| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,384,511 | T→C | 17.6% | L350L (CTA→CTG) | metE ← | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,384,511 | 0 | T | C | 17.6% | 244.1 / 42.8 | 102 | L350L (CTA→CTG) | metE | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (40/44); new base C (5/13); total (45/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 102 total aligned reads displayed.
CTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAGGTCGA > minE/2384444‑2384572 | ctGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAgg > 1:1599981/1‑71 (MQ=255) gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:2436248/1‑71 (MQ=255) gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:1880534/1‑71 (MQ=255) gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:3786761/1‑71 (MQ=255) gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:3858875/1‑71 (MQ=255) gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:472278/1‑71 (MQ=255) gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaaa > 1:1520747/1‑71 (MQ=255) cGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAcc < 1:291584/71‑1 (MQ=255) gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaaa > 1:3581046/1‑68 (MQ=255) gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAc > 1:259854/1‑69 (MQ=255) gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAc > 1:1334222/1‑69 (MQ=255) gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAc > 1:3576565/1‑69 (MQ=255) gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAc > 1:2318466/1‑69 (MQ=255) gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:2837048/68‑1 (MQ=255) gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:354384/68‑1 (MQ=255) gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:2054522/68‑1 (MQ=255) gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:1143566/68‑1 (MQ=255) gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:3006751/68‑1 (MQ=255) gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:679723/68‑1 (MQ=255) gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:2547815/68‑1 (MQ=255) tCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCt < 1:3208637/71‑1 (MQ=255) tCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCt < 1:247978/71‑1 (MQ=255) tCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCt < 1:3268882/71‑1 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt < 1:2972727/71‑1 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:2531894/1‑71 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:1407526/1‑71 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:116741/1‑71 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:503024/1‑71 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtt < 1:777133/70‑1 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtt > 1:2934076/1‑70 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTCCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt < 1:513235/71‑1 (MQ=255) ccacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt < 1:3164271/71‑1 (MQ=255) ccacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt < 1:1608458/71‑1 (MQ=255) ccacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt < 1:2835817/71‑1 (MQ=255) ccacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt < 1:917543/71‑1 (MQ=255) cacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTGATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt < 1:1661228/69‑1 (MQ=255) cTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGGaa < 1:1769867/58‑1 (MQ=255) cTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaa < 1:3650997/58‑1 (MQ=255) cTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaa < 1:570389/58‑1 (MQ=255) cTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaa < 1:552048/58‑1 (MQ=255) gTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCa > 1:3826583/1‑60 (MQ=255) gTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCAttt > 1:2579579/1‑71 (MQ=255) ttCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGc > 1:3507569/1‑53 (MQ=255) ttCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTc > 1:3027212/1‑71 (MQ=255) cAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg > 1:1091904/1‑71 (MQ=255) cAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg > 1:1541899/1‑71 (MQ=255) cAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg > 1:2418600/1‑71 (MQ=255) aGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg < 1:1219284/70‑1 (MQ=255) aGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg < 1:3886369/70‑1 (MQ=255) aTCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:4128196/71‑1 (MQ=255) aTCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:1516029/71‑1 (MQ=255) aTCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:2269720/71‑1 (MQ=255) tCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACt < 1:4097144/60‑1 (MQ=255) tCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACt < 1:58292/60‑1 (MQ=255) gcgcAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGa > 1:1824521/1‑70 (MQ=255) gcgcAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGAc > 1:1871080/1‑71 (MQ=255) gcgcAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGAc > 1:3129345/1‑71 (MQ=255) gcgcAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGAc > 1:2452422/1‑71 (MQ=255) cgcAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGAcg < 1:3668847/71‑1 (MQ=255) gcagcaGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGAcg < 1:1446628/70‑1 (MQ=255) agcagTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:595498/64‑1 (MQ=255) agcagTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:3724605/64‑1 (MQ=255) agcagTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:2472017/64‑1 (MQ=255) agTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTc > 1:2332054/1‑71 (MQ=255) gTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:3293700/60‑1 (MQ=255) ccAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCAc > 1:3628290/1‑70 (MQ=255) gTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACt < 1:1079116/44‑1 (MQ=255) gTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATc < 1:3588507/52‑1 (MQ=255) gTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTc < 1:3546531/71‑1 (MQ=255) ttCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:2919233/53‑1 (MQ=255) ttCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:2733722/53‑1 (MQ=255) ttCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:3513786/53‑1 (MQ=255) ttCATGACATTTTTGCAGGGCGAAGGGAAACCAGCTTTTCACtt < 1:313001/44‑1 (MQ=37) ttCATGACATTTTTGCAGGGCGAAGGCAAACCAGCTTTTCACtt < 1:826648/44‑1 (MQ=38) ttCATGACATTTTTGCAGGGCGAAGGCAAACCAGCTTTTCACtt < 1:1579154/44‑1 (MQ=38) ttCATGACATTTTTGCAGGGCGAAGGCAAACCAGCTTTTCACtt < 1:3960536/44‑1 (MQ=38) ttCATGACATTTTTGCAGGGCGAAGGCAAACCAGCTTTTCACtt < 1:4089148/44‑1 (MQ=38) ttCATGACATTTTTGCAGGGCGAAGGCAAACCAGCTTTTCACtt < 1:482984/44‑1 (MQ=38) cATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACt < 1:915259/41‑1 (MQ=255) cATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAgg > 1:3328887/1‑71 (MQ=255) cATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAgg > 1:577107/1‑71 (MQ=255) cATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAgg > 1:1275993/1‑71 (MQ=255) cATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAgg > 1:3260301/1‑71 (MQ=255) cATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAgg > 1:3582965/1‑71 (MQ=255) cATGGCATTTTTGTAGGGCGAAGGCAAACAAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAgg > 1:34398/1‑71 (MQ=255) gCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAg > 1:1334390/1‑66 (MQ=255) gCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAg > 1:739878/1‑66 (MQ=255) gCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAg > 1:3576373/1‑66 (MQ=255) ttttGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTc < 1:2025098/53‑1 (MQ=255) tttGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAGGTCGa > 1:3482368/1‑66 (MQ=255) tttGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAGGTCGa > 1:2382712/1‑66 (MQ=255) tttGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAGGTCGa > 1:738791/1‑66 (MQ=255) tttGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAGGTCGa > 1:1635108/1‑66 (MQ=255) gTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:1308815/39‑1 (MQ=255) gTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:4028440/39‑1 (MQ=255) gTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:1600948/39‑1 (MQ=255) gTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:595694/39‑1 (MQ=255) gTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:1270719/39‑1 (MQ=255) gTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:3539545/39‑1 (MQ=255) gTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCaa < 1:1937842/39‑1 (MQ=255) | CTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCATCAAGACGCGTTTCCACGCTCAGGTCGA > minE/2384444‑2384572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |