| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 874,234:1 | +G | 100% | pseudogene (121/129 nt) pseudogene (121/299 nt) pseudogene (11/189 nt) |
yciX → yciX → yciX → |
ECK1270:JW5198:b4522; hypothetical protein, N‑ter fragment ECK1270:JW5198+JW5199:b4574; hypothetical protein ECK1270:JW5199:b4523; hypothetical protein, C‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 874,234 | 1 | . | G | 78.1% | 222.7 / 52.2 | 73 | pseudogene (121/129 nt) pseudogene (121/299 nt) pseudogene (11/189 nt) | yciX yciX yciX | ECK1270:JW5198:b4522; hypothetical protein, N‑ter fragment ECK1270:JW5198+JW5199:b4574; hypothetical protein ECK1270:JW5199:b4523; hypothetical protein, C‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base . (2/14); new base G (0/57); total (2/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
TTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCC > minE/874179‑874284 | ttttCTTGGTTCTTCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTa < 1:1882430/67‑1 (MQ=255)ttttCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGT‑GGGGTTAACTa < 1:233280/67‑1 (MQ=255)ttttCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTa < 1:1486508/67‑1 (MQ=255)ttttCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTa < 1:432961/67‑1 (MQ=255)ttttCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTa < 1:2062942/67‑1 (MQ=255)ttttCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTa < 1:1979823/67‑1 (MQ=255)ttttCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTa < 1:1670393/67‑1 (MQ=255)ttttCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTa < 1:670815/67‑1 (MQ=255)ttttCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTa < 1:1174763/67‑1 (MQ=255) tttCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTa < 1:1842784/66‑1 (MQ=255) tttCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTATGGTCGGTCAGGAGCAAc > 1:1778799/1‑67 (MQ=255) tttCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTATGGTCGGTCAGGAGCAAc > 1:1132751/1‑67 (MQ=255) gTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCAcc < 1:1799017/67‑1 (MQ=255) gTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCAcc < 1:1957820/67‑1 (MQ=255) gTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCAcc < 1:251795/67‑1 (MQ=255) gTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCAcc < 1:344309/67‑1 (MQ=255) gATTTGGAAAGTGTGGGGGTTAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:730310/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1979214/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1928321/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1903850/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1902631/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:412620/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:435944/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:655809/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:981629/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:928881/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:927676/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:899517/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:878251/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:719730/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:664529/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:663052/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:423153/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:650032/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:605721/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:541083/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:540161/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:4676/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:456745/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1822625/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1402986/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1049140/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1067787/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1074290/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1085770/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1093872/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1152175/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:121507/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1217530/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1225367/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1330288/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:134133/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:134623/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1368781/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1373514/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1385066/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1594926/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1840773/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1022419/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1812531/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1745093/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1622777/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1605200/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1596075/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1850549/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1592478/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1491488/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1490206/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:148098/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1467279/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1419366/65‑1 (MQ=255) gATTTGGAAAGTGTGGGGGATAACTATGGTCGGGCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:832952/65‑1 (MQ=255) aTTTGGAAAGTGTGGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGcc < 1:1544519/64‑1 (MQ=255) | TTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGT‑GGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCC > minE/874179‑874284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |