Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,359,867 G→A 50.5% noncoding (1228/2905 nt) rrlA ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,359,8670GA50.5% ‑3.2 / 166.6 107noncoding (1228/2905 nt)rrlA23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (33/20);  new base A (31/23);  total (64/43)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGC  >  minE/2359817‑2359935
                                                  |                                                                    
cGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTcaca                                                  <  1:1330886/71‑1 (MQ=255)
     taacGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                              >  1:887778/4‑70 (MQ=11)
     tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                              <  1:2762638/70‑1 (MQ=35)
     tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                              <  1:277593/70‑1 (MQ=35)
     tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                              >  1:2143941/1‑70 (MQ=255)
     tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                              >  1:1418373/1‑70 (MQ=255)
       tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACATCACACCTTCACAGGCTTa                                            >  1:3335971/1‑70 (MQ=25)
        cGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTa                                            >  1:323696/1‑69 (MQ=255)
         gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTa                                            >  1:2536669/1‑68 (MQ=255)
         gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTa                                            >  1:3659205/1‑68 (MQ=255)
         gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTa                                            >  1:1434570/1‑68 (MQ=255)
         gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACa                                          <  1:1123469/70‑1 (MQ=255)
         gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACa                                          <  1:1893590/70‑1 (MQ=255)
         gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAg                                         <  1:558770/71‑1 (MQ=255)
         gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAg                                         <  1:3289532/71‑1 (MQ=255)
             cTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAAc                                      <  1:2296680/70‑1 (MQ=255)
              ttATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                         <  1:2075196/66‑1 (MQ=35)
              ttATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACg                                     >  1:3772624/1‑70 (MQ=35)
              ttATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTGACAGTACACCTTCACAGGCTTACAg                                         <  1:2979961/66‑1 (MQ=11)
              ttATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAg                                         <  1:2622659/66‑1 (MQ=255)
              ttATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAg                                         <  1:1578504/66‑1 (MQ=255)
                aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGc                                    <  1:4034623/69‑1 (MQ=255)
                aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGc                                    <  1:2865072/69‑1 (MQ=255)
                aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGc                                    <  1:3394110/69‑1 (MQ=255)
                aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGc                                    <  1:1095512/69‑1 (MQ=255)
                aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGc                                    <  1:4037246/69‑1 (MQ=255)
                aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                  >  1:3059245/1‑71 (MQ=255)
                aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                  >  1:1848612/1‑71 (MQ=255)
                aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                  >  1:1928620/1‑71 (MQ=255)
                aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                  >  1:1271045/1‑71 (MQ=255)
                aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                  >  1:1041004/1‑71 (MQ=255)
                aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                  >  1:2217369/1‑71 (MQ=255)
                 tGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAg                                         <  1:3910962/63‑1 (MQ=255)
                  gTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTccc                                >  1:3994955/1‑71 (MQ=34)
                      gCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAc                            >  1:650659/1‑71 (MQ=34)
                      gCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAc                            >  1:2620020/1‑71 (MQ=34)
                       cATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAcc                           >  1:1571295/1‑71 (MQ=34)
                       cATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAcc                           >  1:1277783/1‑71 (MQ=34)
                         ttCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCca                         <  1:1029874/71‑1 (MQ=34)
                         ttCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          <  1:1502696/70‑1 (MQ=255)
                         ttCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          <  1:672014/70‑1 (MQ=255)
                         ttCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          <  1:1547257/70‑1 (MQ=255)
                         ttCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          <  1:2986783/70‑1 (MQ=255)
                         ttCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          <  1:1271121/70‑1 (MQ=255)
                          tCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACg                                     >  1:3547254/1‑58 (MQ=35)
                          tCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACg                                     >  1:2606740/1‑58 (MQ=35)
                           cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                        >  1:3918149/1‑70 (MQ=255)
                           cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                        >  1:3227307/1‑70 (MQ=255)
                           cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                        >  1:3672694/1‑70 (MQ=255)
                           cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                        >  1:376580/1‑70 (MQ=255)
                           cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                        >  1:2893520/1‑70 (MQ=255)
                           cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                        >  1:1016757/1‑70 (MQ=255)
                           cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                        >  1:2189571/1‑70 (MQ=255)
                           cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                        >  1:2448596/1‑70 (MQ=255)
                           cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                        >  1:62345/1‑70 (MQ=255)
                           cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                        >  1:2569600/1‑70 (MQ=255)
                           cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                        >  1:2011785/1‑70 (MQ=255)
                           cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                        >  1:4065186/1‑70 (MQ=255)
                            gCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac                       <  1:3052307/70‑1 (MQ=35)
                            gCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac                       <  1:538751/70‑1 (MQ=35)
                            gCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac                       <  1:1986894/70‑1 (MQ=35)
                            gCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac                       <  1:4006759/70‑1 (MQ=35)
                             cACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                     >  1:1329868/1‑71 (MQ=255)
                             cACTTCTGATACCTCCAGAATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac                       <  1:459405/69‑1 (MQ=11)
                              aCTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaac                    <  1:1486207/71‑1 (MQ=34)
                               cTTCTGATACCTCCAGCATGTCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                   <  1:3998118/71‑1 (MQ=25)
                               cTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                   <  1:3793756/71‑1 (MQ=35)
                                 tCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAc                            >  1:3125457/1‑60 (MQ=35)
                                 tCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAc                            >  1:3130093/1‑60 (MQ=35)
                                  cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                 <  1:22587/70‑1 (MQ=40)
                                  cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                 >  1:3925894/1‑70 (MQ=40)
                                  cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                 >  1:1816919/1‑70 (MQ=40)
                                  cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                 >  1:1885814/1‑70 (MQ=40)
                                       aCCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCt                                   >  1:1438513/1‑47 (MQ=38)
                                       aCCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc                                  >  1:2742273/1‑48 (MQ=38)
                                       aCCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc                                  >  1:918568/1‑48 (MQ=38)
                                       aCCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc                                  >  1:2233623/1‑48 (MQ=38)
                                       aCCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc                                  >  1:2317436/1‑48 (MQ=38)
                                       aCCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc                                  >  1:2571220/1‑48 (MQ=38)
                                       aCCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc                                  >  1:1970187/1‑48 (MQ=38)
                                       aCCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc                                  >  1:1842994/1‑48 (MQ=38)
                                       aCCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc                                  >  1:3206190/1‑48 (MQ=38)
                                        ccTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTc          <  1:1977121/71‑1 (MQ=255)
                                        ccTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTc          <  1:2711645/71‑1 (MQ=255)
                                        ccTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTc          <  1:2572152/71‑1 (MQ=255)
                                         cTCCAGCATACCTCACAGTACACCTTCACAGGCTTa                                            >  1:3811428/1‑36 (MQ=37)
                                         cTCCAGCATACCTCACAGTACACCTTCACAGGCTTa                                            >  1:1256572/1‑36 (MQ=37)
                                           ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt       >  1:3658633/1‑71 (MQ=255)
                                           ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt       >  1:1400671/1‑71 (MQ=255)
                                           ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt       >  1:1058457/1‑71 (MQ=255)
                                           ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt       >  1:2142332/1‑71 (MQ=255)
                                           ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt       >  1:1066379/1‑71 (MQ=255)
                                           ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt       >  1:3755318/1‑71 (MQ=255)
                                           ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt       >  1:4093597/1‑71 (MQ=255)
                                           ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt       >  1:1218648/1‑71 (MQ=255)
                                           ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt       >  1:2772928/1‑71 (MQ=255)
                                           ccAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                     <  1:536743/57‑1 (MQ=11)
                                                 tGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGc  <  1:4070342/70‑1 (MQ=255)
                                                 tGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGc  <  1:1201955/70‑1 (MQ=255)
                                                 tGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGc  <  1:302971/70‑1 (MQ=255)
                                                  |                                                                    
CGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGC  >  minE/2359817‑2359935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑