| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,914,848 | T→C | 27.0% | E208E (GAA→GAG) | ptsP ← | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,914,848 | 0 | T | C | 27.0% | 55.7 / 24.8 | 37 | E208E (GAA→GAG) | ptsP | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Reads supporting (aligned to +/- strand): ref base T (16/11); new base C (8/2); total (24/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01 | |||||||||||
GCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGC > minE/1914785‑1914914 | gCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCaga < 1:3906181/70‑1 (MQ=255) gTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGAt > 1:727762/1‑68 (MQ=255) gcggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGc > 1:1444942/1‑70 (MQ=255) gcggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAGc < 1:1967345/70‑1 (MQ=255) ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCTAGCGtt < 1:6364/71‑1 (MQ=255) ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGtt < 1:1179133/71‑1 (MQ=255) ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGtt < 1:2743590/71‑1 (MQ=255) ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGtt < 1:2549809/71‑1 (MQ=255) ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGtt < 1:2533461/71‑1 (MQ=255) gcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGtt < 1:3251780/70‑1 (MQ=255) gAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGAt > 1:1399678/1‑71 (MQ=255) gAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGAt > 1:3025403/1‑71 (MQ=255) cTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAg < 1:901866/60‑1 (MQ=255) cTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAg < 1:355716/60‑1 (MQ=255) cTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAg < 1:225256/60‑1 (MQ=255) cTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAg < 1:406677/60‑1 (MQ=255) cTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCt > 1:2199009/1‑71 (MQ=255) gTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGcc > 1:2737540/1‑49 (MQ=255) gTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGcc > 1:811077/1‑49 (MQ=255) tGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAGCGTTGa > 1:2913807/1‑60 (MQ=255) ccAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCAt > 1:3736465/1‑70 (MQ=255) ccccGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAgg > 1:3132152/1‑70 (MQ=255) ccccGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAgg > 1:3478122/1‑70 (MQ=255) ccccGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATGAAAgg > 1:3398566/1‑70 (MQ=255) ccGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTaa > 1:2298805/1‑71 (MQ=255) ccGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTaa < 1:483276/71‑1 (MQ=255) gTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATcc > 1:982738/1‑71 (MQ=255) gTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATcc > 1:3278135/1‑71 (MQ=255) gTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATcc > 1:2074025/1‑71 (MQ=255) gTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATcc > 1:1772263/1‑71 (MQ=255) gTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATcc > 1:1605045/1‑71 (MQ=255) gTTCGCGCTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATcc > 1:3113956/1‑71 (MQ=255) cgcgTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTg > 1:2273967/1‑70 (MQ=255) cgcgTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTg > 1:1370299/1‑70 (MQ=255) cgcgTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTg > 1:1153198/1‑70 (MQ=255) cgcgTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGc > 1:4096821/1‑71 (MQ=255) cgcgTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGc > 1:1060233/1‑71 (MQ=255) ttCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGc < 1:3997325/60‑1 (MQ=255) ttCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGc < 1:1347089/60‑1 (MQ=255) | GCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGC > minE/1914785‑1914914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |