| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,384,489 | G→A | 13.3% | L358L (CTG→TTG) | metE ← | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,384,489 | 0 | G | A | 13.3% | 252.4 / 25.1 | 90 | L358L (CTG→TTG) | metE | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (39/39); new base A (5/7); total (44/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTC > minE/2384423‑2384558 | ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGc > 1:231762/1‑70 (MQ=255) tGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAg > 1:211605/1‑71 (MQ=255) tGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAg > 1:2679200/1‑71 (MQ=255) aaTCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGt > 1:1793144/1‑70 (MQ=255) cTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGGCAGTTCATGGCAttt > 1:3048397/1‑71 (MQ=255) cTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCAttt > 1:3963145/1‑71 (MQ=255) cTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCAttt > 1:3414020/1‑71 (MQ=255) ccACTCTGACAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCAtttt > 1:2709073/1‑70 (MQ=255) cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:3199369/71‑1 (MQ=255) cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:2357012/71‑1 (MQ=255) cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:2450233/71‑1 (MQ=255) cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg > 1:1663265/1‑71 (MQ=255) cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:2969347/71‑1 (MQ=255) cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:307096/71‑1 (MQ=255) cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg > 1:1458930/1‑71 (MQ=255) cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:3219195/71‑1 (MQ=255) cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:3406489/71‑1 (MQ=255) cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:1266250/71‑1 (MQ=255) cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:3489890/71‑1 (MQ=255) cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:1189413/71‑1 (MQ=255) cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:4081980/71‑1 (MQ=255) cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:691546/71‑1 (MQ=255) cACTCTGCCAGAGCTGCCGTGTAACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg > 1:1128089/1‑71 (MQ=255) ctGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAgg > 1:1599981/1‑71 (MQ=255) tGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCAttttt > 1:282847/1‑65 (MQ=255) tGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCAttttt > 1:549880/1‑65 (MQ=255) gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:3786761/1‑71 (MQ=255) gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:3858875/1‑71 (MQ=255) gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:2436248/1‑71 (MQ=255) gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:472278/1‑71 (MQ=255) gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:1880534/1‑71 (MQ=255) gCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg > 1:2982017/1‑59 (MQ=255) gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCAt > 1:56710/1‑46 (MQ=255) gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaaa > 1:1520747/1‑71 (MQ=255) cGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAcc < 1:291584/71‑1 (MQ=255) gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaaa > 1:3581046/1‑68 (MQ=255) gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAc > 1:2318466/1‑69 (MQ=255) gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAc > 1:3576565/1‑69 (MQ=255) gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAc > 1:259854/1‑69 (MQ=255) gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAc > 1:1334222/1‑69 (MQ=255) gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:3006751/68‑1 (MQ=255) gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:2837048/68‑1 (MQ=255) gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:2054522/68‑1 (MQ=255) gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:354384/68‑1 (MQ=255) gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:2547815/68‑1 (MQ=255) gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:1143566/68‑1 (MQ=255) gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:679723/68‑1 (MQ=255) tgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCAttt < 1:3202546/49‑1 (MQ=255) tgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCAttt < 1:3566495/49‑1 (MQ=255) tCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCt < 1:3268882/71‑1 (MQ=255) tCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCt < 1:3208637/71‑1 (MQ=255) tCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCt < 1:247978/71‑1 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:116741/1‑71 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt < 1:2972727/71‑1 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:503024/1‑71 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:1407526/1‑71 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:2531894/1‑71 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtt < 1:777133/70‑1 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtt > 1:2934076/1‑70 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTCCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt < 1:513235/71‑1 (MQ=255) ccacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt < 1:917543/71‑1 (MQ=255) ccacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt < 1:3164271/71‑1 (MQ=255) ccacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt < 1:2835817/71‑1 (MQ=255) ccacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt < 1:1608458/71‑1 (MQ=255) cacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTGATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt < 1:1661228/69‑1 (MQ=255) cTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGGaa < 1:1769867/58‑1 (MQ=255) cTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaa < 1:552048/58‑1 (MQ=255) cTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaa < 1:570389/58‑1 (MQ=255) cTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaa < 1:3650997/58‑1 (MQ=255) gTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCa > 1:3826583/1‑60 (MQ=255) gTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCAttt > 1:2579579/1‑71 (MQ=255) gTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACtt > 1:3077669/1‑71 (MQ=255) ttCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGc > 1:3507569/1‑53 (MQ=255) ttCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTc > 1:3027212/1‑71 (MQ=255) cAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg > 1:1091904/1‑71 (MQ=255) cAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg > 1:2418600/1‑71 (MQ=255) cAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg > 1:1541899/1‑71 (MQ=255) aGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg < 1:3886369/70‑1 (MQ=255) aGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg < 1:1219284/70‑1 (MQ=255) aTCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:1516029/71‑1 (MQ=255) aTCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:4128196/71‑1 (MQ=255) aTCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:2269720/71‑1 (MQ=255) tCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACt < 1:4097144/60‑1 (MQ=255) tCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACt < 1:58292/60‑1 (MQ=255) gcgcAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGa > 1:1824521/1‑70 (MQ=255) gcgcAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGAc > 1:3129345/1‑71 (MQ=255) gcgcAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGAc > 1:1871080/1‑71 (MQ=255) gcgcAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGAc > 1:2452422/1‑71 (MQ=255) cgcAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGAcg < 1:3668847/71‑1 (MQ=255) gcagcaGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGAcg < 1:1446628/70‑1 (MQ=255) agcagTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:2472017/64‑1 (MQ=255) agcagTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:595498/64‑1 (MQ=255) agcagTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:3724605/64‑1 (MQ=255) agTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTc > 1:2332054/1‑71 (MQ=255) gTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:3293700/60‑1 (MQ=255) | CCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTC > minE/2384423‑2384558 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |