| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,266,165 | G→A | 8.5% | I93I (ATC→ATT) | tufB ← | protein chain elongation factor EF‑Tu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,266,165 | 0 | G | A | 8.5% | 243.1 / 11.0 | 82 | I93I (ATC→ATT) | tufB | protein chain elongation factor EF‑Tu |
| Reads supporting (aligned to +/- strand): ref base G (22/53); new base A (4/3); total (26/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
TGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACG > minE/2266100‑2266221 | tGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTg > 1:2444178/1‑66 (MQ=35) cGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCa < 1:303772/68‑1 (MQ=35) cGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGt > 1:2030171/1‑71 (MQ=34) aTCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGttttt < 1:2220942/71‑1 (MQ=34) aTCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGtttt > 1:847347/1‑70 (MQ=35) aTCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGtttt > 1:3676447/1‑70 (MQ=35) aTCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGtttt > 1:1681166/1‑70 (MQ=35) gCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACAt < 1:4080080/71‑1 (MQ=34) cGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAg > 1:2214776/1‑71 (MQ=34) aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg < 1:207808/71‑1 (MQ=34) aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg < 1:1708909/71‑1 (MQ=34) aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg < 1:2604164/71‑1 (MQ=34) aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg < 1:2896524/71‑1 (MQ=34) aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg < 1:3242487/71‑1 (MQ=34) aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg < 1:3456591/71‑1 (MQ=34) aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg < 1:1313566/71‑1 (MQ=34) aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg < 1:3811258/71‑1 (MQ=34) aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg < 1:488322/71‑1 (MQ=34) cGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGt > 1:2704317/1‑71 (MQ=34) cGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGt > 1:1171278/1‑71 (MQ=34) cagcaACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGc < 1:1959267/71‑1 (MQ=34) cagcaACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGc < 1:586808/71‑1 (MQ=34) agcaACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGc < 1:245214/70‑1 (MQ=35) cTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCggg < 1:1049692/71‑1 (MQ=34) cTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCg < 1:3857596/69‑1 (MQ=35) cTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCg < 1:960805/69‑1 (MQ=35) cTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCg < 1:315436/69‑1 (MQ=35) cTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCg < 1:774507/69‑1 (MQ=35) tACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCggg > 1:2245598/1‑70 (MQ=35) tACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCggg > 1:672111/1‑70 (MQ=35) tACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCggg > 1:774919/1‑70 (MQ=35) ccAGGATCGCGCCGTCCATCTGCGCAGCACCGTTAATCATGt < 1:824885/42‑1 (MQ=14) ccAGGATCGCGCCGTCCATCTGCGCAGCACCGGTAATCATGt < 1:3783183/42‑1 (MQ=255) ccAGGATCGCGCCGTCCATCTGCGCAGCACCGGTAATCATGt < 1:3282436/42‑1 (MQ=255) aGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAAGATAGTCGGCGTGCCCCGGGCAGTc < 1:2061377/71‑1 (MQ=255) aGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTc < 1:1794439/71‑1 (MQ=34) aGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTc < 1:3061830/71‑1 (MQ=34) aGGATCGCGCCGTCCATCTGCGCAGCACCGGTAATCAttttc > 1:3956215/1‑41 (MQ=14) aGGATCGCGCCGTCCATCTGCGCAGCACCGGTAATCATGttc > 1:1956574/1‑41 (MQ=255) aGGATCGCGCCGTCCATCTGCGCAGCACCGGTAATCATGttc > 1:1366812/1‑41 (MQ=255) aGGATCGCGCCGTCCATCTGCGCAGCACCGGTAATCATGttc > 1:3944035/1‑41 (MQ=255) aTCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc > 1:2895770/1‑71 (MQ=34) aTCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc < 1:3072901/71‑1 (MQ=34) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTa > 1:1237610/1‑69 (MQ=35) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACg < 1:86365/71‑1 (MQ=34) cgcgCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCg > 1:947503/1‑52 (MQ=35) cgcgCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACgt > 1:149366/1‑71 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:2313121/71‑1 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:1247417/71‑1 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:3567389/71‑1 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:1313798/71‑1 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:3916690/71‑1 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:3271261/71‑1 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:3211378/71‑1 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:498780/71‑1 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:2931751/71‑1 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:2819101/71‑1 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:79405/71‑1 (MQ=34) gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt < 1:995638/71‑1 (MQ=34) gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt < 1:81104/71‑1 (MQ=34) gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt < 1:2805033/71‑1 (MQ=34) gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt < 1:2209707/71‑1 (MQ=34) gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt < 1:1997590/71‑1 (MQ=34) gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt < 1:2426695/71‑1 (MQ=34) gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt < 1:182496/71‑1 (MQ=34) gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt < 1:1506427/71‑1 (MQ=34) gTCAATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt < 1:1001938/71‑1 (MQ=21) tCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt < 1:3548966/70‑1 (MQ=35) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:3104566/71‑1 (MQ=34) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:3025153/71‑1 (MQ=34) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:1368268/71‑1 (MQ=34) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:3825143/71‑1 (MQ=34) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:2729399/71‑1 (MQ=34) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:2629231/71‑1 (MQ=34) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:2466890/71‑1 (MQ=34) ttcgcAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACg > 1:127561/3‑71 (MQ=14) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc > 1:1537552/1‑56 (MQ=35) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc > 1:1258442/1‑56 (MQ=35) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc > 1:4122398/1‑56 (MQ=35) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACg > 1:939556/1‑57 (MQ=35) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACg > 1:1595045/1‑71 (MQ=34) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACg > 1:449318/1‑71 (MQ=34) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACg > 1:3680251/1‑71 (MQ=34) | TGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACG > minE/2266100‑2266221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |