| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,356,182 | Δ1 bp | 100% | coding (444/1035 nt) | yehA ← | predicted fimbrial‑like adhesin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,356,182 | 0 | A | . | 89.5% | 130.9 / 11.9 | 38 | coding (444/1035 nt) | yehA | predicted fimbrial‑like adhesin protein |
| Reads supporting (aligned to +/- strand): ref base A (4/0); new base . (0/34); total (4/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.35e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCA > minE/1356139‑1356221 | aTGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAg > 1:848996/1‑66 (MQ=255)aTGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGc > 1:149631/1‑67 (MQ=255) aCTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCAc > 1:1599960/1‑66 (MQ=255) aCTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCAc > 1:484881/1‑66 (MQ=255) aaaCTCAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:168155/65‑1 (MQ=255) aaaCACAGTAGAAATTTTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1077931/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:82697/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:75417/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:751429/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:662981/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:860383/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:8965/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:432680/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1025413/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:274002/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:228573/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:973272/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1941633/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1928271/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1891591/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1874318/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1491977/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1099255/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1109684/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1121858/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1241015/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1287280/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1403830/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1444673/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1864583/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1544016/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1551045/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1611019/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1649535/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1722546/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1753272/65‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:1780047/65‑1 (MQ=255) tAGAAATTGTACTGCGGC‑CTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:792160/57‑1 (MQ=255) | ATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCA > minE/1356139‑1356221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |