| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 91,086:1 | +C | 100% | coding (1042/1152 nt) | ftsZ → | GTP‑binding tubulin‑like cell division protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 91,086 | 1 | . | C | 91.3% | 72.9 / 0.8 | 23 | coding (1042/1152 nt) | ftsZ | GTP‑binding tubulin‑like cell division protein |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (21/0); total (21/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
CAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGA‑CCCAGGAGCAGAAGCCGGTTGCTAAAGTCG > minE/91038‑91116 | cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGcc > 1:1627622/1‑66 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:1855718/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:94131/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:888911/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:884833/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:772078/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:479647/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:47733/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:367226/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:321047/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:249803/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:1988852/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:1007299/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:1818382/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:173069/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:1729832/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:1582561/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:1321176/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:1122607/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:1077106/1‑67 (MQ=255)cagcagCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCCAGGAGCAGAAGCCg > 1:105451/1‑67 (MQ=255) cGCTACCAGCAGCATGGGATGGCTCCGCTGA‑CCCAGGAGCAGAAGCCGGTTGCTAAAGTCg < 1:1533192/61‑1 (MQ=255) cGCTACCAGCAGCATGGGATGGCTCCGCTGA‑CCCAGGAGCAGAAGCCGGTTGCTAAAGTCg < 1:816956/61‑1 (MQ=255) | CAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGA‑CCCAGGAGCAGAAGCCGGTTGCTAAAGTCG > minE/91038‑91116 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |