| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,528,498 | C→T | 44.9% | D83N (GAT→AAT) | dfp ← | fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase, FMN‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,528,498 | 0 | C | T | 44.9% | 37.2 / 132.9 | 98 | D83N (GAT→AAT) | dfp | fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase, FMN‑binding |
| Reads supporting (aligned to +/- strand): ref base C (20/34); new base T (35/9); total (55/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.35e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-02 | |||||||||||
ATTCGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCGGAAAC > minE/2528433‑2528567 | aTTCGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGcc < 1:2794732/70‑1 (MQ=255) cGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt > 1:3328620/1‑71 (MQ=255) gCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt < 1:3924682/70‑1 (MQ=255) gCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt < 1:378085/70‑1 (MQ=255) gCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt < 1:2688942/70‑1 (MQ=255) gCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt < 1:3535160/70‑1 (MQ=255) ccGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAg > 1:928/1‑71 (MQ=255) ccGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAg > 1:3240737/1‑71 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCaa < 1:4067947/71‑1 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:2861028/71‑1 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:2825924/71‑1 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:380732/71‑1 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:435589/71‑1 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:1724254/71‑1 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTAGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:3797350/71‑1 (MQ=255) acacGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt < 1:2090864/55‑1 (MQ=255) cacGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:3444058/66‑1 (MQ=255) cacGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:1021432/66‑1 (MQ=255) cGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTAccc > 1:1263640/1‑57 (MQ=255) cGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAATATGGcc > 1:315530/1‑71 (MQ=255) cGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAATATGGc > 1:12469/1‑70 (MQ=255) cGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAata > 1:2852075/1‑66 (MQ=255) cGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAata > 1:2619950/1‑66 (MQ=255) cGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGcc > 1:3906751/1‑71 (MQ=255) ggCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt < 1:1299001/50‑1 (MQ=255) ggCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCa > 1:3677813/1‑71 (MQ=255) gCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:318030/61‑1 (MQ=255) aTCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGcc > 1:823897/1‑65 (MQ=255) tCAAATCTTCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt < 1:2436616/45‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCa < 1:3279501/43‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCa < 1:2990245/43‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCa < 1:1355259/43‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCa < 1:1360183/43‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCa < 1:2391534/43‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCa < 1:1569837/43‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCa < 1:3651737/38‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCa < 1:1839352/38‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCa < 1:357670/38‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCa < 1:362679/38‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCa < 1:3726472/38‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCa < 1:1372105/38‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt < 1:2117195/45‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt < 1:1820900/45‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt < 1:1841823/45‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTAcc < 1:3905357/49‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTAcc < 1:2918368/49‑1 (MQ=255) cAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAATATGGCCCAt > 1:2457009/1‑66 (MQ=255) cAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAATATGGCCCAt > 1:390955/1‑66 (MQ=255) cAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAg > 1:1335488/1‑68 (MQ=255) cAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAg > 1:2611291/1‑68 (MQ=255) cAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGCgg > 1:3685614/1‑71 (MQ=255) aaaTCTGCCGTGGCTGGGGCGAGAATCACTAAATTAGCCCAtt > 1:945618/1‑43 (MQ=38) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCAtt > 1:3883889/1‑43 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCAtt > 1:4100343/1‑43 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCAtt > 1:1024186/1‑43 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCAtt > 1:1597611/1‑43 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCAtt > 1:2193793/1‑43 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCAtt > 1:2396512/1‑43 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAata > 1:1723867/1‑57 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAata > 1:911765/1‑57 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAata > 1:2327307/1‑57 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTAACCAGCTCAATATgg > 1:440720/1‑60 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAata > 1:2371231/1‑57 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:2021260/1‑71 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:887810/1‑71 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:2466092/1‑71 (MQ=255) aTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:3037775/53‑1 (MQ=255) aTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:3535484/53‑1 (MQ=255) tCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCa > 1:1281673/1‑38 (MQ=255) tCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCa > 1:2425121/1‑38 (MQ=255) tCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCa > 1:2994805/1‑38 (MQ=255) tCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCa > 1:3527814/1‑38 (MQ=255) tCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCa > 1:916367/1‑38 (MQ=255) tCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCa > 1:3375976/1‑38 (MQ=255) tGCCGTGGCATGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:4116351/50‑1 (MQ=255) tGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt < 1:3992509/38‑1 (MQ=255) tGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCa > 1:691870/1‑44 (MQ=255) tGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCa > 1:3035124/1‑44 (MQ=255) tGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCa > 1:3862530/1‑44 (MQ=255) tGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCa > 1:888776/1‑44 (MQ=255) tGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAata > 1:393667/1‑52 (MQ=255) tGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCgg > 1:2795580/1‑71 (MQ=255) gggCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTc < 1:3264525/58‑1 (MQ=255) gagaATCACTAAATTAGCCCATTTACCCAGCTCAata > 1:1382722/1‑37 (MQ=255) gagaATCACTAAATTAGCCCATTTACCCAGCTCAata > 1:4071160/1‑37 (MQ=255) gagaATCACTAAATTAGCCCATTTACCCAGCTCAata > 1:1729216/1‑37 (MQ=255) agaATCACTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATa > 1:3303806/1‑45 (MQ=255) agaATCACTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATa > 1:697982/1‑45 (MQ=255) agaATCACTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCcag > 1:2606615/1‑68 (MQ=255) aaTCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGt > 1:2947871/1‑62 (MQ=255) aCTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGCgg > 1:1220961/1‑43 (MQ=255) aCTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGCgg > 1:3484386/1‑43 (MQ=255) aaTTAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCGGa < 1:3442868/70‑1 (MQ=255) aaTCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCGGa < 1:196401/70‑1 (MQ=255) aTCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCcagcag < 1:3081617/60‑1 (MQ=255) tCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCGGAAAc > 1:2313158/1‑71 (MQ=255) tCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCGGAAAc > 1:3302677/1‑71 (MQ=255) tCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCGGAAAc > 1:3297954/1‑71 (MQ=255) | ATTCGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCGGAAAC > minE/2528433‑2528567 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |