| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,004,302 | G→A | 25.5% | S483F (TCT→TTT) | hybC ← | hydrogenase 2, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,004,302 | 0 | G | A | 25.5% | 89.9 / 32.3 | 51 | S483F (TCT→TTT) | hybC | hydrogenase 2, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (23/15); new base A (7/6); total (30/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
TTCCAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCT > minE/2004236‑2004371 | ttCCAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGagagag < 1:2792347/71‑1 (MQ=255)ttCCAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGagagag < 1:526805/71‑1 (MQ=255)ttCCAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGagagag < 1:4096393/71‑1 (MQ=255)ttCCAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGagagag < 1:1886707/71‑1 (MQ=255)ttCCAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGagagag < 1:1806099/71‑1 (MQ=255)ttCCAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGagagag < 1:3351350/71‑1 (MQ=255) ggTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCa > 1:1173531/1‑68 (MQ=255) gAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCgcggc > 1:796717/1‑71 (MQ=255) gAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCgcggc > 1:1975740/1‑71 (MQ=255) gAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCgcggc > 1:3004542/1‑71 (MQ=255) tGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGagagag < 1:1032762/47‑1 (MQ=255) tGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGagagag < 1:2182522/47‑1 (MQ=255) tGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGagagag < 1:21685/47‑1 (MQ=255) tGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGagagag < 1:244455/47‑1 (MQ=255) tGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGagagag < 1:2675002/47‑1 (MQ=255) tGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGagagag < 1:1607632/47‑1 (MQ=255) tGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGagagag < 1:1086657/47‑1 (MQ=255) gTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATAc < 1:3623195/47‑1 (MQ=255) gTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCaa < 1:1363944/71‑1 (MQ=255) tGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAaca > 1:768711/1‑71 (MQ=255) gCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGc > 1:1711227/1‑40 (MQ=255) gCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGc > 1:2677734/1‑40 (MQ=255) gCTGATGAAACCGTCTTTAATAACCATCCAGTGAGAGAGc > 1:2862085/1‑40 (MQ=255) tgatACCGTCTTTAATAACCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCttt > 1:1849045/1‑70 (MQ=255) tgatACAGTCTTTAATAACCATCCAGTGAAAGAGCATAC‑‑CGCGGCGCTTCGAGGAAGCCAACACCTTTGaa > 1:4091182/1‑71 (MQ=255) gatACCGTCTTTAATAACCATCCAGTGAGAGAGCAt > 1:2819245/1‑36 (MQ=255) gatACCGTCTTTAATAACCATCCAGTGAGAGAGCAt > 1:1983948/1‑36 (MQ=255) gatACCGTCTTTAATAACCATCCAGTGAGAGAGCAt > 1:716080/1‑36 (MQ=255) gatACCGTCTTTAATAACCATCCAGTGAGAGAGCAt > 1:2393396/1‑36 (MQ=255) ccGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTc < 1:767870/47‑1 (MQ=255) ccGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTc < 1:994658/47‑1 (MQ=255) ccGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTc < 1:1244379/47‑1 (MQ=255) ccGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAAtt < 1:239590/70‑1 (MQ=255) ccGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAAtt < 1:921205/70‑1 (MQ=255) ccGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAAtt < 1:2185598/70‑1 (MQ=255) tCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCttg < 1:3292371/62‑2 (MQ=255) tCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTg < 1:1056342/63‑1 (MQ=255) tCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTg < 1:287550/63‑1 (MQ=255) tCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTg < 1:2136103/63‑1 (MQ=255) tCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTg < 1:3209439/63‑1 (MQ=255) tCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTg < 1:1866150/63‑1 (MQ=255) tttAATAACCATCCAGTGAGAGAGAATACCGCGCGGCGCTTCGAGGaa > 1:2153782/1‑48 (MQ=255) taataaCCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCg > 1:2507516/1‑41 (MQ=255) taataaCCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCAccc > 1:1192246/1‑68 (MQ=255) taataaCCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGtt > 1:1574120/1‑71 (MQ=255) taataaCCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGtt > 1:1461672/1‑71 (MQ=255) taataaCCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGtt > 1:1793950/1‑71 (MQ=255) taataaCCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGtt > 1:295081/1‑71 (MQ=255) taataaCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGcc > 1:1244942/1‑49 (MQ=255) taataaCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGcc > 1:2115773/1‑49 (MQ=255) taataaCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGcc > 1:1074670/1‑49 (MQ=255) taataaCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCAccc < 1:2991761/68‑1 (MQ=255) taataaCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCAccc < 1:3868552/68‑1 (MQ=255) taataaCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCg > 1:85568/1‑69 (MQ=255) aataaCCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTg > 1:3588732/1‑71 (MQ=255) aataaCCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTg > 1:911319/1‑71 (MQ=255) aataaCCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTg > 1:3128860/1‑71 (MQ=255) aataaCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCaa > 1:1104451/1‑50 (MQ=255) aCCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCgg < 1:2918271/71‑1 (MQ=255) cATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGaa < 1:1560612/71‑1 (MQ=255) aTCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGaa < 1:2099719/70‑1 (MQ=255) aTCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGaa < 1:871335/70‑1 (MQ=255) cAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGaa > 1:2961884/1‑50 (MQ=255) tGAAAGAGCATACCGCGCTGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGaa < 1:1922583/64‑1 (MQ=255) gagagaGCATACCGCGCGGCGCTTCGAGGAAG‑CAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCt > 1:967084/1‑71 (MQ=255) agagagCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAAtt > 1:3792683/1‑47 (MQ=255) agagagCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCt > 1:1922444/1‑71 (MQ=255) agagagCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCt > 1:244306/1‑71 (MQ=255) | TTCCAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCT > minE/2004236‑2004371 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |