Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	410,359:1	+C	100%	coding (1997/2583 nt)	leuS ←	leucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	410,359	1	.	C	83.3%	53.1 / 5.1	18	coding (1997/2583 nt)	leuS	leucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base . (3/0);  new base C (0/15);  total (3/15)
Fisher's exact test for biased strand distribution p-value = 1.23e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

TGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAG‑CCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCA  >  minE/410317‑410419                                            |                                                             tgCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAG‑CCCCTTCCACACCGGATTCCTGcc                                      >  1:719630/1‑67 (MQ=255) tgCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAG‑CCCCTTCCACACCGGATTCCTGcc                                      >  1:690510/1‑67 (MQ=255) tgCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAG‑CCCCTTCCACACCGGATTCCTGcc                                      >  1:575405/1‑67 (MQ=255)                                      ggTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAcatca  <  1:246605/67‑1 (MQ=255)                                      ggTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAcatca  <  1:96307/67‑1 (MQ=255)                                      ggTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAcatca  <  1:88019/67‑1 (MQ=255)                                      ggTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAcatca  <  1:721193/67‑1 (MQ=255)                                      ggTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAcatca  <  1:477503/67‑1 (MQ=255)                                      ggTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAcatca  <  1:423488/67‑1 (MQ=255)                                      ggTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAcatca  <  1:1001836/67‑1 (MQ=255)                                      ggTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAcatca  <  1:1922849/67‑1 (MQ=255)                                      ggTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAcatca  <  1:1790424/67‑1 (MQ=255)                                      ggTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAcatca  <  1:1707763/67‑1 (MQ=255)                                      ggTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAcatca  <  1:1413414/67‑1 (MQ=255)                                      ggTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAcatca  <  1:134746/67‑1 (MQ=255)                                      ggTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAcatca  <  1:1191665/67‑1 (MQ=255)                                      ggTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAATCATATCAGCCGGAGAAGCAAAcatca  <  1:1875956/67‑1 (MQ=255)                                       gTTAGCCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAcatca  <  1:1396262/66‑1 (MQ=255)                                            |                                                             TGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAG‑CCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCA  >  minE/410317‑410419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑