Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	176,576:1	+G	100%	coding (1206/1353 nt)	yaeL →	zinc metallopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	176,576	1	.	G	100.0%	123.0 / NA	33	D402E (GAC→GAG)	yaeL	zinc metallopeptidase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (33/0);  total (33/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAACCTGTTTCCGTTGCCCGTACTTGAC‑GGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAA  >  minE/176549‑176614                             |                                       tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAGAGATCaa  >  1:303797/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1987984/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:938503/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:926423/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:926409/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:741073/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:740395/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:641987/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:595395/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:572534/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:4082/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:213641/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:2063135/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:2059326/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1998282/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1034784/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1899187/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1049631/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1092522/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:117148/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1171929/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1330342/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1572005/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1572073/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:16719/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:178277/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1838833/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1843954/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1888095/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1888205/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:1903670/1‑67 (MQ=255) tAACCTGTTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCa   >  1:25458/1‑66 (MQ=255) tAACCTATTTCCGTTGCCCGTACTTGACGGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCaa  >  1:451473/1‑67 (MQ=255)                             |                                       TAACCTGTTTCCGTTGCCCGTACTTGAC‑GGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAA  >  minE/176549‑176614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑