Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,888,707:1	+C	100%	coding (161/1341 nt)	gudD ←	(D)‑glucarate dehydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,888,707	1	.	C	100.0%	20.6 / NA	7	E54G (GAA→GGA)	gudD	(D)‑glucarate dehydratase 1
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (7/0);  total (7/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTCGCCGCCGGGAATTT‑CCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATCACAAT  >  minE/1888691‑1888750                  |                                            ctcGCCGCCGGGAATTTCCCCCTACGCCAGTGTGAc                           >  1:287862/1‑36 (MQ=37) ctcGCCGCCGGGAATTTCCCCCTACGCCAGTGTGACCAGAATTATCTTTGAt           >  1:1198330/1‑52 (MQ=255) ctcGCCGCCGGGAATTTCCCCCTACGCCAGTGTGACCAGAATTATCTTTGAt           >  1:8786/1‑52 (MQ=255) ctcGCCGCCGGGAATTTCCCCCTACGCCAGTGTGACCAGAATTATCTTTGAt           >  1:691943/1‑52 (MQ=255) ctcGCCGCCGGGAATTTCCCCCTACGCCAGTGTGACCAGAATTATCTTTGAt           >  1:1804023/1‑52 (MQ=255) ctcGCCGCCGGGAATTTCCCCCTACGCCAGTGTGACCAGAATTATCTTTGAt           >  1:1211556/1‑52 (MQ=255) ctcGCCGCCGGGAATTTCCCCCTACGCCAGTGTGACCAGAATTATCTTTGAt           >  1:1030285/1‑52 (MQ=255)                 t‑CCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATcacatt  <  1:446484/44‑3 (MQ=255)                 t‑CCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATCACAat  <  1:1601636/44‑1 (MQ=255)                 t‑CCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATCACAat  <  1:1966041/44‑1 (MQ=255)                 t‑CCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATCACAat  <  1:2099922/44‑1 (MQ=255)                 t‑CCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATCACAat  <  1:450327/44‑1 (MQ=255)                 t‑CCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATCACAat  <  1:513813/44‑1 (MQ=255)                 t‑CCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATCACAat  <  1:1205478/44‑1 (MQ=255)                 t‑CCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATCACAat  <  1:718646/44‑1 (MQ=255)                 t‑CCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATCACAat  <  1:72690/44‑1 (MQ=255)                 t‑CCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATCACAat  <  1:800970/44‑1 (MQ=255)                 t‑CCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATCACAat  <  1:812163/44‑1 (MQ=255)                  |                                            CTCGCCGCCGGGAATTT‑CCCCTACGCCAGTGTGACCAGAATTATCTTTGATAATCACAAT  >  minE/1888691‑1888750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑