < 1:3036037/70‑1 (MQ=25) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTac < 1:2683564/70‑1 (MQ=25) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa < 1:1325134/69‑1 (MQ=25) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa < 1:773982/69‑1 (MQ=25) aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac < 1:107229/71‑1 (MQ=21) ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGa > 1:1088154/1‑57 (MQ=255) ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGa > 1:1029458/1‑57 (MQ=255) ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGa > 1:1246223/1‑57 (MQ=255) tttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt > 1:1486506/1‑71 (MQ=21) tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:2473855/70‑1 (MQ=25) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc < 1:3541432/71‑1 (MQ=21) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc < 1:2745956/71‑1 (MQ=21) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc < 1:576827/71‑1 (MQ=21) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc < 1:480594/71‑1 (MQ=21) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc < 1:2423484/71‑1 (MQ=21) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc < 1:1750548/71‑1 (MQ=21) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc < 1:2148102/71‑1 (MQ=21) acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGcc > 1:1329280/1‑71 (MQ=21) acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGcc < 1:562796/71‑1 (MQ=21) acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGcc < 1:3870420/71‑1 (MQ=21) acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGcc < 1:2773798/71‑1 (MQ=21) acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGcc < 1:1057257/71‑1 (MQ=21) acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt < 1:19494/71‑1 (MQ=21) acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt < 1:501290/71‑1 (MQ=21) gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg > 1:4099078/1‑59 (MQ=255) gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg > 1:3537010/1‑59 (MQ=255) gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg > 1:2804565/1‑59 (MQ=255) gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg > 1:240324/1‑58 (MQ=255) gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAg > 1:1926080/1‑39 (MQ=255) gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGa > 1:490384/1‑42 (MQ=255) gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGa > 1:1056704/1‑42 (MQ=255) | TTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCT > minE/204495‑204617 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |