Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,520,182 | Δ1 bp | 100% | coding (707/813 nt) | truA ← | pseudouridylate synthase I |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,520,182 | 0 | G | . | 100.0% | 11.7 / NA | 4 | coding (707/813 nt) | truA | pseudouridylate synthase I |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (0/4); total (0/4) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGC > minE/1520157‑1520224 | cccTTCCGCTTTTGCCGTTGCTGCC‑CCAGCTTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc < 1:1883359/67‑1 (MQ=255) cccTTCCGCTTTTGCCGTTGCTGCC‑CCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc < 1:1110294/67‑1 (MQ=255) cccTTCCGCTTTTGCCGTTGCTGCC‑CCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc < 1:756035/67‑1 (MQ=255) ccTTCCGCTTTTGCCGTTGCTGCC‑CCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc < 1:367982/66‑1 (MQ=255) | CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGC > minE/1520157‑1520224 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |