| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,744,420 | Δ1 bp | 100% | coding (1315/1623 nt) | nadB → | quinolinate synthase, L‑aspartate oxidase (B protein) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,744,420 | 0 | A | . | 96.0% | 101.4 / ‑2.1 | 25 | coding (1315/1623 nt) | nadB | quinolinate synthase, L‑aspartate oxidase (B protein) subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (0/24); total (0/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAA > minE/1744374‑1744478 | tGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTg < 1:1363033/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:995892/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:1223313/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:948395/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:834417/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:767587/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:649057/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:613595/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:609250/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:585930/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:572765/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:487253/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:275356/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:2126640/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:1887239/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:1787372/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:176986/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:1738208/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:1688921/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:1679903/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:1675973/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:1400153/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATAACGTTGGCATTGTGCGCACAACGaa < 1:2068498/67‑1 (MQ=255) aTTCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATCTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:817480/67‑1 (MQ=255) ttCAGCAT‑ACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa < 1:1536268/66‑1 (MQ=255) | TGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAA > minE/1744374‑1744478 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |