Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 570,905:1 +A 100% coding (652/900 nt) ybjE ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE570,9051.A75.0% 14.8 / 2.7 8coding (652/900 nt)ybjEpredicted transporter
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base A (6/0);  total (8/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

AATCAGTTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACC‑AAAAGATTCGGTCAATAAAATAC  >  minE/570852‑570928
                                                      |                       
aaTCAGTTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAAGATTCgg              >  1:1623768/1‑66 (MQ=255)
aaTCAGTTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAAGATTCgg              >  1:1876269/1‑66 (MQ=255)
aaTCAGTTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAAGATTCgg              >  1:2122611/1‑66 (MQ=255)
aaTCAGTTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAAGATTCgg              >  1:642653/1‑66 (MQ=255)
aaTCAGTTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAAGATTCgg              >  1:756789/1‑66 (MQ=255)
aaTCAGTTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAAGATTCg               >  1:986689/1‑65 (MQ=255)
          cgtgCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACC‑AAAAGATTCGGTCAATAAAATAc  >  1:1220813/4‑67 (MQ=255)
          cGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACC‑AAAAGATTCGGTCAATAAAATAc  >  1:1642497/1‑67 (MQ=255)
                                                      |                       
AATCAGTTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACC‑AAAAGATTCGGTCAATAAAATAC  >  minE/570852‑570928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑