| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,283,783 | G→T | 24.0% | L39M (CTG→ATG) | oxyR ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,283,783 | 0 | G | T | 24.0% | 154.8 / 22.8 | 58 | L39M (CTG→ATG) | oxyR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base G (26/18); new base T (2/12); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
TTCCCGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAATGGC > minE/2283714‑2283851 | ttCCCGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGc > 1:266468/1‑71 (MQ=255)ttCCCGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGc > 1:463161/1‑71 (MQ=255) cccGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAg > 1:3036929/1‑68 (MQ=255) cccGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCt < 1:636302/70‑1 (MQ=255) cccGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCt > 1:2374714/1‑70 (MQ=255) ccGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCtt < 1:2065336/70‑1 (MQ=255) gCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACg < 1:337629/71‑1 (MQ=255) gCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACg < 1:2801168/71‑1 (MQ=255) ccTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGa < 1:469118/71‑1 (MQ=255) ccTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGa < 1:3967384/71‑1 (MQ=255) cTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGaa < 1:546291/71‑1 (MQ=255) cTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGaa < 1:2623357/71‑1 (MQ=255) cTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGaa < 1:2472279/71‑1 (MQ=255) cTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGa < 1:2304735/70‑1 (MQ=255) cTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGa < 1:3485474/70‑1 (MQ=255) ggTGAACAACACTTTACGGCTTGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAAtt < 1:2637655/70‑1 (MQ=255) ggTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAAttt < 1:2944256/71‑1 (MQ=255) ggTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAAttt < 1:1566960/71‑1 (MQ=255) ggTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAAttt < 1:1452820/71‑1 (MQ=255) ggTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAAttt < 1:218303/71‑1 (MQ=255) aacaCTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCt < 1:632430/71‑1 (MQ=255) aacaCTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCt < 1:49346/71‑1 (MQ=255) acacTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTa < 1:3328813/71‑1 (MQ=255) cTTTACGGCTTGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGATAAGc < 1:2087250/71‑1 (MQ=255) cTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGATAAGc < 1:61223/71‑1 (MQ=255) cTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGATAAGc < 1:1725101/71‑1 (MQ=255) tACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAg > 1:610513/1‑67 (MQ=255) tACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAg > 1:1774771/1‑67 (MQ=255) gTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCggctggct < 1:2535909/71‑1 (MQ=255) ccGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTaa > 1:2182498/1‑71 (MQ=255) cTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGt < 1:2397176/71‑1 (MQ=255) cTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGt < 1:3849316/71‑1 (MQ=255) cTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGt < 1:74742/71‑1 (MQ=255) cAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCggctggct > 1:1837072/1‑63 (MQ=255) cAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTgg > 1:2934284/1‑70 (MQ=255) gCAACATCACGCCCAGCTCATCTTCCAGCTTACGAAt > 1:580894/1‑37 (MQ=255) gCAACATCACGCCCAGCTCATCTTCCAGCTTACGAAt > 1:2495383/1‑37 (MQ=255) gCAACATCACGCCCAGCTCATCTTCCAGCTTACGAAt > 1:3309404/1‑37 (MQ=255) gCAACATCACGCCCAGCTCATCTTCCAGCTTACGAAt > 1:2300349/1‑37 (MQ=255) gCAACATCACGCCCAGCTCATCTTCCAGCTTACGAAt > 1:1193937/1‑37 (MQ=255) aCATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGa > 1:56553/1‑70 (MQ=255) aTCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGt > 1:1973091/1‑61 (MQ=255) aTCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGan > 1:2464969/1‑68 (MQ=255) aTCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAAt > 1:3120234/1‑70 (MQ=255) aTCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAAt > 1:1698813/1‑70 (MQ=255) aTCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAAt > 1:151248/1‑70 (MQ=255) aCGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGc > 1:2383089/1‑71 (MQ=255) aCGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGc < 1:816479/71‑1 (MQ=255) gCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGt < 1:129745/51‑1 (MQ=255) cTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGaaaa > 1:2020715/1‑71 (MQ=255) ttCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAAt > 1:3975913/1‑71 (MQ=255) ttCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATc > 1:3722423/1‑54 (MQ=255) ttCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATc > 1:2692164/1‑54 (MQ=255) ttCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAAt > 1:440910/1‑71 (MQ=255) ttCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAAt > 1:2777804/1‑71 (MQ=255) ttCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAAt > 1:1714175/1‑71 (MQ=255) ccAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAATgg > 1:44950/1‑71 (MQ=255) cAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAATGGc < 1:2066098/71‑1 (MQ=255) cAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAATGGc < 1:676859/71‑1 (MQ=255) | TTCCCGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAATGGC > minE/2283714‑2283851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |