| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,883,198 | A→G | 26.4% | F429L (TTC→CTC) | rumA ← | 23S rRNA (uracil‑5)‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,883,198 | 0 | A | G | 26.4% | 187.4 / 79.7 | 110 | F429L (TTC→CTC) | rumA | 23S rRNA (uracil‑5)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (36/45); new base G (24/5); total (60/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.20e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
Only 100 of 138 total aligned reads displayed.
AACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATC > minE/1883128‑1883269 | aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:172995/71‑1 (MQ=255)aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:1913461/71‑1 (MQ=255)aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:2844726/71‑1 (MQ=255) aCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaaa < 1:1763435/71‑1 (MQ=255) aCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaaa < 1:3327997/71‑1 (MQ=255) aCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa > 1:2698469/1‑70 (MQ=255) aTCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAg > 1:3552685/1‑70 (MQ=255) tCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGt < 1:2965868/70‑1 (MQ=255) gTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTa > 1:2977156/1‑69 (MQ=255) gTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTAc > 1:3080079/1‑70 (MQ=255) gTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTAc < 1:2831944/70‑1 (MQ=255) gTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTAc > 1:1148648/1‑70 (MQ=255) gTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTAc > 1:3569135/1‑70 (MQ=255) ctctCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATc < 1:3601750/71‑1 (MQ=255) ctctCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATc < 1:3616077/71‑1 (MQ=255) tctcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:2092425/60‑1 (MQ=255) ctcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaaaa > 1:1004336/1‑61 (MQ=255) ctcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaaaa > 1:2420987/1‑61 (MQ=255) ctcCTATAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaaaa > 1:765367/1‑61 (MQ=255) tcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATc < 1:3147319/68‑1 (MQ=255) tcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGAt > 1:54833/1‑71 (MQ=255) ccTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGt > 1:483494/1‑61 (MQ=255) ccTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGAtt > 1:3583269/1‑71 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAg > 1:3713502/1‑59 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAg > 1:2721518/1‑59 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAg > 1:2443891/1‑59 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAg > 1:422402/1‑59 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTc > 1:2759620/1‑71 (MQ=255) tttAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:2022116/55‑1 (MQ=255) tAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCa < 1:2573488/70‑1 (MQ=255) cAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTccc < 1:1166397/71‑1 (MQ=255) cAGACCTGACGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTccc < 1:1408172/71‑1 (MQ=255) cTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTccc > 1:418916/1‑66 (MQ=255) cTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTccc > 1:3840882/1‑66 (MQ=255) gCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGtgtgtg > 1:1061738/1‑71 (MQ=255) gCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTAcct > 1:826961/1‑46 (MQ=255) gCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCAt > 1:2317905/1‑48 (MQ=255) gCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCAt > 1:1720380/1‑48 (MQ=255) gCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCAt > 1:2313045/1‑48 (MQ=255) gCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCAt > 1:42771/1‑48 (MQ=255) ccGAAATCTGCAAATCGCAACTATTTAACGCGCGAGAAAAGTAcc < 1:4107075/45‑1 (MQ=255) ccGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTAcc < 1:2248518/45‑1 (MQ=255) ccGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTgg > 1:75959/1‑71 (MQ=255) ccGAAATCGGCAAATCGCAACTATTTAAAGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTgg > 1:3936513/1‑71 (MQ=255) aaaTCGGCAAATCTCAACTATTTAACGCGCGAGaaa < 1:171436/36‑1 (MQ=255) aaaTCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:1268460/35‑1 (MQ=255) aaaTCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:2749984/35‑1 (MQ=255) aTCGGCAAATCGCAACTATTTAACGCGCGAGaaaa > 1:1476626/1‑35 (MQ=255) aTCGGCAAATCGCAACTATTTAACGCGCGAGaaaa > 1:1475390/1‑35 (MQ=255) ggCAGATCGCAACTATTTAACGCGCGAGAGAAGTAc > 1:555120/1‑36 (MQ=37) ggCAAATCGCAACTATTTAACGCGCGAGAGAAGTAc > 1:679391/1‑36 (MQ=255) cAAATCGCAACTATTTAACGCGGGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa > 1:3143299/1‑64 (MQ=255) cAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGa < 1:636552/40‑1 (MQ=255) cAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGa < 1:1584545/40‑1 (MQ=255) cAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGa < 1:2165741/40‑1 (MQ=255) cAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGa < 1:3328761/40‑1 (MQ=255) cAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa > 1:1517817/1‑64 (MQ=255) cAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa > 1:304719/1‑64 (MQ=255) cAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa > 1:2020223/1‑64 (MQ=255) aaTCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg < 1:2072864/54‑1 (MQ=255) aaTCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg < 1:3446343/54‑1 (MQ=255) aaTCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg < 1:1336229/54‑1 (MQ=255) aaTCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAg < 1:3801319/71‑1 (MQ=255) aTCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTcc > 1:1945203/1‑49 (MQ=255) tCGCATCTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCa < 1:2955511/71‑1 (MQ=255) tCGCAACTATTTAACGCGCGATAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCa < 1:2761401/71‑1 (MQ=255) tCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACat < 1:3081834/63‑1 (MQ=255) tCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCa < 1:1251609/71‑1 (MQ=255) tCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCa < 1:1789939/71‑1 (MQ=255) tCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCa < 1:1502728/71‑1 (MQ=255) aCTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGa < 1:2082277/54‑1 (MQ=255) cTATTTAACGCGCGAGAAAAGTACCATCGATTCCAg < 1:3236106/36‑1 (MQ=255) cTATTTAACGCGCGAGAAAAGTACCATCGATTCCAg < 1:1922720/36‑1 (MQ=255) cTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGc > 1:953991/1‑69 (MQ=255) aTTTAACGCGCGAGAAAAGTACCATCGATTCCTGAt > 1:257435/1‑36 (MQ=255) aTTTAACGCGCGAGAAAAGTACCATCGATTCCAGAt > 1:3548010/1‑36 (MQ=255) aTTTAACGCGCGAGAAAAGTACCATCGATTCCAGAt > 1:342067/1‑36 (MQ=255) tttAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt < 1:3201247/70‑1 (MQ=255) ttAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCa > 1:1300179/1‑58 (MQ=255) ttAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCa > 1:1318970/1‑58 (MQ=255) tAACGCGCGAGAGAAGTACCATCGATTCCAGATGt > 1:349423/1‑35 (MQ=255) tAACGCGCGAGAGAAGTACCATCGATTCCAGATGt > 1:718859/1‑35 (MQ=255) tAACGCGCGAGAGAAGTACCATCGATTCCAGATGt > 1:3974881/1‑35 (MQ=255) tAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgc > 1:3685684/1‑71 (MQ=255) aCGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCgcgc < 1:1425152/71‑1 (MQ=255) cgAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATgg < 1:1187363/71‑1 (MQ=255) gagagaAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGt < 1:416098/71‑1 (MQ=255) gagagaAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGt < 1:3318030/71‑1 (MQ=255) gagagaAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGt < 1:2162145/71‑1 (MQ=255) gagagaAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGt < 1:655732/71‑1 (MQ=255) gagaAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATc > 1:2373090/1‑47 (MQ=255) gagaAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATc > 1:600146/1‑47 (MQ=255) gagaAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATc > 1:3801298/1‑47 (MQ=255) gagaAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGt < 1:3400839/71‑1 (MQ=255) gagaAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGt < 1:922314/71‑1 (MQ=255) agaAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGta < 1:3161308/71‑1 (MQ=255) agaAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGta < 1:1388778/71‑1 (MQ=255) gaAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGtat < 1:2688164/71‑1 (MQ=255) aaaaGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGtata > 1:1033843/1‑71 (MQ=255) aaaaGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGT‑‑CGCAATGGTATATc > 1:3509586/1‑71 (MQ=255) | AACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATC > minE/1883128‑1883269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |