| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,321,698 | G→A | 22.8% | T93I (ACC→ATC) | cdh ← | CDP‑diacylglycerol phosphotidylhydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,321,698 | 0 | G | A | 22.8% | 236.0 / 67.2 | 114 | T93I (ACC→ATC) | cdh | CDP‑diacylglycerol phosphotidylhydrolase |
| Reads supporting (aligned to +/- strand): ref base G (39/49); new base A (18/8); total (57/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
GTATTTTTTGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCATT > minE/2321631‑2321765 | gTATTTTTTGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTc > 1:2417292/1‑70 (MQ=255)gTATTTTTTGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTc > 1:2240684/1‑70 (MQ=255) ttttttGCTCATAAAATCACGCGCCTGCCAAGCCACCCAAAAGAAGTTCGGCGTTGAAGGATCGGTcaac > 1:3323338/1‑70 (MQ=255) ttttttGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTcaac < 1:3472785/70‑1 (MQ=255) ttttGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTcaaca > 1:1990566/1‑69 (MQ=255) cTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACt > 1:1072313/1‑71 (MQ=255) cTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACt > 1:2105815/1‑71 (MQ=255) cTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACt > 1:3201883/1‑71 (MQ=255) cTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACt > 1:634875/1‑71 (MQ=255) cATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACttt > 1:3841725/1‑71 (MQ=255) aTAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACtt < 1:1998809/69‑1 (MQ=255) tAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTc < 1:109124/70‑1 (MQ=255) aaaTCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAg > 1:2681105/1‑70 (MQ=255) cgcgCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGtt < 1:1942634/71‑1 (MQ=255) cgcgCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGtt < 1:1017082/71‑1 (MQ=255) cgcgCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGtt < 1:1346321/71‑1 (MQ=255) cgcgCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGtt < 1:1081283/71‑1 (MQ=255) cgcgCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGtt < 1:370920/71‑1 (MQ=255) gcgcCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGtt < 1:1639701/70‑1 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatacga > 1:210179/1‑70 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatacga > 1:3267777/1‑70 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatacga > 1:974643/1‑70 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatacga > 1:2709372/1‑70 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTAAGTACCGTTAatacga > 1:3603882/1‑70 (MQ=255) caagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatac > 1:1777898/1‑67 (MQ=255) agccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGt < 1:575097/59‑1 (MQ=255) agccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGt < 1:2786556/59‑1 (MQ=255) agccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatacgatacg < 1:924086/71‑1 (MQ=255) ccaaccaaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTa > 1:2905071/1‑59 (MQ=255) ccaaccaaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTa > 1:1896771/1‑59 (MQ=255) ccaaccaaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTa > 1:2909951/1‑59 (MQ=255) aaccaaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACttt < 1:2187765/46‑1 (MQ=255) aaccaaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACttt < 1:3119412/46‑1 (MQ=255) caaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGa > 1:1436599/1‑39 (MQ=255) caaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTaa > 1:461730/1‑55 (MQ=255) caaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatacga > 1:13662/1‑60 (MQ=255) aaaaGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATc < 1:2207219/71‑1 (MQ=255) aaaaGAAGTTCGGCGTTGAAGGATCGATCAACAAAg > 1:1361521/1‑36 (MQ=255) aaaaGAAGTTCGGCGTTGAAGGATCGATCAACAAAg > 1:1152731/1‑36 (MQ=255) aaaaGAAGTTCGGCGTTGAAGGATCGATCAACAAAg > 1:1079049/1‑36 (MQ=255) aaaaGAAGTTCGGCGTTGAAGGATCGATCAACAAAg > 1:346028/1‑36 (MQ=255) aaaaGAAGTTCGGCGTTGAAGGATCGATCAACAAAg > 1:1474691/1‑36 (MQ=255) aaaGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATc < 1:2677370/70‑1 (MQ=255) agaagTTCGGCGTTGAAGGATCGGTCAACAAAGGAc > 1:3529818/1‑36 (MQ=255) agaagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCaa > 1:1500599/1‑70 (MQ=255) agaagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCaa > 1:3534681/1‑70 (MQ=255) agaagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCaa > 1:709273/1‑70 (MQ=255) aagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAg < 1:165027/71‑1 (MQ=255) aagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAg < 1:2389734/71‑1 (MQ=255) gTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGat < 1:719021/71‑1 (MQ=255) gTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGat < 1:3575040/71‑1 (MQ=255) gTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGat < 1:2035957/71‑1 (MQ=255) gTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGat < 1:369587/71‑1 (MQ=255) gTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGat < 1:1814101/71‑1 (MQ=255) gTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGa < 1:2507049/70‑1 (MQ=255) ttCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGtt < 1:3916870/44‑1 (MQ=255) cGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATAtt > 1:1285259/1‑71 (MQ=255) cGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATAtt > 1:3937505/1‑71 (MQ=255) cGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTaa > 1:2992943/1‑44 (MQ=255) cGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTaa > 1:2988222/1‑44 (MQ=255) cGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTaa > 1:3201499/1‑44 (MQ=255) cGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGatat < 1:1908570/70‑1 (MQ=255) cGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGatat > 1:2226151/1‑70 (MQ=255) cGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGatat < 1:2289292/70‑1 (MQ=255) cGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGata > 1:981391/1‑69 (MQ=255) cGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGata > 1:3629554/1‑69 (MQ=255) cGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGata > 1:3872580/1‑69 (MQ=255) cGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGata > 1:1290881/1‑69 (MQ=255) cGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGata > 1:2653938/1‑69 (MQ=255) cGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATAtt > 1:3180337/1‑71 (MQ=255) ggCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGatat < 1:44250/69‑1 (MQ=255) ggCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTg < 1:3377342/71‑1 (MQ=255) ggCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTg < 1:2164715/71‑1 (MQ=255) ggCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTg < 1:900989/71‑1 (MQ=255) gCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGc < 1:3164238/71‑1 (MQ=255) gCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGc < 1:3726898/71‑1 (MQ=255) gCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGc < 1:2218725/71‑1 (MQ=255) gTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAg < 1:1826972/71‑1 (MQ=255) ttGAAGGATCGGTCAACAAAGGACTTTCAGTACCGtt < 1:61791/37‑1 (MQ=255) ttGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACa < 1:952165/60‑1 (MQ=255) ttGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACa < 1:322440/60‑1 (MQ=255) gAAGGATCGGTCAACAAAGGACTTTCAGTACCGtt < 1:3610489/35‑1 (MQ=255) gAAGGATCGGTCAACAAAGGACTTTCAGTACCGtt < 1:2089552/35‑1 (MQ=255) gAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGat > 1:3365599/1‑61 (MQ=255) gAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGat > 1:3274154/1‑61 (MQ=255) gAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGat > 1:3038854/1‑61 (MQ=255) gAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTgg > 1:3315764/1‑71 (MQ=255) aaGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGat < 1:2494510/60‑1 (MQ=255) aaGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGat < 1:2546440/60‑1 (MQ=255) aaGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGat < 1:2641888/60‑1 (MQ=255) aaGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGat < 1:3488022/60‑1 (MQ=255) aGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGat < 1:209146/59‑1 (MQ=255) aGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGc > 1:1234220/1‑71 (MQ=255) tCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGata > 1:1355125/1‑56 (MQ=255) tCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGata > 1:94964/1‑56 (MQ=255) tCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCAtt < 1:243120/71‑1 (MQ=255) tCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCAtt < 1:162054/71‑1 (MQ=255) tCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCAtt < 1:2627583/71‑1 (MQ=255) tCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCAt > 1:116043/1‑70 (MQ=255) ggTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGata > 1:2480910/1‑54 (MQ=255) | GTATTTTTTGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCATT > minE/2321631‑2321765 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |