| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,743,517:1 | +TTG | 100% | coding (61/255 nt) | rpsQ → | 30S ribosomal subunit protein S17 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,743,517 | 1 | . | T | 81.3% | 226.2 / 48.9 | 75 | coding (61/255 nt) | rpsQ | 30S ribosomal subunit protein S17 |
| Reads supporting (aligned to +/- strand): ref base . (0/14); new base T (61/0); total (61/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.78e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | minE | 2,743,517 | 2 | . | T | 81.3% | 221.8 / 48.9 | 75 | coding (61/255 nt) | rpsQ | 30S ribosomal subunit protein S17 |
| Reads supporting (aligned to +/- strand): ref base . (0/14); new base T (61/0); total (61/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.78e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | minE | 2,743,517 | 3 | . | G | 81.3% | 234.3 / 43.9 | 75 | coding (61/255 nt) | rpsQ | 30S ribosomal subunit protein S17 |
| Reads supporting (aligned to +/- strand): ref base . (0/14); new base G (61/0); total (61/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.78e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAC > minE/2743464‑2743581 ||| aTAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTAt < 1:1041644/67‑1 (MQ=255)aTAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTAt < 1:1732514/67‑1 (MQ=255)aTAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTAt < 1:900719/67‑1 (MQ=255)aTAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTAt < 1:1370233/67‑1 (MQ=255)aTAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTAt < 1:2040781/67‑1 (MQ=255)aTAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTAt < 1:2123027/67‑1 (MQ=255) aagTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:2100938/3‑67 (MQ=255) aGGTCGCGTTGTTAGCGACGAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:608992/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATTGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGcaa > 1:1137160/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGATCGTTTTGTGaaa > 1:216235/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1936198/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:23914/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:220132/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:2151458/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:2105167/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:518008/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1928881/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:191553/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1911306/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1881638/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1852108/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:492584/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:503390/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:185829/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:546934/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:653919/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:662641/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:796673/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:809940/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:831957/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:851946/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:96981/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:970569/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:971147/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:972126/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:975007/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1502058/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1120339/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1130198/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1182326/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1201528/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1277199/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:10973/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1312412/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1337145/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1350593/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1374279/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:109045/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1384908/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1395111/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1481199/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1059672/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:180812/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1730545/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1707221/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1706683/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1647995/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1639830/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1560526/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1608113/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1603455/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1602085/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1594214/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1585350/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaaa > 1:1569830/1‑67 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaa > 1:732458/1‑66 (MQ=255) aGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCATTGTTGTTGTTGCTATCGAACGTTTTGTGaa > 1:828545/1‑66 (MQ=255) ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:1082645/67‑1 (MQ=255) ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:101772/67‑1 (MQ=255) ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:92148/67‑1 (MQ=255) ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:746645/67‑1 (MQ=255) ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:543138/67‑1 (MQ=255) ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:2039938/67‑1 (MQ=255) ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:2014831/67‑1 (MQ=255) ccA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAc < 1:1001083/67‑1 (MQ=255) ||| ATAAAATCCGTACTCTGCAAGGTCGCGTTGTTAGCGACAAAATGGAGAAATCCA‑‑‑TTGTTGTTGCTATCGAACGTTTTGTGAAACACCCGATCTACGGTAAATTCATCAAGCGTACGAC > minE/2743464‑2743581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |