| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,847,650:1 | +T | 100% | coding (285/315 nt) | yjeO → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,847,650 | 1 | . | T | 97.7% | 159.0 / ‑3.2 | 44 | coding (285/315 nt) | yjeO | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (43/0); total (44/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
CGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAG‑T‑TTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAAC > minE/2847611‑2847716 | cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCTGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1529213/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATc > 1:401352/1‑57 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATc > 1:1389685/1‑57 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCTGTtttgtt > 1:1887106/1‑66 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAg > 1:1144137/1‑59 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGttt > 1:2022352/1‑62 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:311169/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1088432/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1811512/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:189168/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1963359/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:2094441/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:243251/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:260593/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1786654/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:378262/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:473128/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:503964/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:761697/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:784409/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:928490/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:949548/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1440997/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1099296/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:110555/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1158921/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1198085/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1237915/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1386497/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1404772/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1456645/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1530720/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1630764/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1691948/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:1696562/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgttt > 1:173791/1‑67 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgtt > 1:2054802/1‑66 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgtt > 1:178513/1‑66 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgtt > 1:1565872/1‑66 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgtt > 1:1976028/1‑66 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgtt > 1:1384032/1‑66 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgtt > 1:1299453/1‑66 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTT‑TTTTTCTGCGCTATCAGTtttgt > 1:282040/1‑65 (MQ=255)cGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAG‑TCTTTTTCTGCGCTATCAGTtttgttt > 1:817001/1‑67 (MQ=255) g‑T‑TTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATCGGTTCATCCGGCACacaaacttacaaac < 1:1968836/67‑1 (MQ=255) | CGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAG‑T‑TTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAAC > minE/2847611‑2847716 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |