| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 419,065:1 | +G | 100% | coding (664/1176 nt) | ubiF → | 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 419,065 | 1 | . | G | 96.1% | 189.6 / 0.2 | 51 | coding (664/1176 nt) | ubiF | 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (0/49); total (0/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
GCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACT‑GGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAAT > minE/419016‑419124 | gcaATTTACT‑CGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACT‑GGGCATCGCTGGTGTGGt < 1:1821961/67‑1 (MQ=255) caATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACT‑GGGCATCGCTGGTGTGGt < 1:442435/67‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:571033/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:2076136/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:2114142/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:2119613/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:213076/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:236609/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:327095/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:330537/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:371969/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:397015/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:54496/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:2024687/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:57191/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:680169/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:683846/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:686793/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:715727/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:729651/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:78831/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:835879/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:866201/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:900620/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:938841/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:973730/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1682199/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1038040/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1179146/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1195011/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1250447/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1275368/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1279684/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1351919/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1408099/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1530256/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1593361/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1673574/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1022551/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1753360/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1830838/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1837419/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1896339/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1919084/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1922124/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1963916/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1984607/66‑1 (MQ=255) aTAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1997662/66‑1 (MQ=255) tAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:412445/65‑1 (MQ=255) tAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:1471037/65‑1 (MQ=255) tAACTGGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaat < 1:2002336/65‑1 (MQ=255) | GCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACT‑GGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAAT > minE/419016‑419124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |