| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 258,220 | C→T | 7.4% | P83S (CCG→TCG) | secF → | SecYEG protein translocase auxillary subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 258,220 | 0 | C | T | 7.4% | 212.3 / 4.0 | 67 | P83S (CCG→TCG) | secF | SecYEG protein translocase auxillary subunit |
| Reads supporting (aligned to +/- strand): ref base C (34/28); new base T (2/3); total (36/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGA > minE/258153‑258284 | taagCTCGAAAAACCGGCTGAAATTGACGTAATGTGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGa > 1:1592079/4‑71 (MQ=255)tACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGa > 1:686854/1‑71 (MQ=255) aaaaaCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCaa < 1:3595544/71‑1 (MQ=255) aaaaCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCaa < 1:1791895/70‑1 (MQ=255) aaaCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCaaaa < 1:3332424/71‑1 (MQ=255) cGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACttt < 1:2867943/71‑1 (MQ=255) cGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACttt < 1:500513/71‑1 (MQ=255) cGGCTGAAATTGACGTAATGCGGGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACttt < 1:2735763/71‑1 (MQ=255) tGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTgg > 1:2227712/1‑69 (MQ=255) gACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGTCGATGCTGCAAAACTTTGGTAGCAGCCa > 1:391447/1‑71 (MQ=255) gACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGTCGATGCTGCAAAACTTTGGTAGCAGCCa > 1:2215164/1‑71 (MQ=255) aCGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCAt < 1:1374942/71‑1 (MQ=255) aTGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTag > 1:2371277/1‑59 (MQ=255) aTGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTag > 1:1273833/1‑59 (MQ=255) aTGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTag > 1:3501673/1‑59 (MQ=255) tGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACtt < 1:1680632/52‑1 (MQ=255) tGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACtt < 1:3393813/52‑1 (MQ=255) tGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAcatc < 1:377504/71‑1 (MQ=255) tGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAcatc < 1:3237348/71‑1 (MQ=255) tGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAcatc < 1:3924109/71‑1 (MQ=255) gCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACtt < 1:932087/51‑1 (MQ=255) cGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGa > 1:3138500/1‑37 (MQ=255) aTGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTc > 1:1715939/1‑71 (MQ=255) gCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTcc > 1:1098539/1‑70 (MQ=255) gCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCg > 1:999022/1‑71 (MQ=255) cATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCg < 1:3670694/70‑1 (MQ=255) cATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCg < 1:2597386/70‑1 (MQ=255) cATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCg < 1:1908419/70‑1 (MQ=255) cATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCg < 1:2156252/70‑1 (MQ=255) cATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCAGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCg < 1:3160955/70‑1 (MQ=255) aTTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCg < 1:3900390/69‑1 (MQ=255) aTTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCg < 1:762545/69‑1 (MQ=255) ttGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTgg < 1:830682/44‑1 (MQ=255) ttGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCg < 1:2278350/68‑1 (MQ=255) ttGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTAt > 1:2219667/1‑71 (MQ=255) ttGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTAt > 1:857660/1‑71 (MQ=255) tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCaa < 1:3349313/35‑1 (MQ=255) tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCaa < 1:3468944/35‑1 (MQ=255) tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCaa < 1:3830610/35‑1 (MQ=255) gCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCaaaa > 1:1578900/1‑36 (MQ=255) gCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCaaaa > 1:380827/1‑36 (MQ=255) gCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACtt > 1:2951083/1‑39 (MQ=255) gCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTa > 1:304150/1‑44 (MQ=255) gCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGt > 1:4083332/1‑67 (MQ=255) gCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGt > 1:1123697/1‑67 (MQ=255) gCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGt > 1:1724235/1‑67 (MQ=255) gCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTa > 1:525064/1‑68 (MQ=255) gCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATgc > 1:3120641/1‑71 (MQ=255) gCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATgc > 1:748147/1‑71 (MQ=255) gCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATgc > 1:3108921/1‑71 (MQ=255) cAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTa > 1:1632913/1‑67 (MQ=255) aaaGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAcat > 1:3490105/1‑53 (MQ=255) aaaGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAcat > 1:3014672/1‑53 (MQ=255) aaaGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATgccgcc < 1:2367175/71‑1 (MQ=255) cGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAc < 1:2052457/46‑1 (MQ=255) ggTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAc < 1:568582/45‑1 (MQ=255) gTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGa > 1:3396300/1‑43 (MQ=255) ttGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCGTGGTCCGTa > 1:788201/1‑54 (MQ=255) ttGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATgg > 1:3928320/1‑48 (MQ=255) ttGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTc > 1:2065419/1‑50 (MQ=255) ttGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTa > 1:3557475/1‑54 (MQ=255) ttGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTa > 1:2667062/1‑54 (MQ=255) ttGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGc > 1:270146/1‑71 (MQ=255) ttaaGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGc > 1:843041/3‑70 (MQ=255) aaGAGTCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGa < 1:1465799/70‑1 (MQ=255) aaGAGTCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGa < 1:3479408/70‑1 (MQ=255) aaGAGTCGATGCTGCAAAACTTTGCTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGa < 1:1260753/70‑1 (MQ=255) | TACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGA > minE/258153‑258284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |