Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,892,019:1 +GC 100% intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,892,0191.G100.0% 75.5 / NA 21intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (21/0);  total (21/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*minE1,892,0192.C100.0% 76.4 / NA 21intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (21/0);  total (21/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCT  >  minE/1892002‑1892066
                  ||                                               
cgctcgctGAACATATCAGCTAAGCACAGCACCGGGTCGGGTTAGACGttag                 >  1:897530/1‑50 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGCACCGGGTCGGATAAGACGTTc                  >  1:18747/1‑51 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGt                                >  1:1516974/1‑37 (MQ=25)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCg                              >  1:1816422/1‑39 (MQ=37)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGa                            >  1:1641138/1‑41 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGAt                           >  1:81418/1‑42 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa                         >  1:1896409/1‑44 (MQ=37)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                        >  1:1361661/1‑45 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                        >  1:1732334/1‑45 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc                >  1:991440/1‑53 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc                >  1:559734/1‑53 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct            >  1:154712/1‑57 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc             >  1:644141/1‑56 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt              >  1:1660138/1‑55 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt  >  1:373418/1‑67 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt  >  1:1512970/1‑67 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt  >  1:1213466/1‑67 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt  >  1:120804/1‑67 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt  >  1:1033097/1‑67 (MQ=255)
cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCATCTCATCCGACCt  >  1:1691071/1‑67 (MQ=255)
cgctcgctGAACATATCAGCTAAGCAAAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt  >  1:2147224/1‑67 (MQ=255)
                  ||                                               
CGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCT  >  minE/1892002‑1892066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑