Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 619,771:1 +C 100% coding (1078/1674 nt) rpsA → 30S ribosomal subunit protein S1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE619,7711.C100.0% 187.2 / NA 49T360P (ACC→CCC) rpsA30S ribosomal subunit protein S1
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (0/49);  total (0/49)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAA‑CCCACAACAAGGGCGACC  >  minE/619724‑619789
                                                |                  
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:444595/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1003173/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1994917/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:2013475/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:254646/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:261131/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:270167/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:279287/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:28773/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:363197/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:416838/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:423702/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1954214/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:524911/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:531991/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:566162/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:69204/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:702702/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:728637/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:74689/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:802702/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:81955/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:934997/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:95495/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1514688/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1062143/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1092082/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1162210/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1184989/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1258609/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:133595/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1370918/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1384716/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1386731/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1403077/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1919773/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1579235/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1625627/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1636895/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1724955/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1741487/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1754009/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1794229/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1799574/67‑1 (MQ=255)
tGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1864611/67‑1 (MQ=255)
 gggTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:327143/66‑1 (MQ=255)
 gggTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1870288/66‑1 (MQ=255)
                       aaCCCGTGGCAGCAGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:1132776/44‑1 (MQ=38)
                               gcagcaGTTCGCGGAAACCCCACAACAAGGGCGAcc  <  1:851314/36‑1 (MQ=255)
                                                |                  
TGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAA‑CCCACAACAAGGGCGACC  >  minE/619724‑619789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑