| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 335,162 | G→A | 100% | G130G (GGC→GGT) | ybaK ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 335,162 | 0 | G | A | 88.1% | 169.5 / 13.2 | 59 | G130G (GGC→GGT) | ybaK | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (4/3); new base A (0/52); total (4/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTT > minE/335107‑335223 | cGAGGATCTTTGCCAGATCTCCTGCCGCCAGTTCGATATCCACTCCGCGCTTGCCACCGGAAACATa < 1:2043837/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATa < 1:109756/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATa < 1:1114685/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:471361/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1721102/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1800920/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1983680/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:2038975/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:205954/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:2127830/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:24041/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:266630/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:299917/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:332822/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:381897/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1746911/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:477457/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:504731/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:526472/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:615248/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:666245/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:713187/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:770465/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:790454/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:929622/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:955939/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1668798/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1443639/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1495463/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1515044/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1429269/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1172378/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1171536/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1654731/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1411627/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1349372/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1285526/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1680279/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1300995/67‑1 (MQ=255)cGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1687978/67‑1 (MQ=255) gatgATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCACCGGAAACATa < 1:1049176/63‑1 (MQ=255) gAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATa < 1:1179981/66‑1 (MQ=255) atatCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATaa > 1:603705/1‑67 (MQ=255) atatCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATaa > 1:159653/1‑67 (MQ=255) atatCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATaa > 1:195500/1‑67 (MQ=255) atatCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATaa > 1:18781/1‑67 (MQ=255) ttGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGtt < 1:87998/67‑1 (MQ=255) ttGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGtt < 1:619580/67‑1 (MQ=255) ttGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGtt < 1:1134917/67‑1 (MQ=255) ttGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGtt < 1:1096640/67‑1 (MQ=255) ttGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGtt < 1:1345813/67‑1 (MQ=255) ttGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGtt < 1:1393998/67‑1 (MQ=255) ttGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGtt < 1:231950/67‑1 (MQ=255) ttGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGtt < 1:1606575/67‑1 (MQ=255) ttGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGtt < 1:1653326/67‑1 (MQ=255) ttGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGtt < 1:1976118/67‑1 (MQ=255) ttGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGtt < 1:1738745/67‑1 (MQ=255) ttGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGtt < 1:1721745/67‑1 (MQ=255) tGCCACCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGtt < 1:1198107/66‑1 (MQ=255) | CGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTT > minE/335107‑335223 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |