| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,789,346 | Δ1 bp | 100% | coding (483/1269 nt) | ygaF → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,789,346 | 0 | A | . | 78.6% | 27.4 / 9.2 | 14 | coding (483/1269 nt) | ygaF | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base . (0/11); total (3/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.75e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATAACGC > minE/1789299‑1789369 | aGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAG‑GGCGGTGaaa < 1:1184162/57‑1 (MQ=255)aGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAG‑GGCGGTGaaa < 1:1493976/57‑1 (MQ=255)aGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAG‑GGCGGTGaaa < 1:1525162/57‑1 (MQ=255)aGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAG‑GGCGGTGaaa < 1:1588428/57‑1 (MQ=255)aGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAG‑GGCGGTGaaa < 1:1634846/57‑1 (MQ=255)aGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAG‑GGCGGTGaaa < 1:1723159/57‑1 (MQ=255)aGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAG‑GGCGGTGaaa < 1:1804112/57‑1 (MQ=255)aGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAG‑GGCGGTGaaa < 1:393106/57‑1 (MQ=255)aGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAG‑GGCGGTGaaa < 1:617591/57‑1 (MQ=255)aGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAG‑GGCGGTGaaa < 1:906164/57‑1 (MQ=255) ccGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAG‑GGCGGTGaaa < 1:249856/52‑1 (MQ=255) cgcgACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATAACGc > 1:802083/1‑65 (MQ=255) cgcgACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATAACGc > 1:857710/1‑65 (MQ=255) cgcgACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATAACGc > 1:946574/1‑65 (MQ=255) | AGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATAACGC > minE/1789299‑1789369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |