BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,993,572	G→A	35.0%	V289M (GTG→ATG)	nupG →	nucleoside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,993,572	0	G	A	35.0%	188.7 / 160.8	160	V289M (GTG→ATG)	nupG	nucleoside transporter
Reads supporting (aligned to +/- strand): ref base G (55/49); new base A (40/16); total (95/65)
Fisher's exact test for biased strand distribution p-value = 2.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

TTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGG  >  minE/1993506‑1993641

ttCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATtatg                                                                     <  1:1848412/69‑1 (MQ=255)

ttCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATtatg                                                                     <  1:2476797/69‑1 (MQ=255)

ttCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATtatg                                                                     <  1:2611048/69‑1 (MQ=255)

ttCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATtatg                                                                     <  1:2650874/69‑1 (MQ=255)

  cATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCg                                                                  <  1:3982565/70‑1 (MQ=255)

  cATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCg                                                                  <  1:1686136/70‑1 (MQ=255)

  cATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCg                                                                  <  1:2723723/70‑1 (MQ=255)

  cATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCg                                                                  <  1:368755/70‑1 (MQ=255)

  cATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGt                                                                 >  1:3760730/1‑71 (MQ=255)

     tCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGa                                                              >  1:2450258/1‑71 (MQ=255)

     tCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGa                                                              >  1:3077038/1‑71 (MQ=255)

     tCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGa                                                              >  1:1566326/1‑71 (MQ=255)

      cTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATtg                                                                       >  1:2953561/1‑61 (MQ=255)

      cTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATtg                                                                       >  1:3088246/1‑61 (MQ=255)

      cTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGAt                                                             >  1:2754835/1‑71 (MQ=255)

      atGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATtg                                                                       >  1:1450042/2‑61 (MQ=255)

          ccATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTg                                                         <  1:3280171/71‑1 (MQ=255)

           cATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGc                                                        >  1:1666509/1‑71 (MQ=255)

               ccGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCg                                                                  >  1:1000755/1‑57 (MQ=255)

               ccGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGttt                                                    >  1:553407/1‑71 (MQ=255)

               ccGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGttt                                                    >  1:2444164/1‑71 (MQ=255)

               ccGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGttt                                                    >  1:4021352/1‑71 (MQ=255)

               ccGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGttt                                                    >  1:211058/1‑71 (MQ=255)

               ccGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGttt                                                    >  1:103448/1‑71 (MQ=255)

                 gTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTg                                                  <  1:823852/71‑1 (MQ=255)

                  ttcttcTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTgc                                                 <  1:3605364/71‑1 (MQ=255)

                  ttcttcTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTCGATCCTGCGTTTTgc                                                 <  1:1276040/71‑1 (MQ=255)

                    cttcttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTgcgc                                               >  1:1693855/1‑71 (MQ=255)

                    cttcttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTgcgc                                               >  1:3095485/1‑71 (MQ=255)

                    cttcttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTgcgc                                               >  1:3713508/1‑71 (MQ=255)

                    cttcttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTgcg                                                <  1:1386263/70‑1 (MQ=255)

                    cttcttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTgcg                                                <  1:447076/70‑1 (MQ=255)

                    cttcttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTgcgc                                               >  1:2688829/1‑71 (MQ=255)

                       cttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGt                                            >  1:4094222/1‑71 (MQ=255)

                       cttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGt                                            >  1:2736665/1‑71 (MQ=255)

                         tAATCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGt                                                                 >  1:2256699/1‑48 (MQ=39)

                         tAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATc                                                            >  1:2308481/1‑53 (MQ=255)

                         tAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATc                                                            >  1:3344357/1‑53 (MQ=255)

                         tAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATc                                                            >  1:1192999/1‑53 (MQ=255)

                         tAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATc                                                            >  1:2990375/1‑53 (MQ=255)

                         tAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATc                                                            >  1:592644/1‑53 (MQ=255)

                          aaGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGt                                            <  1:3966404/68‑1 (MQ=255)

                           aGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTg                                         <  1:3930253/70‑1 (MQ=255)

                           aGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTg                                         <  1:3641475/70‑1 (MQ=255)

                            gCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCt                                       <  1:1226747/71‑1 (MQ=255)

                               gCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGGTTAc                                    <  1:4125196/71‑1 (MQ=255)

                               gCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTAc                                    <  1:2245481/71‑1 (MQ=255)

                               gCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTAc                                    <  1:424592/71‑1 (MQ=255)

                                 tACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACgg                                  >  1:2590504/1‑71 (MQ=255)

                                 tACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACgg                                  >  1:1410799/1‑71 (MQ=255)

                                 tACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACgg                                  >  1:677621/1‑71 (MQ=255)

                                  aCGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGc                                 >  1:1916297/1‑71 (MQ=255)

                                  aCGGTATTAAGAACGTAATGATGATCAGTATTGTGGAGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGc                                 >  1:2167198/1‑71 (MQ=255)

                                   cGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGc                                 <  1:2755526/70‑1 (MQ=255)

                                        ttAAGAACGTAATGATGATCAGTATTGTGGCGTGGa                                                              <  1:235712/36‑1 (MQ=255)

                                        ttAAGAACGTAATGATGATCAGTATTGTGGCGTGGa                                                              <  1:1627218/36‑1 (MQ=255)

                                        ttAAGAACGTAATGATGATCAGTATTGTGGCGTGGa                                                              <  1:2215328/36‑1 (MQ=255)

                                        ttAAGAACGTAATGATGATCAGTATTGTGGCGTGGa                                                              <  1:3773146/36‑1 (MQ=255)

                                        ttAAGAACGTAATGATGATCAGTATTGTGGCGTGGATcc                                                           >  1:1810441/1‑39 (MQ=255)

                                          aaGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGttt                                          >  1:4025877/1‑54 (MQ=255)

                                          aaGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGttt                                          >  1:3162491/1‑54 (MQ=255)

                                          aaGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGttt                                          >  1:572740/1‑54 (MQ=255)

                                          aaGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTg                                         >  1:4103405/1‑55 (MQ=255)

                                          aaGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTg                                         >  1:3581626/1‑55 (MQ=255)

                                          aaGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGtt                                           >  1:1592786/1‑53 (MQ=255)

                                          aaGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGtt                                           >  1:601962/1‑53 (MQ=255)

                                          aaGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGtt                                           >  1:2283433/1‑53 (MQ=255)

                                          aaGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGtt                                           >  1:2539903/1‑53 (MQ=255)

                                           aGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACt                        >  1:3511083/1‑71 (MQ=255)

                                           aGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACt                        >  1:1148000/1‑71 (MQ=255)

                                           aGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACt                        >  1:3187145/1‑71 (MQ=255)

                                           aGAACGAAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACt                        >  1:533951/1‑71 (MQ=255)

                                            gAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTg                                             <  1:3282726/49‑1 (MQ=255)

                                            gAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTg                                             <  1:2482858/49‑1 (MQ=255)

                                                   atgatgATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTa                                     >  1:512813/1‑50 (MQ=255)

                                                   atgatgATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCgg                <  1:1477441/71‑1 (MQ=255)

                                                   atgatgATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCgg                <  1:3861154/71‑1 (MQ=255)

                                                   atgatgATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCgg                <  1:1162522/71‑1 (MQ=255)

                                                   atgatgATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCgg                <  1:2952045/71‑1 (MQ=255)

                                                   atgatgATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCgg                <  1:2905402/71‑1 (MQ=255)

                                                    tgatgaTCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGAccc                            <  1:815829/58‑1 (MQ=255)

                                                      atgatCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCg                 >  1:2394963/1‑67 (MQ=255)

                                                      atgatCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCg                 >  1:1319676/1‑67 (MQ=255)

                                                      atgatCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCg                 >  1:3066175/1‑67 (MQ=255)

                                                      atgatCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCg                 >  1:1091630/1‑67 (MQ=255)

                                                         atCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGgtactg           <  1:902457/70‑1 (MQ=255)

                                                         atCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGgtactg           <  1:73107/70‑1 (MQ=255)

                                                           cAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTa                                     >  1:2769100/1‑42 (MQ=255)

                                                           cAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTa                                     >  1:2089354/1‑42 (MQ=255)

                                                           cAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTa                                     >  1:861505/1‑42 (MQ=255)

                                                           cAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTa                                     >  1:3718184/1‑42 (MQ=255)

                                                            aGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGAccc                            <  1:100532/50‑1 (MQ=255)

                                                            aGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGAccc                            <  1:2525230/50‑1 (MQ=255)

                                                            aGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGAccc                            <  1:1323005/50‑1 (MQ=255)

                                                            aGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTc                  >  1:1739480/1‑60 (MQ=255)

                                                               aTTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTActgct    >  1:80934/1‑71 (MQ=255)

                                                                  gtgGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTa                                     >  1:2374380/1‑35 (MQ=255)

                                                                  gtgGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTa                                     >  1:2126173/1‑35 (MQ=255)

                                                                  gtgGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTgg  >  1:2058306/1‑70 (MQ=255)

                                                                  gtgGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTgg  >  1:984842/1‑70 (MQ=255)

TTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGG  >  minE/1993506‑1993641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`