| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 300,220 | G→A | 100% | L372L (CTG→CTA) | mdlB → | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 300,220 | 0 | G | A | 90.4% | 158.2 / 6.6 | 52 | L372L (CTG→CTA) | mdlB | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (0/5); new base A (3/44); total (3/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGG > minE/300164‑300270 | aaTCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCAt > 1:2139452/1‑66 (MQ=255)aaTCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCAt > 1:434404/1‑66 (MQ=255)aaTCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATa > 1:1308628/1‑67 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGTCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1684018/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:68637/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:2098875/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:2142873/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:277342/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:370576/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:397087/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:477579/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:526799/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:572088/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1042930/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:687395/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:702602/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:705182/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:751769/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:764243/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:788584/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:793246/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:833328/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:897446/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:992001/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1408668/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1001810/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1066149/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1163889/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1208476/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1244466/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1261087/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1262859/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1317373/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1350313/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1401389/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:2093737/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1469723/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1517227/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1524821/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1529337/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1585528/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1590128/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1849661/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1900365/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1911468/66‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:2008824/66‑1 (MQ=255) tctcgcAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:343729/60‑1 (MQ=255) gcAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATggg < 1:621648/67‑1 (MQ=255) gcAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATggg < 1:699238/67‑1 (MQ=255) gcAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATggg < 1:2116128/67‑1 (MQ=255) gcAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATggg < 1:945356/67‑1 (MQ=255) cAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATggg < 1:976551/66‑1 (MQ=255) | AATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGG > minE/300164‑300270 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |