| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,145,674:1 | +CAA | 100% | coding (1033/1626 nt) | yhbX ← | predicted hydrolase, inner membrane |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,145,674 | 1 | . | C | 77.6% | 144.8 / 35.8 | 49 | coding (1033/1626 nt) | yhbX | predicted hydrolase, inner membrane |
| Reads supporting (aligned to +/- strand): ref base . (1/10); new base C (38/0); total (39/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | minE | 2,145,674 | 2 | . | A | 77.6% | 128.8 / 35.9 | 49 | coding (1033/1626 nt) | yhbX | predicted hydrolase, inner membrane |
| Reads supporting (aligned to +/- strand): ref base . (1/10); new base A (38/0); total (39/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | minE | 2,145,674 | 3 | . | A | 77.6% | 132.3 / 35.9 | 49 | coding (1033/1626 nt) | yhbX | predicted hydrolase, inner membrane |
| Reads supporting (aligned to +/- strand): ref base . (1/10); new base A (38/0); total (39/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGTTTCTTTTGCTGCGTATTTTGCTGTAATGCTTGCGATAAATG‑CGG‑‑‑CAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGGCGCGC > minE/2145628‑2145723 ||| aGTTTCTTTTGCTGCGTATTTTGCTGTAATGCTTGCGATAAATG‑CGG‑‑‑CAACAACAATtcttca < 1:398163/63‑1 (MQ=255)aGTTTCTTTTGCTGCGTATTTTGCTGTAATGCTTGCGATAAATG‑CGG‑‑‑CAACAACAATtcatca < 1:1181830/63‑1 (MQ=255)aGTTTCTTTTGCTGCGTATTTTGCTGTAATGCTTGCGATAAATG‑CGG‑‑‑CAACAACAATtcatca < 1:886882/63‑1 (MQ=255)aGTTTCTTTTGCTGCGTATTTTGCTGTAATGCTTGCGATAAATG‑CGG‑‑‑CAACAACAATtcatca < 1:7258/63‑1 (MQ=255)aGTTTCTTTTGCTGCGTATTTTGCTGTAATGCTTGCGATAAATG‑CGG‑‑‑CAACAACAATtcatca < 1:270016/63‑1 (MQ=255)aGTTTCTTTTGCTGCGTATTTTGCTGTAATGCTTGCGATAAATG‑CGG‑‑‑CAACAACAATtcatca < 1:2096878/63‑1 (MQ=255)aGTTTCTTTTGCTGCGTATTTTGCTGTAATGCTTGCGATAAATG‑CGG‑‑‑CAACAACAATtcatca < 1:1871657/63‑1 (MQ=255)aGTTTCTTTTGCTGCGTATTTTGCTGTAATGCTTGCGATAAATG‑CGG‑‑‑CAACAACAATtcatca < 1:1843251/63‑1 (MQ=255)aGTTTCTTTTGCTGCGTATTTTGCTGTAATGCTTGCGATAAATG‑CGG‑‑‑CAACAACAATtcatca < 1:1429108/63‑1 (MQ=255)aGTTTCTTTTGCTGCGTATTTTGCTGTAATGCTTGCGATAAATG‑CGG‑‑‑CAACAACAATtcatca < 1:1228608/63‑1 (MQ=255) gCTTGCGATAAATGCCAA‑‑‑CAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcgcgc > 1:1008367/1‑67 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCct > 1:75470/1‑42 (MQ=25) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:732187/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:428505/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:432342/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:439641/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:451861/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:512699/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:66274/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:682252/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:695594/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:381624/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:815887/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:828009/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:832759/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:862001/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:891768/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:942999/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:965798/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:1354510/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:142976/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:1494807/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:1513896/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:1519980/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:163680/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:1644975/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:1671734/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:176824/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:1795343/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:1251529/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:1275878/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:2031625/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:2032806/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:2068783/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:208732/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:1268628/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:2123944/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:1265500/1‑66 (MQ=255) gCTTGCGATAAATG‑CGGCAACAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGgcg > 1:381370/1‑66 (MQ=255) ||| AGTTTCTTTTGCTGCGTATTTTGCTGTAATGCTTGCGATAAATG‑CGG‑‑‑CAACAACAATTCATCAAATCCTCTGACATAAACTGTTTCCATGGCGCGC > minE/2145628‑2145723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |