Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,520,182 Δ1 bp 100% coding (707/813 nt) truA ← pseudouridylate synthase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,520,1820G.100.0% 11.7 / NA 4coding (707/813 nt)truApseudouridylate synthase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (0/4);  total (0/4)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGC  >  minE/1520157‑1520224
                         |                                          
cccTTCCGCTTTTGCCGTTGCTGCCCCAGCTTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc  <  1:1883359/67‑1 (MQ=255)
cccTTCCGCTTTTGCCGTTGCTGCCCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc  <  1:1110294/67‑1 (MQ=255)
cccTTCCGCTTTTGCCGTTGCTGCCCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc  <  1:756035/67‑1 (MQ=255)
 ccTTCCGCTTTTGCCGTTGCTGCCCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc  <  1:367982/66‑1 (MQ=255)
                         |                                          
CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGC  >  minE/1520157‑1520224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: