| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,866,115:1 | +C | 100% | coding (68/198 nt) | yjeT → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,866,115 | 1 | . | C | 100.0% | 24.2 / NA | 8 | Y23S (TAC→TCC) | yjeT | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (8/0); total (8/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AATTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑CCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGC > minE/2866051‑2866159 | aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1942205/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:938276/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:733538/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:502887/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:449694/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:424427/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:381458/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:2024326/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1968055/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1945870/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1720929/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:16668/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1563699/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:150795/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1500425/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:142580/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1165511/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1403711/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:140152/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1365934/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:123350/67‑1 (MQ=255)aatTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1275662/67‑1 (MQ=255)aatTCGACAATCTCGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1224322/67‑1 (MQ=255) atTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1999861/66‑1 (MQ=255) atTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:2008213/66‑1 (MQ=255) tggcGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:2037251/51‑1 (MQ=255) ccTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1644756/41‑1 (MQ=255) ttttGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑cc < 1:1576152/36‑1 (MQ=255) aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATTATCTCTGCGATGACCAATTTGc > 1:1054104/1‑66 (MQ=255) aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATGATCTCTGCg > 1:218556/1‑53 (MQ=39) aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATGATCTCTGCGATTACCAATTTg > 1:1159331/1‑65 (MQ=38) aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGc > 1:395074/1‑66 (MQ=255) aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGc > 1:1299058/1‑66 (MQ=255) aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGc > 1:330627/1‑66 (MQ=255) aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGc > 1:651068/1‑66 (MQ=255) aGGTTTAGGGCCGATGCTTTACCCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGc > 1:675354/1‑66 (MQ=255) | AATTCGACAATCTGGCTGGCGCTTGCCCTGGTTTTGGTACTGGAAGGTTTAGGGCCGATGCTTTA‑CCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGC > minE/2866051‑2866159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |