| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,433,217 | G→A | 14.1% | D79N (GAT→AAT) | rhlB → | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,433,217 | 0 | G | A | 14.1% | 234.4 / 25.4 | 85 | D79N (GAT→AAT) | rhlB | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (32/41); new base A (7/5); total (39/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGTGAACTTGCCGTGCAGATCCA > minE/2433152‑2433284 | ccGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCg < 1:1983101/70‑1 (MQ=255)ccGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCg < 1:2416401/70‑1 (MQ=255)ccGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCg < 1:3332141/70‑1 (MQ=255) aaaCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTg < 1:1148967/71‑1 (MQ=255) aaCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGc < 1:1119492/64‑1 (MQ=255) aaCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGc < 1:2029330/64‑1 (MQ=255) aaCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGc < 1:1674833/64‑1 (MQ=255) aaCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGa < 1:2175360/71‑1 (MQ=255) aCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCGTGCGATTGCCg < 1:3030862/58‑1 (MQ=255) aCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCg < 1:620355/58‑1 (MQ=255) aCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCg < 1:4123974/58‑1 (MQ=255) aCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCg < 1:3323117/58‑1 (MQ=255) aCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGc < 1:3142846/63‑1 (MQ=255) aCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGc < 1:1937436/63‑1 (MQ=255) aCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGc < 1:1928972/63‑1 (MQ=255) aCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGc < 1:2237003/63‑1 (MQ=255) aCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGc < 1:2740291/63‑1 (MQ=255) aCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGc < 1:3914165/63‑1 (MQ=255) aCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGc < 1:4112268/63‑1 (MQ=255) cGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGc < 1:3432198/62‑1 (MQ=255) cGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAAt > 1:2385536/1‑71 (MQ=255) cGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAAt > 1:365919/1‑71 (MQ=255) cGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAAt > 1:3887193/1‑71 (MQ=255) cGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATCGGCAATCGCag < 1:503757/64‑2 (MQ=38) cGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATCGGCAATCGCag < 1:1618824/64‑2 (MQ=38) cGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATCGGCAATCGCag < 1:526147/64‑2 (MQ=38) cGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATCGGCAATCGCag < 1:2176003/64‑2 (MQ=38) gATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGAt > 1:2542076/1‑58 (MQ=255) gATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGAt > 1:3869404/1‑58 (MQ=255) gATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGAt > 1:4069336/1‑58 (MQ=255) gATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATCGGCAATCGCag < 1:2292525/63‑2 (MQ=38) gATGGAGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGAt > 1:1677702/1‑58 (MQ=255) aTGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATCGGCAATCGCagga > 1:1344618/1‑61 (MQ=37) aTGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATCGGCAATCGCagga > 1:51362/1‑61 (MQ=37) aTGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATCGGCAATCGCagga > 1:1326826/1‑61 (MQ=37) aTGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATCGGCAATCGCagga > 1:3372416/1‑61 (MQ=37) aTGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATCGGCAATCGCagga > 1:3425183/1‑61 (MQ=37) aTGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATCGGCAATCGCagga > 1:797047/1‑61 (MQ=37) aTGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATCGGCAATCGCagga > 1:2122581/1‑61 (MQ=37) aTGGCGTCTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGt < 1:3736961/65‑1 (MQ=255) gCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGc < 1:13277/70‑1 (MQ=255) gCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGc < 1:2662854/70‑1 (MQ=255) cGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGc < 1:3777667/69‑1 (MQ=255) tCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTg > 1:2263602/1‑71 (MQ=255) tCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTg > 1:1922770/1‑71 (MQ=255) ttACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGa < 1:4004773/57‑1 (MQ=255) ttACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGa < 1:2180067/57‑1 (MQ=255) ttACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGa < 1:156789/57‑1 (MQ=255) tcaCGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGa < 1:288735/55‑1 (MQ=255) cGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTa < 1:2023230/71‑1 (MQ=255) tCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCcgcg < 1:1051499/62‑1 (MQ=255) tCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCcgcg < 1:2135128/62‑1 (MQ=255) tCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCcgcg < 1:2248576/62‑1 (MQ=255) aaCGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTa > 1:2907261/1‑71 (MQ=255) aCGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTAt > 1:3175961/1‑71 (MQ=255) aCGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTAt < 1:1194000/71‑1 (MQ=255) cGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATg > 1:570603/1‑71 (MQ=255) gTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATgg < 1:2265768/71‑1 (MQ=255) gTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATgg < 1:709049/71‑1 (MQ=255) tttCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGc < 1:749032/39‑1 (MQ=255) tttCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATgg > 1:1655707/1‑70 (MQ=255) tttCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATgg > 1:1534492/1‑70 (MQ=255) tCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCAc > 1:3100541/1‑71 (MQ=255) tCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCAc > 1:1460130/1‑71 (MQ=255) aTTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAAt < 1:321543/61‑1 (MQ=255) tcttctCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGAcgc > 1:4125199/1‑71 (MQ=255) ttctCTCTCATCGTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGt > 1:1199112/1‑71 (MQ=255) ttctCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGt > 1:1590942/1‑71 (MQ=255) ttctCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGt > 1:184090/1‑71 (MQ=255) ttctCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGt > 1:1394298/1‑71 (MQ=255) ctctctcATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGTg < 1:2772054/70‑1 (MQ=255) tcATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCtt < 1:2875846/45‑1 (MQ=255) tcATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGTGAACTTg > 1:2113479/1‑71 (MQ=255) cATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGTGAACTTGc > 1:1241256/1‑71 (MQ=255) cTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGa > 1:976008/1‑54 (MQ=255) cTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGa > 1:3333248/1‑54 (MQ=255) cGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGTGAACTTGCCGTg > 1:1269337/1‑68 (MQ=255) cGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGTGAACTTGCCGTg > 1:1903904/1‑68 (MQ=255) cGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGTGAACTTGCCGTGc < 1:3896796/69‑1 (MQ=255) tGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGTg < 1:2389163/53‑1 (MQ=255) gCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCa > 1:4030929/1‑42 (MQ=255) gCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCa > 1:2950956/1‑42 (MQ=255) gCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCa > 1:2787724/1‑42 (MQ=255) gCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCa > 1:1797614/1‑42 (MQ=255) gCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGTGAACTTGCCGTGCAGATCCa > 1:2984549/1‑71 (MQ=255) ccGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGTGAACTTGCCTTGCAg < 1:1248963/65‑1 (MQ=255) ccGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGTGAACTTGCCGTGCAg < 1:3532566/65‑1 (MQ=255) ccGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGTGAACTTGCCGTGCAg < 1:374099/65‑1 (MQ=255) ccGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGTGAACTTGCCGTGCAg < 1:1798319/65‑1 (MQ=255) gATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATgg < 1:1839539/37‑1 (MQ=255) | CCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGACGCGTGAACTTGCCGTGCAGATCCA > minE/2433152‑2433284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |