| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 46,543 | Δ1 bp | 100% | coding (1097/1863 nt) | kefC → | potassium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 46,543 | 0 | T | . | 100.0% | 48.7 / NA | 12 | coding (1097/1863 nt) | kefC | potassium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (12/0); total (12/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTCA > minE/46514‑46581 | ggCGAAATCGCTGACCCTGGCGGTGGCGC‑GTCGATGGCAGCTACGCCGATTCTGCTGGTGATc > 1:1662198/1‑63 (MQ=255)ggCGAAATCGCTGACCCTGGCGGTGGCGC‑GTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTc > 1:145877/1‑66 (MQ=255)ggCGAAATCGCTGACCCTGGCGGTGGCGC‑GTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTc > 1:619951/1‑66 (MQ=255)ggCGAAATCGCTGACCCTGGCGGTGGCGC‑GTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTCa > 1:1557301/1‑67 (MQ=255)ggCGAAATCGCTGACCCTGGCGGTGGCGC‑GTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTCa > 1:1638584/1‑67 (MQ=255)ggCGAAATCGCTGACCCTGGCGGTGGCGC‑GTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTCa > 1:170922/1‑67 (MQ=255)ggCGAAATCGCTGACCCTGGCGGTGGCGC‑GTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTCa > 1:1777951/1‑67 (MQ=255)ggCGAAATCGCTGACCCTGGCGGTGGCGC‑GTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTCa > 1:185355/1‑67 (MQ=255)ggCGAAATCGCTGACCCTGGCGGTGGCGC‑GTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTCa > 1:2088993/1‑67 (MQ=255)ggCGAAATCGCTGACCCTGGCGGTGGCGC‑GTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTCa > 1:411258/1‑67 (MQ=255)ggCGAAATCGCTGACCCTGGCGGTGGCGC‑GTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTCa > 1:612756/1‑67 (MQ=255)ggCGAAATCGCTGACCCTGGCGGTGGCGC‑GTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTCa > 1:863512/1‑67 (MQ=255) | GGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTCA > minE/46514‑46581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |